Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor

Triazine herbicides have been widely used in agriculture, but their residues can harm the environment and human health. To help monitor these, we have developed an effective immunochromatographic strip test that can simultaneously detect 15 different triazines in grain samples (including ametryn, cyprazine, atraton, prometon, prometryn, atrazine, propazine, terbuthylazine, simetryn, trietazine, secbumeton, simazine, desmetryn, terbumeton and simetone). Based on our optimization procedure, the visual limit of detection (vLOD) for these triazines was found to be 2–10 ng/mL in assay buffer, and 0.02–0.1 mg/kg in grain samples. Four different grain matrices including corn, brown rice, wheat, and sorghum were studied and the test results showed no significant differences between the 15 triazines analyzed using this method. This test is simple, convenient, rapid, and low-cost, and could be an effective tool for primary screening of triazine residues in grain samples.