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Development and application of a double-antigen Sandwich ELISA using capsid protein to detect multispecies antibodies against porcine circovirus type 3

Dexin Li1, Junhua Deng2, Yufang Li2, Liying Hao2, Wenqiang Pang3, Zhendong Zhang1, Yi Yang1, Xiangdong Li1,2( ), Kegong Tian2,3( )
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Luoyang Putai Biotechnology Co., Ltd., Luoyang 471003, China
National Research Center for Veterinary Medicine, Luoyang 471003, China
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Highlights

  • A novel double antigen sandwich ELISA (DAgS-ELISA) using baculovirus-expressed PCV3 Cap protein was established, enabling sensitive detection of PCV3 antibodies across diverse animal species.

  • A serosurvey in Chinese pigs (2022–2024) revealed a 55.7% overall PCV3 seropositivity rate, significantly higher in sows (86.3%).

  • PCV3 antibodies were detected in cattle (7.7%) and dogs (4.4%), and reported for the first time in horses, thereby significantly expanding the known host range of PCV3.

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Journal of Integrative Agriculture (JIA)
Volume 24 Issue 10,
October 2025
Pages 4105-4109
DOI: 10.1016/j.jia.2025.03.016

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Li D, Deng J, Li Y, et al. Development and application of a double-antigen Sandwich ELISA using capsid protein to detect multispecies antibodies against porcine circovirus type 3. Journal of Integrative Agriculture (JIA), 2025, 24(10): 4105-4109. https://doi.org/10.1016/j.jia.2025.03.016

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Received: 30 September 2024
Revised: 20 January 2025
Accepted: 26 February 2025
Published: 22 March 2025
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