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Nano Research 2017, 10(3): 776-784 https://doi.org/10.1007/s12274-016-1313-5
Research Article | Issue | Published: 26 November 2016

A titanium dioxide nanorod array as a high-affinity nano-bio interface of a microfluidic device for efficient capture of circulating tumor cells

Show Author's Information Jichuan Qiu1,2,§,  Kun Zhao1,§,  Linlin Li1,§,  Xin Yu1,  Weibo Guo1,  Shu Wang1,  Xiaodi Zhang1,  Caofeng Pan1( ),  Zhong Lin Wang1( ),  Hong Liu2( )
Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences; National Center for Nanoscience and Technology (NCNST) Beijing 100083 China
Bio-Micro-Nano Functional Materials Center State Key Laboratory of Crystal Materials Shandong University Jinan 250100 China

§ These authors contributed equally to this work.

Keywords:
nano-bio interface, TiO2 nanorod array, circulating tumor cell, microfluidic device, 3D hierarchical structure
Cite this article:
Qiu J, Zhao K, Li L, et al. A titanium dioxide nanorod array as a high-affinity nano-bio interface of a microfluidic device for efficient capture of circulating tumor cells. Nano Research, 2017, 10(3): 776-784. https://doi.org/10.1007/s12274-016-1313-5
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Abstract Full text Electronic supplementary material About this article

Nanomaterials show promising opportunities to address clinical problems (such as insufficient capture of circulating tumor cells; CTCs) via the high surface area-to-volume ratio and high affinity for biological cells. However, how to apply these nanomaterials as a nano-bio interface in a microfluidic device for efficient CTC capture with high specificity remains a challenge. In the present work, we first found that a titanium dioxide (TiO2) nanorod array that can be conveniently prepared on multiple kinds of substrates has high affinity for tumor cells. Then, the TiO2 nanorod array was vertically grown on the surface of a microchannel with hexagonally patterned Si micropillars via a hydrothermal reaction, forming a new kind of a micro-nano 3D hierarchically structured microfluidic device. The vertically grown TiO2 nanorod array was used as a sensitive nano-bio interface of this 3D hierarchically structured microfluidic device, which showed high efficiency of CTC capture (76.7% ± 7.1%) in an artificial whole-blood sample.


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About this article

A titanium dioxide nanorod array as a high-affinity nano-bio interface of a microfluidic device for efficient capture of circulating tumor cells

Show Author's information Jichuan Qiu1,2,§, Kun Zhao1,§, Linlin Li1,§, Xin Yu1, Weibo Guo1, Shu Wang1, Xiaodi Zhang1, Caofeng Pan1( ), Zhong Lin Wang1( ), Hong Liu2( )
Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences; National Center for Nanoscience and Technology (NCNST) Beijing 100083 China
Bio-Micro-Nano Functional Materials Center State Key Laboratory of Crystal Materials Shandong University Jinan 250100 China

§ These authors contributed equally to this work.

Abstract

Nanomaterials show promising opportunities to address clinical problems (such as insufficient capture of circulating tumor cells; CTCs) via the high surface area-to-volume ratio and high affinity for biological cells. However, how to apply these nanomaterials as a nano-bio interface in a microfluidic device for efficient CTC capture with high specificity remains a challenge. In the present work, we first found that a titanium dioxide (TiO2) nanorod array that can be conveniently prepared on multiple kinds of substrates has high affinity for tumor cells. Then, the TiO2 nanorod array was vertically grown on the surface of a microchannel with hexagonally patterned Si micropillars via a hydrothermal reaction, forming a new kind of a micro-nano 3D hierarchically structured microfluidic device. The vertically grown TiO2 nanorod array was used as a sensitive nano-bio interface of this 3D hierarchically structured microfluidic device, which showed high efficiency of CTC capture (76.7% ± 7.1%) in an artificial whole-blood sample.

Keywords: nano-bio interface, TiO2 nanorod array, circulating tumor cell, microfluidic device, 3D hierarchical structure

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Publication history
Copyright
Acknowledgements

Publication history

Received: 05 August 2016
Revised: 23 September 2016
Accepted: 01 October 2016
Published: 26 November 2016
Issue date: March 2017

Copyright

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016

Acknowledgements

Acknowledgements

The authors are thankful for funding from the National Natural Science Foundation of China (Nos. 51402063, 51432005, 61405040, 61505010, 51502018, 31270022, and 81471784), the "100 Talents Program" of the Chinese Academy of Sciences, Beijing City Committee of science and technology (No. Z151100003315010), Beijing Natural Science Foundation (Nos. 2164077 and 2164076), the Fundamental Research Funds of Shandong University (No. 2014QY003), and the Youth Innovation Promotion Association of the Chinese Academy of Sciences (No. 2015023). The authors also acknowledge the support from the "thousands talents" program for pioneer researchers and his innovation team, and support from the President Funding of the Chinese Academy of Sciences.

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