AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Nano Biomedicine and Engineering Article
PDF (788.5 KB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Article | Open Access

Synergetic Antibacterial Effects of Silver Nanoparticles@Aloe Vera Prepared via a Green Method

Yixia Zhang( ), Dapeng Yang( ), Yifei Kong, Xiansong Wang, Omar Pandoli, Guo Gao
Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P.R. China
Show Author Information

Abstract

Aloe Vera-conjugated Ag nanoparticles (AgNPs@AV hybrids) are synthesized in large quantities by reducing silver nitrate with Aloe Vera pulp extract at room temperature. TEM image reveals that these NPs are predominantly spherical with an average of 25 nm in diameter. The crystal structure of AgNPs@AV is determined by XRD. The cytotoxicity of AgNPs@AV hybrids is detected by carrying out the cell viability measurement on Human Dermal Fibroblasts (HDF) cells, the results show that no obvious cytotoxicity is observed. Compared with Vera gel and Ag NPs (washed from Vera gel) alone, AgNPs@AV hybrids possess more excellent antibacterial activity on E. coli even at very low concentration.

Keywords

CytotoxicityGreen synthesisSynergetic effectSilver NPsAloe Vera

References

[1]

Yang, D.; Cui, D., Advances and prospects of gold nanorods. Chem.- Asian J. 2008, 3 (12), 2010-2022. doi:10.1002/asia.200800195
Crossref Google Scholar
[2]

Panyam, J.; Labhasetwar, V., Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv. Drug Delivery Rev. 2003, 55 (3), 329-347. doi:10.1016/S0169-409X(02)00228-4
Crossref Google Scholar
[3]

Park, J.; Gu, L.; von Maltzahn, G.; Ruoslahti, E.; Bhatia, S.; Sailor, M., Biodegradable luminescent porous silicon nanoparticles for in vivo applications. Nat. Mater. 2009, 8 (4), 331-336. doi:10.1038/nmat2398
Crossref Google Scholar
[4]

Uludag, Y.; Tothill, I., Development of a Sensitive Detection method of Cancer Biomarkers in Human Serum (75%) using a Quartz Crystal Microbalance Sensor and Nanoparticles amplification system. Talanta 2010, 82, 277-282. doi:10.1016/j.talanta.2010.04.034
Crossref Google Scholar
[5]

Sanpui, P.; Murugadoss, A.; Prasad, P.; Ghosh, S.; Chattopadhyay, A., The antibacterial properties of a novel chitosan-Ag-nanoparticle composite. Int. J. Food Microbiol. 2008, 124 (2), 142-146. doi:10.1016/j.ijfoodmicro.2008.03.004
Crossref Google Scholar
[6]

Cho, K.; Park, J.; Osaka, T.; Park, S., The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim. Acta 2005, 51 (5), 956-960. doi:10.1016/j.electacta.2005.04.071
Crossref Google Scholar
[7]

Kvitek, L.; Panac ek, A.; Soukupova, J.; Kolar, M.; Vec erova, R.; Prucek, R.; Holecova, M.; Zboril, R., Effect of surfactants and polymers on stability and antibacterial activity of silver nanoparticles (NPs). J. Phys. Chem. C 2008, 112 (15), 5825-5834. doi:10.1021/jp711616v
Crossref Google Scholar
[8]

Stoimenov, P.; Klinger, R.; Marchin, G.; Klabunde, K., Metal oxide nanoparticles as bactericidal agents. Langmuir 2002, 18 (17), 6679-6686. doi:10.1021/la0202374
Crossref Google Scholar
[9]

Xie, J.; Lee, J.; Wang, D.; Ting, Y., Silver nanoplates: from biological to biomimetic synthesis. ACS nano 2007, 1 (5), 429-439. doi:10.1021/nn7000883
Crossref Google Scholar
[10]

N, M.; Nadagouda; S, R.; Varma, Green synthesis of silver and palladium nanoparticles at room temperature using coffee and tea extract. Green Chem. 2008, 10 (8), 859-862. doi:10.1039/b804703k
Crossref Google Scholar
[11]

