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Nano Research 2019, 12(2): 289-297 https://doi.org/10.1007/s12274-018-2213-7
Research Article | Issue | Published: 23 October 2018

Random lasing detection of structural transformation and compositions in silk fibroin scaffolds

Show Author's Information SungYeun Yang1,†,,, ,§,  Soocheol Kim1,§,  HyeIn Shin1,  Seung Ho Choi2,‡,  Young L. Kim2,  Chulmin Joo1( ),  WonHyoung Ryu1( )
Department of Mechanical Engineering,Yonsei University,Yonsei-ro 50, Seodaemun-gu, Seoul,03722,Republic of Korea;
Weldon School of Biomedical Engineering,,Purdue University,West Lafayette, Indiana,47907,USA;

† Present address: Center of Bio Microsystems, Korea Institute of Science and Technology (KIST), Hwarang-ro, Seongbuk-gu, Seoul 02792, Republic of Korea

‡ Present address: School of Electrical Engineering, University of Ulsan, Daehak-ro, Nam-gu, Ulsan 44610, Republic of Korea

§ SungYeun Yang and Soocheol Kim contributed equally to this work.

Keywords:
hydroxyapatite, electrospinning, random lasing, silk fibroin, porous scaffolds, aligned pore structure
Topics:
Cell engineeringElectrospinningNanoparticlesPore structureRefractive indexStructure (composition)TissueTissue regeneration
Cite this article:
Yang S, Kim S, Shin H, et al. Random lasing detection of structural transformation and compositions in silk fibroin scaffolds. Nano Research, 2019, 12(2): 289-297. https://doi.org/10.1007/s12274-018-2213-7
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Abstract Full text About this article

In tissue engineering, microstructure and material composition of tissue scaffolds have major influences on the proliferation and differentiation of cells in the scaffolds. However, once tissue scaffolds implanted, it is extremely difficult to monitor the change of their microstructure and compositions during tissue regeneration. Here, we report how random lasing can be utilized to non-invasively monitor the structure and composition of scaffolds. We hypothesize that morphological and compositional change of silk fibroin (SF) scaffolds can be conveniently detected based on random lasing responses. Engineered SF scaffolds with hydroxyapatite (HAP) nanoparticles and controlled pore alignment were fabricated, and their random lasing responses were analyzed depending on the concentration of HAP nanoparticles and the degree of internal pore alignment. We also examined the real-time random lasing responses of porous SF scaffolds by applying a compressive force to the scaffolds. Introduction of HAP nanoparticles lowered the lasing thresholds and narrowed the random lasing (RL) width dramatically, which is likely due to the increase in heterogeneity in both refractive index and physical arrangement within the SF and HAP composites. The strong dependency of RL response on pore alignment was also measured and validated by numerical calculation with the finite element method (FEM). Finally, real-time monitoring of RL on compressed scaffolds demonstrated the possibility of using RL as a monitoring tool for structural change of SF scaffolds in vivo.


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About this article

Random lasing detection of structural transformation and compositions in silk fibroin scaffolds

Show Author's information SungYeun Yang1,†,,, ,§, Soocheol Kim1,§, HyeIn Shin1, Seung Ho Choi2,‡, Young L. Kim2, Chulmin Joo1( ), WonHyoung Ryu1( )
Department of Mechanical Engineering,Yonsei University,Yonsei-ro 50, Seodaemun-gu, Seoul,03722,Republic of Korea;
Weldon School of Biomedical Engineering,,Purdue University,West Lafayette, Indiana,47907,USA;

† Present address: Center of Bio Microsystems, Korea Institute of Science and Technology (KIST), Hwarang-ro, Seongbuk-gu, Seoul 02792, Republic of Korea

‡ Present address: School of Electrical Engineering, University of Ulsan, Daehak-ro, Nam-gu, Ulsan 44610, Republic of Korea

§ SungYeun Yang and Soocheol Kim contributed equally to this work.

Abstract

In tissue engineering, microstructure and material composition of tissue scaffolds have major influences on the proliferation and differentiation of cells in the scaffolds. However, once tissue scaffolds implanted, it is extremely difficult to monitor the change of their microstructure and compositions during tissue regeneration. Here, we report how random lasing can be utilized to non-invasively monitor the structure and composition of scaffolds. We hypothesize that morphological and compositional change of silk fibroin (SF) scaffolds can be conveniently detected based on random lasing responses. Engineered SF scaffolds with hydroxyapatite (HAP) nanoparticles and controlled pore alignment were fabricated, and their random lasing responses were analyzed depending on the concentration of HAP nanoparticles and the degree of internal pore alignment. We also examined the real-time random lasing responses of porous SF scaffolds by applying a compressive force to the scaffolds. Introduction of HAP nanoparticles lowered the lasing thresholds and narrowed the random lasing (RL) width dramatically, which is likely due to the increase in heterogeneity in both refractive index and physical arrangement within the SF and HAP composites. The strong dependency of RL response on pore alignment was also measured and validated by numerical calculation with the finite element method (FEM). Finally, real-time monitoring of RL on compressed scaffolds demonstrated the possibility of using RL as a monitoring tool for structural change of SF scaffolds in vivo.

Keywords: hydroxyapatite, electrospinning, random lasing, silk fibroin, porous scaffolds, aligned pore structure

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Publication history
Copyright
Acknowledgements

Publication history

Received: 11 June 2018
Revised: 21 September 2018
Accepted: 25 September 2018
Published: 23 October 2018
Issue date: February 2019

Copyright

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Acknowledgements

Acknowledgements

This research was supported by the research program of the National Research Foundation of Korea (NRF) (NRF-2015R1A5A1037668).

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