Effect of the Inhibition of PDIDSM_85260 and PDIDSM_08010 Expression by RNA Interference on the Biotransformation of Limonene to α-Terpineol

Our previous results have shown that some genes such as PDIDSM_85260 and PDIDSM_08010 may be partly responsible for limonene biotransformation to α-terpineol as determined by analysis of genomic and transcriptomic data, protein separation and purification, and mass spectrometry. In order to explore the biological functions of the genes, RNA interference (RNAi) vectors for PDIDSM_85260 and PDIDSM_08010 were constructed and transformed into Penicillium digitatum DSM62840 by Agrobacterium tumefaciens-mediated transformation. RANi strains were obtained by a series of experiments, and the functions of the two genes were analyzed in P. digitatum DSM62840. It was found that the amount of α-terpineol produced by the silenced transformants obviously decreased compared to the wild-type strain, suggesting that PDIDSM_85260 and PDIDSM_08010 may participate in limonene biotransformation to α-terpineol.