Yang, D.; Chen, S.; Huang, P.; Wang, X.; Jiang, W.; Pandoli, O.; Cui, D., Bacteria-template synthesized silver microspheres with hollow and porous structures as excellent SERS substrate. Green Chem. 2010, 12, 2038-2042. doi:10.1039/c0gc00431f
Crossref Google Scholar
[12]

Huang P, Kong Y, Li Z, Gao F, Cui D. Copper selenide nanosnakes: bovine serum albumin-assisted room temperature controllable synthesis and characterization. Nanoscale Res. Lett. 2010, 5(6), 949-956. doi:10.1007/s11671-010-9587-0.
Crossref Google Scholar
[13]

Huang P, Li Z, Hu H, Cui D. Synthesis and Characterization of Bovine Serum Albumin-conjugated Copper Sulfide Nanocomposites. J. Nanomater. 2010, doi:10.1155/2010/641545.
Crossref Google Scholar
[14]
Huang P, Bao L, Yang D, Gao G, Lin J, Li Z, et al. Protein-directed solution-phase green synthesis of BSA-conjugated MxSey (M=Ag, Cd, Pb, Cu) nanomaterials. Chem Asian J. In press.
[15]

Shankar, S.; Ahmad, A.; Pasricha, R.; Sastry, M., Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J. Mater. Chem. 2003, 13 (7), 1822-1826. doi:10.1039/b303808b
Crossref Google Scholar
[16]

Mukherjee, P.; Ahmad, A.; Mandal, D.; Senapati, S.; Sainkar, S.; Khan, M.; Parishcha, R.; Ajaykumar, P.; Alam, M.; Kumar, R., Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis. Nano Lett. 2001, 1 (10), 515-519. doi:10.1021/nl0155274
Crossref Google Scholar
[17]

Kong, Y.; Chen, J.; Gao, F.; Li, W.; Xu, X.; Pandoli, O.; Yang, H.; Ji, J.; Cui, D., A Multifunctional Ribonuclease©\A©\Conjugated CdTe Quantum Dot Cluster Nanosystem for Synchronous Cancer Imaging and Therapy. Small 2010, 6 (21), 2367–2373. doi:10.1002/smll.201001050
Crossref Google Scholar
[18]

Kong, Y.; Gao, F.; He, R.; Chen, J.; Xu, X.; Li, N.; Cui, D., Bio-inspired synthesis of bovine serum albumin conjugated Ag2Se/Se core/shell heterostructure nanoparticles at room temperature. Curr. Nanosci. 2010, 6, 446.
Crossref Google Scholar
[19]

Shankar, S.; Ahmad, A.; Sastry, M., Geranium leaf assisted bio-synthesis of silver nanoparticles. Biotechnol. Prog. 2003, 19 (6), 1627-1631. doi:10.1021/bp034070w
Crossref Google Scholar
[20]

Zawahry, M.; Hegazy, M.; Helal, M., Use of aloe iim treating leg ulcers and dermatoses. Int. J. Dermatol. 1973, 12 (1), 68-73. doi:10.1111/j.1365-4362.1973.tb00215.x
Crossref Google Scholar
[21]

Vogler, B.; Ernst, E., Aloe vera: a systematic review of its clinical effectiveness. Br. J. General Pract. 1999, 49 (447), 823.
Google Scholar
[22]

Agarry, O.; Olaleye, M.; Bello-Michael, C., Comparative antimicrobial activities of aloe vera gel and leaf. Afr. J. Biotechnol. 2005, 4 (12), 1413-1414.
Google Scholar
[23]
CA, N.; LA, A.; D, P. J., Herbal Medicines: A guide for health-care for health-care professionals. 1996, 296.
[24]

Davis, R.; Donato, J.; Hartman, G.; Haas, R., Anti-inflammatory and wound healing activity of a growth substance in Aloe vera. J. Am. Podiatr. Med. Assoc. 1994, 84 (2), 77-81.
Crossref Google Scholar
[25]

