A Microdroplet Digital PCR Assay for Quantitative Analysis of Goat Milk Powder

Driven by the urgent market demand for milk traceability technologies, a microdroplet digital polymerase chain reaction (PCR) assay was developed to accurately identify the milk source in goat milk powder. This method overcame the shortcomings of complex ingredients and long time required for identifying the source of milk, and could detect it more accurately and reduce the possibility of misjudgment. In this study, a model equation for describing the relationship between the number of DNA copies per μL from goat milk powder (C) and its mass (M, mg) was proposed as follows: M = (C−4.75)/3.56.