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Nano Biomedicine and Engineering 2022, 14(4): 295-307 https://doi.org/10.5101/nbe.v14i4.p295-307
Research Article | Open Access | Issue | Published: 31 December 2022

Green Synthesis, Characterization and Antiepileptic Activity of Herbal Nanoparticles of Mimusops Elengi in Mice

Show Author's Information Jayaraman Rajangam1( ),  Pushpalatha Sampathi2,  Narahari Narayan Palei1,  Anna Balaji2,  Shyam Sundar A.3,  Bibhash Chandra Mohanta4,  Vasanth Raj Palanimuthu5
Amity Institue of Pharmacy, Amity University, Lucknow, India
Department of Pharmacology, Sree Vidyanikethan College of Pharmacy, Tirupati, India
College of Pharmacy and Nursing, University of Nizwa, Nizwa, Oman
College of Pharmacy, Teerthanker Mahaveer University, Uttar Pradesh, India
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy Ooty, Tamil Nadu, India
Keywords:
Green synthesis, Acetylcholinesterase, Antioxidants, Mimusops elengi, Herbal nanoparticles, Electro convulsions, Chemo convulsions
Cite this article:
Rajangam J, Sampathi P, Palei NN, et al. Green Synthesis, Characterization and Antiepileptic Activity of Herbal Nanoparticles of Mimusops Elengi in Mice. Nano Biomedicine and Engineering, 2022, 14(4): 295-307. https://doi.org/10.5101/nbe.v14i4.p295-307
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Abstract Full text About this article

The aim of the study was to green synthesis and evaluate the antiepileptic activity of herbal nanoparticles of Mimusops elengi (ME) on electrochemical convulsion models in mice. ME herbal nanoparticles (MEHNPs) were synthesized and characterized. The particles size and zeta potential of MEHNPs were found to be 24 nm and 29.5 mV respectively. FTIR study revealed that minor shifts of peaks may be due to capping, reduction and stabilization of MEHNPs. Electro convulsions (ECs) were induced using electro-convulsiometer and ear-clip electrodes with alternating current of 45 mA for 0.2 s stimulation. PTZ was administered intraperitoneally to mice at a dose of 105 mg/kg, which was the CD97 (97% convulsive dose for the clonic phase) for inducing chemo-convulsions. The ability of methanol extract of ME bark (MEME-B) and ME herbal nanoparticles (MEHNPs) to reduce the hind limb extension (HLE) in EC and clonic-type (CT) convulsions in the PTZ model was used to establish antiepileptic parameters. HLE and CC was reduced significantly (P < 0.05) whereas neurochemical analyses show a significant rise in Acetylcholinesterase (AChE) and Gamma Butyric acid (GABA) levels. Antioxidant studies revealed that both MEME and MEHNPs significantly increased antioxidant enzymes and scavenged free radicals (P < 0.05), whereas histological findings revealed the protective effects. In both models, MEME-B and MEHNPs exhibited significant anti-epileptic properties, which could be attributed to suppressing excitatory neurohumoral transmission and enhanced inhibitory neurotransmission, as well as a significant increase in antioxidant status. MEHNPs, on the other hand, have outperformed MEME-B in terms of reducing oxidative stress and altering neurochemicals such as AChE and GABA.


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About this article

Green Synthesis, Characterization and Antiepileptic Activity of Herbal Nanoparticles of Mimusops Elengi in Mice

Show Author's information Jayaraman Rajangam1( ), Pushpalatha Sampathi2, Narahari Narayan Palei1, Anna Balaji2, Shyam Sundar A.3, Bibhash Chandra Mohanta4, Vasanth Raj Palanimuthu5
Amity Institue of Pharmacy, Amity University, Lucknow, India
Department of Pharmacology, Sree Vidyanikethan College of Pharmacy, Tirupati, India
College of Pharmacy and Nursing, University of Nizwa, Nizwa, Oman
College of Pharmacy, Teerthanker Mahaveer University, Uttar Pradesh, India
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy Ooty, Tamil Nadu, India

Abstract

The aim of the study was to green synthesis and evaluate the antiepileptic activity of herbal nanoparticles of Mimusops elengi (ME) on electrochemical convulsion models in mice. ME herbal nanoparticles (MEHNPs) were synthesized and characterized. The particles size and zeta potential of MEHNPs were found to be 24 nm and 29.5 mV respectively. FTIR study revealed that minor shifts of peaks may be due to capping, reduction and stabilization of MEHNPs. Electro convulsions (ECs) were induced using electro-convulsiometer and ear-clip electrodes with alternating current of 45 mA for 0.2 s stimulation. PTZ was administered intraperitoneally to mice at a dose of 105 mg/kg, which was the CD97 (97% convulsive dose for the clonic phase) for inducing chemo-convulsions. The ability of methanol extract of ME bark (MEME-B) and ME herbal nanoparticles (MEHNPs) to reduce the hind limb extension (HLE) in EC and clonic-type (CT) convulsions in the PTZ model was used to establish antiepileptic parameters. HLE and CC was reduced significantly (P < 0.05) whereas neurochemical analyses show a significant rise in Acetylcholinesterase (AChE) and Gamma Butyric acid (GABA) levels. Antioxidant studies revealed that both MEME and MEHNPs significantly increased antioxidant enzymes and scavenged free radicals (P < 0.05), whereas histological findings revealed the protective effects. In both models, MEME-B and MEHNPs exhibited significant anti-epileptic properties, which could be attributed to suppressing excitatory neurohumoral transmission and enhanced inhibitory neurotransmission, as well as a significant increase in antioxidant status. MEHNPs, on the other hand, have outperformed MEME-B in terms of reducing oxidative stress and altering neurochemicals such as AChE and GABA.

Keywords: Green synthesis, Acetylcholinesterase, Antioxidants, Mimusops elengi, Herbal nanoparticles, Electro convulsions, Chemo convulsions

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Received: 23 November 2021
Revised: 01 March 2022
Accepted: 29 December 2022
Published: 31 December 2022
Issue date: December 2022

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© Jayaraman Rajangam, Pushpalatha Sampathi, Narahari Narayan Palei, Anna Balaji, Shyam Sundar A., Bibhash Chandra Mohanta and Vasanth Raj Palanimuthu.

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The authors gratefully thank the management of Sree Vidyanikethan Educational Trust (SVET) for providing the required facilities and support.

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