Journal Home > Volume 13 , Issue 4
Nano Biomedicine and Engineering 2021, 13(4): 394-400 https://doi.org/10.5101/nbe.v13i4.p394-400
Research Article | Open Access | Issue | Published: 10 December 2021

Green-based Biosynthesis of Zinc Oxide Nanoparticles Using Clitoria Ternatea Flower Extract and Its Antibacterial Activity

Show Author's Information Selvaraj Prabhu1,2( ),  Daniel Thangadurai Thangaian3,  Palaniswamy Vijai Bharathy4
Department of Research and Development, Bharathiar University, Tamil Nadu, India
Department of Physics, Hindusthan College of Engineering and Technology, Coimbatore, Tamil Nadu, India
Department of Nanoscience and Technology, Sri Ramakrishna Engineering College, Coimbatore, Tamil Nadu, India
Department of Physics, C.B.M College, Coimbatore, Tamil Nadu, India
Keywords:
Characterization, XRD, Antibacterial activity, Biosynthesis, Zinc oxide nanoparticles
Cite this article:
Prabhu S, Thangadurai Thangaian D, Vijai Bharathy P. Green-based Biosynthesis of Zinc Oxide Nanoparticles Using Clitoria Ternatea Flower Extract and Its Antibacterial Activity. Nano Biomedicine and Engineering, 2021, 13(4): 394-400. https://doi.org/10.5101/nbe.v13i4.p394-400
Download citation
EndNote(RIS)
BibTeX

734

Views

156

Downloads

Citations

9

Crossref

N/A

WoS

8

Scopus

N/A

CSCD

Abstract Full text About this article

The present study reports the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Clitoria ternatea flower extract and examination of their antibacterial activity. The change in color of the reaction mixture from violet to yellow colour indicated the formation of ZnO NPs. The formation of ZnO NPs was confirmed by the appearance of a maximum absorption peak at 381 nm in the UV-visible spectrum. The XRD pattern corresponding with the JCPDS card for ZnO showed the presence of purecrystalline ZnO NPs. FTIR spectra confirmed the stretching vibrations of C=O, C–O–H, and O–H groups involved in the reduction of ZnO NPs. The size and morphology of the ZnO NPs were confirmed by Transmission Electron Microscopy (TEM). Further particle size was determined by using Dynamic Light Scattering (DLS). Furthermore, the antibacterial activity of ZnO NPs showed significant inhibitory activity against Escherichia coli and Staphylococcus aureus bacteria.


menu
Abstract
Full text
Outline
About this article

Green-based Biosynthesis of Zinc Oxide Nanoparticles Using Clitoria Ternatea Flower Extract and Its Antibacterial Activity

Show Author's information Selvaraj Prabhu1,2( ), Daniel Thangadurai Thangaian3, Palaniswamy Vijai Bharathy4
Department of Research and Development, Bharathiar University, Tamil Nadu, India
Department of Physics, Hindusthan College of Engineering and Technology, Coimbatore, Tamil Nadu, India
Department of Nanoscience and Technology, Sri Ramakrishna Engineering College, Coimbatore, Tamil Nadu, India
Department of Physics, C.B.M College, Coimbatore, Tamil Nadu, India

Abstract

The present study reports the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Clitoria ternatea flower extract and examination of their antibacterial activity. The change in color of the reaction mixture from violet to yellow colour indicated the formation of ZnO NPs. The formation of ZnO NPs was confirmed by the appearance of a maximum absorption peak at 381 nm in the UV-visible spectrum. The XRD pattern corresponding with the JCPDS card for ZnO showed the presence of purecrystalline ZnO NPs. FTIR spectra confirmed the stretching vibrations of C=O, C–O–H, and O–H groups involved in the reduction of ZnO NPs. The size and morphology of the ZnO NPs were confirmed by Transmission Electron Microscopy (TEM). Further particle size was determined by using Dynamic Light Scattering (DLS). Furthermore, the antibacterial activity of ZnO NPs showed significant inhibitory activity against Escherichia coli and Staphylococcus aureus bacteria.

