Synthesis, Characterization and <i>In-Vitro</i> Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles for Biomedical Applications

Sivaranjani Sivalingam; Mahalakshmi Santhanakrishnan; Vijaya Parthasarathy

doi:10.5101/nbe.v14i3.p201-207

Nano Biomedicine and Engineering 2022, 14(3): 201-207 https://doi.org/10.5101/nbe.v14i3.p201-207

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Open Access | Issue | Published: 30 November 2022

Synthesis, Characterization and In-Vitro Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles for Biomedical Applications

Show Author's Information Hide Author's Information Sivaranjani Sivalingam, Mahalakshmi Santhanakrishnan, Vijaya Parthasarathy(

)

Department of Nanoscience and Technology, Bharathiar University, Coimbatore, India

Keywords:

Co-precipitation method, Superparamagnetic iron oxide nanoparticles (SPIONs), Allium cepa test, Hemolysis assay

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Sivalingam S, Santhanakrishnan M, Parthasarathy V. Synthesis, Characterization and In-Vitro Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles for Biomedical Applications. Nano Biomedicine and Engineering, 2022, 14(3): 201-207. https://doi.org/10.5101/nbe.v14i3.p201-207

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Abstract

On the growing clinical demands, superparamagnetic iron oxide nanoparticles (SPIONs) have a vital role due to their infinite physical and chemical properties at the nanoscale. The researchers have started to focus more on the magnetite nanoparticle applications with unique shape and size in various filed such as biomedicine, food and environment to synthesize Fe₃O₄ NP. Here we synthesized SPIONs-Fe₃O₄ NP successfully by co-precipitation technique. The prepared nanoparticles were characterized using suitable analytical tools for structural, morphological, elemental, optical and thermogravimetric analysis. Moreover, the genotoxicity and hemolysis assay test has been carried out to estimate the toxicity of SPIONs-Fe₃O₄ NP at various concentrations. The results found that 25 μg/mL concentration of SPIONs-Fe₃O₄ NP shows good hemocompatibility than other concentrations. The genotoxicity assay was examined in Allium cepa (onion root tips) for chromosomal aberration. Hence, the present study discusses the synthesis characterization, assessing the genotoxicity and hemocompatibility potential for the synthesized Fe₃O₄ NPs.

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Synthesis, Characterization and In-Vitro Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles for Biomedical Applications

Show Author's information Hide Author's Information Sivaranjani Sivalingam, Mahalakshmi Santhanakrishnan, Vijaya Parthasarathy(

)

Department of Nanoscience and Technology, Bharathiar University, Coimbatore, India

Abstract

Keywords: Co-precipitation method, Superparamagnetic iron oxide nanoparticles (SPIONs), Allium cepa test, Hemolysis assay

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Publication history

Received: 11 July 2021

Revised: 20 May 2022

Accepted: 28 November 2022

Published: 30 November 2022

Issue date: September 2022

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Acknowledgements

The authors are grateful to DST-FIST and UGC SAP, New Delhi, India for the instrumentation facilities. Funding was provided by the RUSA 2.0 – BEICH Government of India and Department of Science and Technology (DST) PURSE-Phase-II.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.