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Article | Open Access

Hepatocarcinoma Single Cell Migration on Micropatterned PDMS Substrates

Bin Zheng1, Shu-Ling Hsieh2, Chih-Chung Wu3, Chun-Hsin Wu4, Pei-Ying Lin5, Chiung-Wen Hsieh5, I-Tin Li5, Yun-Shan Huang6, Huay-Min Wang7, Shuchen Hsieh5( )
School of Materials Science and Engineering, Dalian University of Technology, Dalian, Liaoning 116024, People’s Republic of China
Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 81157, Taiwan
Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, 71101 Taiwan
Department of Computer Science and Information Engineering, National University of Kaohsiung, Kaohsiung, Taiwan
Department of Chemistry, Center for Nanoscience and Nanotechnology, National Sun Yat-sen University Kaohsiung, Taiwan
Department of Food Science and Technology, Tajen University, Pingtung, 90741,Taiwan
Kaohsiung Veterans General Hospital, Kaohsiung, 81362, Taiwan
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Abstract

Cell migration influences many normal and pathological processes and is one of key issues addressed in cancer research studies. In this report, a plasma patterned polydimethylsiloxane (PDMS) substrate was used to selectively position hepatocarcinoma cells in order to characterize their migration behavior. We observed that cell mobility was directly related to the differentiation stage of the cells, with poorly-differentiated (SK-Hep-1) cells exhibiting higher mobility that well-differentiated (Hep-G2) cells. We propose that this difference occurs due to a loss of adhesion molecules presented at the apical membranes of the poorly-differentiated SK-Hep-1 cells, thereby reducing their adhesion to the surface. Our results provide new insight into the relationship between carcinoma cell differentiation grade and mobility. Further this experimental process may provide a simple and effective model for universal cell biology studies and applications in microsystems technology.

Keywords

Cell adhesionCell spreadingHepatocyteMicropatterningPolydimethylsiloxane

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Nano Biomedicine and Engineering
Volume 3 Issue 2,
June 2011
Pages 99-106
DOI: 10.5101/nbe.v3i2.p99-106

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Cite this article:
Zheng B, Hsieh S-L, Wu C-C, et al. Hepatocarcinoma Single Cell Migration on Micropatterned PDMS Substrates. Nano Biomedicine and Engineering, 2011, 3(2): 99-106. https://doi.org/10.5101/nbe.v3i2.p99-106

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Published: 30 June 2011
© 2011 B. Zheng, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.