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Publishing Language: Chinese | Open Access

Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae

Jianju HE1 , Xinxin LIU1, Hongling SHI1, Luni RAN1, Xian WANG2, Yingjun ZHANG1, Cunduo TANG1,2,3 ( )
College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China
Postdoctoral Innovation Practice Base, She Dian Lao Jiu Co. Ltd., Nanyang 473300, China
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
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Abstract

In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was 2252.59 IU/mg. In addition, the kinetic parameters of ScNRK1 were significantly higher than those of other reported nicotinamide nucleoside kinases, so ScNRK1 could be more advantageous in the enzymatic synthesis of nicotinamide mononucleotide (NMN).

Keywords

nicotinamide nucleoside kinaseβ-nicotinamide mononucleotideexpressionidentificationbiocatalysis
CLC number: Q814.9 Document code: A Article ID: 1002-6630(2023)16-0192-06

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Food Science
Volume 44 Issue 16,
August 2023
Pages 192-197
DOI: 10.7506/spkx1002-6630-20220912-095

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Cite this article:
HE J, LIU X, SHI H, et al. Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae. Food Science, 2023, 44(16): 192-197. https://doi.org/10.7506/spkx1002-6630-20220912-095

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Received: 12 September 2022
Published: 25 August 2023
© Beijing Academy of Food Sciences 2023.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).