Green-based Biosynthesis of Zinc Oxide Nanoparticles Using Clitoria Ternatea Flower Extract and Its Antibacterial Activity

The present study reports the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Clitoria ternatea flower extract and examination of their antibacterial activity. The change in color of the reaction mixture from violet to yellow colour indicated the formation of ZnO NPs. The formation of ZnO NPs was confirmed by the appearance of a maximum absorption peak at 381 nm in the UV-visible spectrum. The XRD pattern corresponding with the JCPDS card for ZnO showed the presence of purecrystalline ZnO NPs. FTIR spectra confirmed the stretching vibrations of C=O, C–O–H, and O–H groups involved in the reduction of ZnO NPs. The size and morphology of the ZnO NPs were confirmed by Transmission Electron Microscopy (TEM). Further particle size was determined by using Dynamic Light Scattering (DLS). Furthermore, the antibacterial activity of ZnO NPs showed significant inhibitory activity against Escherichia coli and Staphylococcus aureus bacteria.