Keywords: Characterization, XRD, Antibacterial activity, Biosynthesis, Zinc oxide nanoparticles

References(31)

[1]

N.H. A Nguyen, V.V. T Padil, V.I. Slaveykova, et al., Green Synthesis of Metal and Metal Oxide Nanoparticles and Their Effect on the Unicellular Alga Chlamydomonas reinhardtii. Nanoscale Res. Lett, 2018, 13: 159.
DOI Google Scholar
[2]

S. Stankic, S. Suman, F. Haque, J. Vidic, Pure and multi metal oxide nanoparticles: synthesis, antibacterial and cytotoxic properties. J Nanobiotechnol, 2016, 14: 73.
DOI Google Scholar
[3]

N. Tripathy, D. Kim, Metal oxide modified ZnO nanomaterials for biosensor applications. Nano Converg, 2018, 5: 27.
DOI Google Scholar
[4]

A. Czyżowska, A. Barbasz, A review: zinc oxide nanoparticles-friends or enemies? Int. J. Environ. Health Res, 2020, 10: 1-17.
DOI Google Scholar
[5]

D. M Cruz, E. Mostafavi, A. Vernet-Crua, et al., Green nanotechnology-based zinc oxide (ZnO) nanomaterials for biomedical applications: a review. J. Phys. Mater, 2020, 3: 034005.
DOI Google Scholar
[6]

H. Agarwal, S. Kumar, S. Rajeshkumar, A review on green synthesis of zinc oxide nanoparticles – An eco-friendly approach. Resource-Efficient Technologies, 2017, 3: 406-413.
DOI Google Scholar
[7]

R. E Adam, G. Pozina, M. Willander, O. Nur, Synthesis of ZnO nanoparticles by co-precipitation method for solar driven photodegradation of Congo red dye at different pH. Photonics and Nanostructures - Fundam. Appl, 2018, 32: 11-18.
DOI Google Scholar
[8]

D. Bharti, A. V Bharati, Synthesis of ZnO nanoparticles using a hydrothermal method and a study its optical activity. Luminescence, 2016, 32 (3): 317-320.
DOI Google Scholar
[9]

M.F. Khan, A. H Ansari, M. Hameedullah, et al., Sol-gel synthesis of thorn-like ZnO nanoparticles endorsing mechanical stirring effect and their antimicrobial activities: Potential role as nano-antibiotics. Sci Rep, 2016, 6: 27689.
DOI Google Scholar
[10]

A. Jose, K.R.S. Devi, D. Pinheiro, et al., Electrochemical synthesis, photodegradation and antibacterial properties of PEG capped zinc oxide nanoparticles. J. Photochem. Photobiol. B, 2018, 187: 25-34.
DOI Google Scholar
[11]

T. Ghoshal, S. Biswas, M. Paul, et al., Synthesis of ZnO Nanoparticles by Solvothermal Method and Their Ammonia Sensing Properties. J. Nanosci. Nanotechnol, 2009, 9: 5973-5980.
DOI Google Scholar
[12]

M. Hasanpoor, M. Aliofkhazraei, H. Delavari, Microwave-assisted synthesis of zinc oxide nanoparticles. Procedia Material Science, 2015, 11: 320-325.
DOI Google Scholar
[13]

K. Ali, S. Dwivedi, A. Azam, et al., Aloe vera extract functionalized zinc oxide nanoparticles as nanoantibiotics against multi-drug resistant clinical bacterial isolates. J. Colloid Interface Sci, 2016, 472: 145-156.
DOI Google Scholar
[14]

K. Elumalai, S. Velmurugan, S. Ravi, et al., Green synthesis of Zinc oxide nanoparticles using Moringa oleifera leaf extract and evaluation of its antimicrobial activity. Spectrochim. Acta A, 2015, 143: 158-164.
DOI Google Scholar
[15]

N. Senthilkumar, E. NandhaKumar, P. Priya, et al., Synthesis, Anti-bacterial, Anti-arthritic, Anti-oxidant and In-vitro Cytotoxicity Activities of ZnO Nanoparticles Using Leaf Extract of Tectona Grandis (L. ). New J. Chem, 2017, 41: 10347-10356.
DOI Google Scholar
[16]

G. Sathishkumar, Rajkuberan, K. Manikandan, et al., Facile biosynthesis of antimicrobial zinc oxide (ZnO) nanoflakes using leaf extract of Couroupita guianensis, Aubl. Mater. Lett, 2017, 188: 383-386.
DOI Google Scholar
[17]

N. Bala, S. Saha, M. Chakraborty, et al., Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: Effect of temperature on synthesis, anti-bacterial activity and antidiabetic activity. RSC Adv, 2015, 5: 4993-5003.
DOI Google Scholar
[18]

