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Publishing Language: Chinese | Open Access

Preparation and Characterization of Bovine Bone Peptide-Calcium Chelate and Its Promoting Effect on Osteogenic Activity of MC3T3-E1 Cells

Liwei QI1 , Hongru ZHANG1, Yujie GUO1 ( ), Hong LIU1, Ruilin LI2, Chunhui ZHANG1 ( ), Yang XU3
Comprehensive Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
School of Food and Wine, Ningxia University, Yinchuan 750021, China
Inner Mongolia Mengtai Biological Engineering Co. Ltd., Hohhot 011517, China
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Abstract

Purpose

To prepare a calcium supplement for efficient absorption and utilization in the human body and to explore its effect on the osteogenic activity of MC3T3-E1 cells.

Methods

Bovine bone peptide was chelated with calcium, and the optimal reaction conditions were determined by response surface methodology (RSM). The structural characteristics and stability of peptide-calcium chelate were studied by infrared spectroscopy, X-ray diffraction and atomic absorption spectrometry. The effects of bovine bone peptide-calcium chelate on the proliferation, differentiation and mineralization of MC3T3-E1 cells were investigated.

Results

The optimum conditions for the preparation of bovine bone peptide calcium chelate were peptide/calcium mass ratio 2.97, temperature 62.54 ℃ and pH 9.06. Under these conditions, the calcium chelating capacity was 55.65 μg/mg. The results of spectral analysis showed that amino nitrogen, hydroxyl oxygen and carboxyl oxygen were the major calcium-chelating sites. Bovine bone peptide could form coordinate bonds with calcium, and the molecular mass of the peptide increased after chelation with calcium. In addition, the stability of bovine bone peptide-calcium chelate was little affected by temperature, but sensitive to pH. The peptide-calcium chelate could significantly promote the proliferation, differentiation and mineralization of MC3T3-E1 cells.

Conclusion

Bovine bone peptide-calcium chelate has the potential to promote the osteogenic activity of MC3T3-E1 cells.

Keywords

bovine bone peptide-calcium chelatestructural characterizationMC3T3-E1 cellsosteogenic activity
CLC number: TS251.94 Document code: A Article ID: 1002-6630(2023)06-0107-09

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Food Science
Volume 44 Issue 6,
March 2023
Pages 107-115
DOI: 10.7506/spkx1002-6630-20220319-226

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Cite this article:
QI L, ZHANG H, GUO Y, et al. Preparation and Characterization of Bovine Bone Peptide-Calcium Chelate and Its Promoting Effect on Osteogenic Activity of MC3T3-E1 Cells. Food Science, 2023, 44(6): 107-115. https://doi.org/10.7506/spkx1002-6630-20220319-226

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Received: 19 March 2022
Published: 25 March 2023
© Beijing Academy of Food Sciences 2023.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).