Development of a Dual-Probe Competitive Enzyme-Linked Immunosorbent Assay Based on Magnetic Nanoparticles for the Detection of Fumonisin B

In this study, a dual-probe competitive enzyme-linked immunosorbent assay (ELISA) based on magnetic nanoparticles for the simultaneous detection of fumonisin B₁ (FB₁), FB₂ and FB₃ in maize samples was established. A magnetic capture probe (MNPs-FB₁) was synthesized by the active ester method, and a monoclonal antibody-horseradish peroxidase (McAb-HRP) probe was prepared by a modified sodium periodate method. The reaction conditions of the assay were optimized. The linear ranges for FB₁ and the total amount of FB₁, FB₂ and FB₃ (FBs) were 0.07–1.98 and 0.10–9.86 ng/mL, respectively. The average recoveries of FBs from spiked maize samples ranged from 86.2% to 105.1%. The correlation coefficient between the results of the developed assay and those of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was 0.9966. These results suggest that this assay is characterized by rapidity, high accuracy and good repeatability and provides a new method for the detection of FBs in maize samples.