M. Naseer, U. Aslam, B. Khalid, et al., Green route to synthesize Zinc oxide nanoparticles using leaf extracts of Cassia fistula and Melia azadarach and their antibacterial potential. Sci Rep, 2020, 10: 9055.
DOI Google Scholar
[19]

D. Suresh, R. M Shobharani, P.C. Nethravathi, et al., Artocarpus gomezianus aided green synthesis of ZnO nanoparticles: Luminescence, Photocatalytic and Antioxidant Properties. Spectrochim. Acta A, 2015, 141: 128-134.
DOI Google Scholar
[20]

K. Elumalai, S. Velmurugan, S. Ravi, et al., Bio-approach: Plant mediated synthesis of ZnO nanoparticles and their catalytic reduction of methylene blue and antimicrobial activity. Adv Powder Tech, 2015, 26 (6): 1639-1651.
DOI Google Scholar
[21]

N. Jamil, F. Paee, Antimicrobial Activity from Leaf, Flower, Stem, and Root of Clitoria ternatea – A Review. AIP Conf. Proc, 2018, 2002: 020044.
DOI Google Scholar
[22]

N.A. Ludin, M.A.M. Al-Alwani, A. BMohamad, et al., Utilization of Natural Dyes from Zingiber officinale Leaves and Clitoria ternatea Flowers to Prepare New Photosensitisers for Dye-Sensitised Solar Cells. Int. J. Electrochem. Sci, 2018, 13: 7451-7465.
DOI Google Scholar
[23]

K. Rambabu, G. Bharath, F. Banat, et al., Green synthesis of zinc oxide nanoparticles using Phoenix dactylifera waste as bioreductant for effective dye degradation and antibacterial performance in wastewater treatment. J. Hazard. Mater, 2021, 402: 123560.
DOI Google Scholar
[24]

T. Safawo, B. Sandeep, S. Pola, et al., Synthesis and characterization of zinc oxide nanoparticles using tuber extract of anchote (Coccinia abyssinica (Lam. ) Cong. ) for antimicrobial and antioxidant activity assessment. OpenNano, 2018, 3: 56-63.
DOI Google Scholar
[25]

M. Khan, P. Ware, N. Shimpi, Synthesis of ZnO nanoparticles using peels of Passiflora foetida and study of its activity as an efficient catalyst for the degradation of hazardous organic dye. SN Applied Sciences, 2020, 3: 528.
DOI Google Scholar
[26]

M. D McCluskey, A.D. Lalonde, M.G. Norton, et al., Infrared spectroscopy of ZnO nanoparticles containing CO2 impurities. Appl. Phy. Lett. 2005, 86: 073111.
DOI Google Scholar
[27]

S.O. Ogunyemi, Y. Abdallah, M. Zhang, et al., Green synthesis of zinc oxide nanoparticles using different plant extracts and their antibacterial activity against Xanthomonas oryzae pv. oryzae. Artif Cells Nanomed Biotechnol, 2019, 47 (1): 341-352.
DOI Google Scholar
[28]

D. Sharma, R. Jha, Analysis of structural, optical and magnetic properties of Fe/Co co-doped ZnO nanocrystals. Ceram. Int. 2017, 43: 8488-8496.
DOI Google Scholar
[29]

D. Cao, S. Gong, X. Shu, et al., Preparation of ZnO Nanoparticles with High Dispersibility Based on Oriented Attachment (OA) Process. Nanoscale Res. Lett, 2019, 14: 210.
DOI Google Scholar
[30]

M. D Jayappa, C.K. Ramaiah, M.A.P. Kumar, et al., Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro grown callus of Mussaenda frondosa L. : characterization and their applications. Appl. Nanosci, 2020, 10: 3057-3074.
DOI Google Scholar
[31]

Y. N Slavin, J. Asnis, U. O Hafeli, et al., Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J. Nanobiotechnol, 2017, 15: 65.
DOI Google Scholar
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 07 July 2021
Accepted: 04 November 2021
Published: 10 December 2021
Issue date: December 2021

Copyright

© Selvaraj Prabhu, Daniel Thangadurai Thangaian, and Palaniswamy Vijai Bharathy.

Acknowledgements

Acknowledgements

Author SP sincerely acknowledges the Department of Nanoscience and Technology, Sri Ramakrishna Engineering College-Coimbatore, for providing UV, FTIR, XRD analysis and DST-FIST lab facilities, and Hindusthan College of Engineering and Technology, Coimbatore, for providing technical support.

Rights and permissions

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Return