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Avian Research 2020, 11(1): 13 https://doi.org/10.1186/s40657-020-00195-9
Research | Open Access | Issue | Published: 30 April 2020

Comparing the intestinal bacterial communies of sympatric wintering Hooded Crane (Grus monacha) and Domestic Goose (Anser anser domesticus)

Show Author's Information Rong Fu1,2,  Xingjia Xiang1,2,  Yuanqiu Dong1,2,  Lei Cheng1,2,  Lizhi Zhou1,2( )
School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China
Anhui Province Key Laboratory of Wetland Ecological Protection and Restoration (Anhui University), Hefei 230601, China
Keywords:
High-throughput sequencing, Hooded Crane, Pathogen, Poultry, Intestinal microbiota
Cite this article:
Fu R, Xiang X, Dong Y, et al. Comparing the intestinal bacterial communies of sympatric wintering Hooded Crane (Grus monacha) and Domestic Goose (Anser anser domesticus). Avian Research, 2020, 11(1): 13. https://doi.org/10.1186/s40657-020-00195-9
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Abstract Full text About this article
Background

Gut microbiota play crucial roles in host health. Wild birds and domestic poultry often occupy sympatric habitats, which facilitate the mutual transmission of intestinal microbes. However, the distinct intestinal microbial communities between sympatric wild birds and poultry remain unknown. At present, the risk of interspecies transmission of pathogenic bacteria between wild and domestic host birds is also a research hotspot.

Methods

This study compared the intestinal bacterial communities of the overwintering Hooded Crane (Grus monacha) and the Domestic Goose (Anser anser domesticus) at Shengjin Lake, China, using Illumina high-throughput sequencing technology (Mi-Seq platform).

Results

Our results revealed that Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes and Chloroflexi were the dominant bacterial phyla in both hosts. The gut bacterial community composition differed significantly between sympatric Hooded Cranes and Domestic Geese. However, the hosts exhibited little variation in gut bacterial alpha-diversity. The relative abundance of Firmicutes was significantly higher in the guts of the Hooded Cranes, while the relative abundances of Actinobacteria, Proteobacteria, Bacteroidete and Chloroflexi were significantly higher in guts of Domestic Geese. Moreover, a total of 132 potential pathogenic operational taxonomic units (OTUs) were detected in guts of Hooded Cranes and Domestic Geese, and 13 pathogenic OTUs (9.8%) were found in both host guts. Pathogenic bacterial community composition and diversity differed significantly between hosts.

Conclusions

The results showed that the gut bacterial community composition differs significantly between sympatric Hooded Cranes and Domestic Geese. In addition, potential pathogens were detected in the guts of both Hooded Cranes and Domestic Geese, with 13 pathogenic OTUs overlapping between the two hosts, suggesting that more attention should be paid to wild birds and poultry that might increase the risk of disease transmission in conspecifics and other mixed species.


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About this article

Comparing the intestinal bacterial communies of sympatric wintering Hooded Crane (Grus monacha) and Domestic Goose (Anser anser domesticus)

Show Author's information Rong Fu1,2, Xingjia Xiang1,2, Yuanqiu Dong1,2, Lei Cheng1,2, Lizhi Zhou1,2( )
School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China
Anhui Province Key Laboratory of Wetland Ecological Protection and Restoration (Anhui University), Hefei 230601, China

Abstract

Background

Gut microbiota play crucial roles in host health. Wild birds and domestic poultry often occupy sympatric habitats, which facilitate the mutual transmission of intestinal microbes. However, the distinct intestinal microbial communities between sympatric wild birds and poultry remain unknown. At present, the risk of interspecies transmission of pathogenic bacteria between wild and domestic host birds is also a research hotspot.

Methods

This study compared the intestinal bacterial communities of the overwintering Hooded Crane (Grus monacha) and the Domestic Goose (Anser anser domesticus) at Shengjin Lake, China, using Illumina high-throughput sequencing technology (Mi-Seq platform).

Results

Our results revealed that Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes and Chloroflexi were the dominant bacterial phyla in both hosts. The gut bacterial community composition differed significantly between sympatric Hooded Cranes and Domestic Geese. However, the hosts exhibited little variation in gut bacterial alpha-diversity. The relative abundance of Firmicutes was significantly higher in the guts of the Hooded Cranes, while the relative abundances of Actinobacteria, Proteobacteria, Bacteroidete and Chloroflexi were significantly higher in guts of Domestic Geese. Moreover, a total of 132 potential pathogenic operational taxonomic units (OTUs) were detected in guts of Hooded Cranes and Domestic Geese, and 13 pathogenic OTUs (9.8%) were found in both host guts. Pathogenic bacterial community composition and diversity differed significantly between hosts.

Conclusions

The results showed that the gut bacterial community composition differs significantly between sympatric Hooded Cranes and Domestic Geese. In addition, potential pathogens were detected in the guts of both Hooded Cranes and Domestic Geese, with 13 pathogenic OTUs overlapping between the two hosts, suggesting that more attention should be paid to wild birds and poultry that might increase the risk of disease transmission in conspecifics and other mixed species.

Keywords: High-throughput sequencing, Hooded Crane, Pathogen, Poultry, Intestinal microbiota

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Publication history

Received: 21 December 2019
Accepted: 06 April 2020
Published: 30 April 2020
Issue date: January 2020

Copyright

© The Author(s) 2020.

Acknowledgements

Acknowledgements

We thank staff of Shenjin Lake National Nature Reserve for providing sample assistance in this study. We also thank Wei Chen, Weiqing Wang, Bingguo Dai, Jian Zhou, Xingran Wang, Zhengrong Zhu for helping us to collect samples.

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