Influence of Chlorogenic Acid from Lycium barbarum on Cardiac Fibroblast Fibrosis Induced by Transforming Growth Factor-β1

To investigate the protective effect of chlorogenic acid from Lycium barbarum on cardiac fibroblast (CF) fibrosis induced by transforming growth factor-β1 (TGF-β1).

A TGF-β1 concentration of 10 ng/mL was used to induce CF fibrosis. The CFs were further treated with different concentrations of chlorogenic acid, and their proliferation was detected by cell counting kit-8 (CCK-8) assay. The expression of the fibrosis markers collagen (Col) Ⅰ and Col Ⅲ was detected by enzyme linked immunosorbent assay (ELISA) and α-smooth muscle actin (α-SMA) by immunofluorescence. The mRNA expression levels of Acta2, Twist1, Snail1, Snail2, Smad4 and S100A4 were detected by quantitative real-time polymerase chain reaction (qPCR). Western blot analysis was used to evaluate the expression levels TGF-β1/Smads signaling pathway-related proteins and their phosphorylation levels.

Chlorogenic acid from Lycium barbarum could inhibit abnormal cell proliferation, and down-regulate the expression of Col Ⅰ, Col Ⅲ and α-SMA, the mRNA expression of Acta2, Twist1, Snail1, Snail2, Smad4 and S100A4 (P < 0.001) and the expression of the TGF-β1/Smads signaling pathway-related proteins (P < 0.001).

Within the concentration range tested in this study, chlorogenic acid from Lycium barbarum inhibits the fibrosis process of CFs induced by TGF-β1, and the mechanism may be related to the TGF-β1/Smads signaling pathway.