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Basic Study | Publishing Language: Chinese | Open Access

Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells

Jun WEN, Shuai-mei XU, Ying LIU( )
Department of Conservative Dentistry & Endodontics, Guangdong Provincial Stomatological & the Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510280, China
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Abstract

Objective

To evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).

Methods

The effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.

Results

After 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.

Conclusion

XAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.

Keywords

Dental pulp stem cellsAdipogenic differentiationWnt passwayXAV-939Cell proliferation
CLC number: R781.3 Document code: A Article ID: 2096-1456(2016)08-459-05

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 24 Issue 8,
August 2016
Pages 459-463
DOI: 10.12016/j.issn.2096-1456.2016.08.005

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Cite this article:
WEN J, XU S-m, LIU Y. Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(8): 459-463. https://doi.org/10.12016/j.issn.2096-1456.2016.08.005

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Received: 15 February 2016
Revised: 24 March 2016
Published: 20 August 2016
© 2016 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.