Ascorbic acid ameliorates isoniazid-rifampicin-induced hepatocellular damage in rats

Shubham Patel; Aman Chaturvedi; Nazneen Dubey; Abhishek Shrivastava; Aditya Ganeshpurkar

doi:10.1016/j.iliver.2022.03.001

iLIVER 2022, 1(1): 72-77 https://doi.org/10.1016/j.iliver.2022.03.001

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Open Access | Issue | Published: 10 March 2022

Ascorbic acid ameliorates isoniazid-rifampicin-induced hepatocellular damage in rats

Show Author's Information Hide Author's Information Shubham Patel, Aman Chaturvedi, Nazneen Dubey, Abhishek Shrivastava, Aditya Ganeshpurkar(

)

Shri Ram Institute of Technology-Pharmacy, Jabalpur, India

Keywords:

Cytokines, Toxicity, Liver, Antitubercular, Peroxidation, Ascorbic acid

Cite this article:

Patel S, Chaturvedi A, Dubey N, et al. Ascorbic acid ameliorates isoniazid-rifampicin-induced hepatocellular damage in rats. iLIVER, 2022, 1(1): 72-77. https://doi.org/10.1016/j.iliver.2022.03.001

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Abstract Full text About this article

Abstract

Background

Phyto-constituents are widely recognized for hepatoprotective effects. Ascorbic acid is extensively studied for the radical scavenging effect. The objective of the present study was to evaluate the hepatoprotective effect of ascorbic acid against isoniazid and rifampicin-induced liver damage in rats.

Methods

Wistar rats were used in the present study. Hepato-cellular damage was produced by the administration of isoniazid (100 mg/kg) and rifampicin (100 mg/kg) for 21 days. Ascorbic acid was administered in the dose of 50, 100, and 200 mg/kg body weight. At the end of the study, blood was collected and biochemical studies were performed to determine antioxidant status.

Results

Ascorbic acid administration (50, 100, and 200 mg/kg body weight) caused restoration of serum glutamic oxaloacetic transaminase, serum glutamic-pyruvic transaminase, and serum alkaline phosphatase. The level of superoxide dismutase and catalase were also restored due to the administration of ascorbic acid. There was a decrement in the expression of tumor necrosis factor-α, interleukin-1β, interleukin-6, malondialdehyde and nitric oxide.

Conclusion

The outcome of the study established ascorbic acid as a notable hepatoprotective effect. The radical scavenging and cytokine suppression effect is the possible mechanism associated with the hepatoprotective effect of ascorbic acid.

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About this article

Ascorbic acid ameliorates isoniazid-rifampicin-induced hepatocellular damage in rats

Show Author's information Hide Author's Information Shubham Patel, Aman Chaturvedi, Nazneen Dubey, Abhishek Shrivastava, Aditya Ganeshpurkar(

)

Shri Ram Institute of Technology-Pharmacy, Jabalpur, India

Abstract

Background

Methods

Results

Conclusion

Keywords: Cytokines, Toxicity, Liver, Antitubercular, Peroxidation, Ascorbic acid

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Publication history

Received: 12 January 2022

Revised: 18 February 2022

Accepted: 02 March 2022

Published: 10 March 2022

Issue date: March 2022

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)