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Publishing Language: Chinese | Open Access

Tailored Aptamer-Based Visual Colorimetric Detection of Staphylococcal Enterotoxin A Using Gold Nanoparticles

Liwei CUI1 , Rong WEI1, Weidan CHANG1, Xiaoyu YUE1, Wentao XU1,2 ( )
School of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health, China Agricultural University, Beijing 100191, China
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Abstract

Objective

To select and use aptamers as molecular recognition elements to construct a biosensor based on the salt effect of gold nanoparticles for the rapid quantitative detection of staphylococcal enterotoxin A (SEA).

Methods

Molecular docking and molecular dynamics simulation were used to truncate the SEA aptamer and predict the results. The simulation results were verified colorimetrically using gold nanoparticles. The optimal SEA aptamer was used as the molecular recognition element. The reaction system was optimized, the relationship between SEA mass concentration and absorbance ratio was explored, and the accuracy, precision and specificity of the developed method were evaluated.

Results

An SEA aptamer with short sequence, high affinity, strong specificity and stability was selected. This method was characterized by low limit of detection (LOD) and satisfactory recoveries for spiked samples.

Conclusion

Molecular simulation can effectively improve the screening efficiency of aptamers, and the proposed method can be used for rapid detection of SEA.

Keywords

staphylococcal enterotoxin Avisualizationaptamertruncationmolecular dynamics simulationgold nanoparticles-based colorimetry
CLC number: TS207.3 Document code: A Article ID: 1002-6630(2023)24-0323-06

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Food Science
Volume 44 Issue 24,
December 2023
Pages 323-328
DOI: 10.7506/spkx1002-6630-20230404-029

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Cite this article:
CUI L, WEI R, CHANG W, et al. Tailored Aptamer-Based Visual Colorimetric Detection of Staphylococcal Enterotoxin A Using Gold Nanoparticles. Food Science, 2023, 44(24): 323-328. https://doi.org/10.7506/spkx1002-6630-20230404-029

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Received: 04 April 2023
Published: 25 December 2023
© Beijing Academy of Food Sciences 2023.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).