Simultaneous determination of 74 pesticide residues in <i>Panax notoginseng</i> by QuEChERS coupled with gas chromatography tandem mass spectrometry

Huijun Li; Jieshan Wu; Chao Chen; Wenfeng Xin; Wensheng Zhang

doi:10.1016/j.fshw.2021.02.014

Food Science and Human Wellness 2021, 10(2): 241-250 https://doi.org/10.1016/j.fshw.2021.02.014

Research Article |

Open Access | Issue | Published: 22 March 2021

Simultaneous determination of 74 pesticide residues in Panax notoginseng by QuEChERS coupled with gas chromatography tandem mass spectrometry

Show Author's Information Hide Author's Information Huijun Li^{^a^,^b^,^d}, Jieshan Wu^{^c}, Chao Chen^{^a^,^b^,^d}, Wenfeng Xin^{^e}, Wensheng Zhang^{^a^,^b^,^d}(

)

Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University at Zhuhai 519087, China

Zhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University at Zhuhai 519087, China

Gongbei customs of the People's Republic of China, Zhuhai 519000, China

Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China

National and Local United Engineering Research Center for Panax Notoginseng Resources Protection and Utilization Technology, Kunming 650000, China

Keywords:

Panax notoginseng, QuEChERS, GC-MS/MS, Pesticide residues

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Li H, Wu J, Chen C, et al. Simultaneous determination of 74 pesticide residues in Panax notoginseng by QuEChERS coupled with gas chromatography tandem mass spectrometry. Food Science and Human Wellness, 2021, 10(2): 241-250. https://doi.org/10.1016/j.fshw.2021.02.014

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Abstract

This study established a method for the simultaneous determination of 74 pesticide residues in Panax notoginseng by QuEChERS pretreatment method coupled with GC-MS/MS, and carried out pesticide residue analysis on 20 batches of market samples in China. The samples were extracted with acetonitrile, cleaned up with primary secondary amine (PSA) and octadecylsilane (C₁₈) and determined by GC-MS/MS in multiple reaction monitoring (MRM) mode. Matrix-matched calibration was recommended to combat the matrix effect. A good linearity was observed in the range of 10−500 ng/mL with correlation coefficients ≥ 0.9950. The mean recoveries for most of the pesticides were in the range of 70%−120% with RSD < 20%. The limits of detection ranged 0.28–2.00 μg/kg, while the limits of quantification were 0.94–6.65 μg/kg. Following the application of "top-down" approach, the expanded measurement uncertainty for all the analytes was < 30%. The proposed method was successfully applied to determine pesticide residues in 20 market samples in China, where 9 pesticides were detected and quintozene exceeded the criteria domestically and abroad.

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Simultaneous determination of 74 pesticide residues in Panax notoginseng by QuEChERS coupled with gas chromatography tandem mass spectrometry

Show Author's information Hide Author's Information Huijun Li^{^a^,^b^,^d}, Jieshan Wu^{^c}, Chao Chen^{^a^,^b^,^d}, Wenfeng Xin^{^e}, Wensheng Zhang^{^a^,^b^,^d}(

)

Engineering Research Center of Natural Medicine, Ministry of Education, Beijing Normal University at Zhuhai 519087, China

Zhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University at Zhuhai 519087, China

Gongbei customs of the People's Republic of China, Zhuhai 519000, China

Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China

National and Local United Engineering Research Center for Panax Notoginseng Resources Protection and Utilization Technology, Kunming 650000, China

Abstract

Keywords: Panax notoginseng, QuEChERS, GC-MS/MS, Pesticide residues

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Publication history

Received: 21 May 2020

Revised: 27 June 2020

Accepted: 27 June 2020

Published: 22 March 2021

Issue date: March 2021

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Acknowledgment

This work was supported by the National Key Research and Development Plan of China (2017 YFC1702500).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).