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Publishing Language: Chinese | Open Access

Isolation, Purification and Mass Spectrometry Identification of Enzyme Involved in Limonene Biotransformation into α-Terpineol by Penicillium digitatum DSM62840

Lulu ZHANG1 , Gang FAN2 ( ), Xiao LI2, Jingnan REN2, Siyi PAN2, Wen HUANG2, Jin HE3
College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China
Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
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Abstract

The key enzyme involved in the biotransformation of limonene into α-terpineol by Penicillium digitatum DSM62840 was extracted and purified by sequential ultracentrifugation, ammonium sulfate precipitation, hydroxyapatite chromatography and gel filtration chromatography after high pressure disruption of cells. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed an obvious protein band with a molecular mass of approximatelty 50 kDa. This protein was tentatively identified as dihydrolipoyl dehydrogenase (PDIDSM_08010) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results of this study may provide a basis and reference for α-terpineol production.

Keywords

Penicillium digitatum DSM62840limonene biotransformationα-terpineolseparation and purificationmass spectrometry
CLC number: TS201.3 Document code: A Article ID: 1002-6630(2022)04-0088-07

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Food Science
Volume 43 Issue 4,
February 2022
Pages 88-94
DOI: 10.7506/spkx1002-6630-20210113-145

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Cite this article:
ZHANG L, FAN G, LI X, et al. Isolation, Purification and Mass Spectrometry Identification of Enzyme Involved in Limonene Biotransformation into α-Terpineol by Penicillium digitatum DSM62840. Food Science, 2022, 43(4): 88-94. https://doi.org/10.7506/spkx1002-6630-20210113-145

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Received: 13 January 2021
Published: 25 February 2022
© Beijing Academy of Food Sciences 2022.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).