Vazquez, B.; Avila, G.; Segura, D.; Escalante, B., Antiinflammatory activity of extracts from Aloe Vera gel. J. Ethnopharmacol. 1996, 55, 69-75. doi:10.1016/S0378-8741(96)01476-6
Crossref Google Scholar
[26]

Feng, Q.; Wu, J.; Chen, G.; Cui, F.; Kim, T.; Kim, J., A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J. Biomed. Mater. Res., Part A 2000, 52 (4), 662-668. doi:10.1002/1097-4636(20001215)52:4<662::AIDJBM10>3.0.CO;2-3
Crossref Google Scholar
[27]

Chandran, S.; Chaudhary, M.; Pasricha, R.; Ahmad, A.; Sastry, M., Synthesis of gold nanotriangles and silver nanoparticles using aloevera plant extract. Biotechnol. Prog. 2006, 22 (2), 577-583. doi:10.1021/bp0501423
Crossref Google Scholar
[28]

Philip, D., Biosynthesis of Au, Ag and Au-Ag nanoparticles using edible mushroom extract. Spectrochim. Acta, Part A 2009, 73 (2), 374-381. doi:10.1016/j.saa.2009.02.037
Crossref Google Scholar
[29]

Kannan, P.; John, S., Synthesis of mercaptothiadiazole-nctionalized gold nanoparticles and their self-assembly on Au substrates. Nanotechnology 2008, 19, 085602. doi:10.1088/0957-484/19/8/085602
Crossref Google Scholar
[30]

Hart, L.; Van den Berg, A.; Kuis, L.; Van Dijk, H.; Labadie, R., An anti-complementary polysaccharide with immunological adjuvant activity from the leaf parenchyma gel of Aloe vera. Planta Med. 1989, 55 (6), 509-512.
Crossref Google Scholar
[31]

Marambio-Jones, C.; Hoek, E., A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J. Nanopart. Res. 2010, 12 (5), 1531-1551. doi:10.1007/s11051-010-9900-y
Crossref Google Scholar
[32]

Pal, S.; Tak, Y.; Song, J., Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl. Environ. microbiol. 2007, 73 (6), 1712–1720. doi:10.1128/AEM.02218-06
Crossref Google Scholar
[33]

Fly, L.; Kiem, I., Tests of Aloe vera for antibiotic activity. Economic Botany 1963, 17 (1), 46-49. doi:10.1007/BF02985352
Crossref Google Scholar
[34]

Paes-Leme, A.; Motta, E.; De Mattos, J.; Dantas, F.; Bezerra, R.; Caldeira-de-Araujo, A., Assessment of Aloe vera (L.) genotoxic potential on Escherichia coli and plasmid DNA. J. Ethnopharmacol. 2005, 102 (2), 197-201. doi:10.1016/j.jep.2005.06.013
Crossref Google Scholar
[35]

Gogoi, S.; Gopinath, P.; Paul, A.; Ramesh, A.; Ghosh, S.; Chattopadhyay, A., Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir 2006, 22 (22), 9322-9328. doi:10.1021/la060661v
Crossref Google Scholar
[36]

Morones, J. R.; Elechiguerra, J. L.; Camacho, A.; Holt, K.; Juan B Kouri4, The bactericidal effect of silver nanoparticles. Nanotechnology 2005, 16 (10), 2346–2353. doi:10.1088/0957-448 4/16/10/059
Crossref Google Scholar
Nano Biomedicine and Engineering
Volume 2 Issue 4,
December 2010
Pages 252-257
DOI: 10.5101/nbe.v2i4.p252-257
Cite this article:
Zhang Y, Yang D, Kong Y, et al. Synergetic Antibacterial Effects of Silver Nanoparticles@Aloe Vera Prepared via a Green Method. Nano Biomedicine and Engineering, 2010, 2(4): 252-257. https://doi.org/10.5101/nbe.v2i4.p252-257

612

Views

16

Downloads

49

Crossref

86

Scopus

Altmetrics
Received: 15 November 2010
Accepted: 06 December 2010
Published: 16 December 2010
© 2010 Y. Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Return