In-vitro Study on Genotoxicity of Green Synthesized Silver Nanoparticles

Marcellin Mecwan; Manita Das; Sonal Thakore; Sonal Rajiv Bakshi

doi:10.5101/nbe.v13i1.p72-81

Nano Biomedicine and Engineering 2021, 13(1): 72-81 https://doi.org/10.5101/nbe.v13i1.p72-81

Research Article |

Open Access | Issue | Published: 19 February 2021

In-vitro Study on Genotoxicity of Green Synthesized Silver Nanoparticles

Show Author's Information Hide Author's Information Marcellin Mecwan^¹, Manita Das^², Sonal Thakore^², Sonal Rajiv Bakshi^¹(

)

Institute of Science, Nirma University, Ahmedabad, Gujarat 382481, India

Department of Chemistry, The Maharaja Sayajirao University of Baroda, Vadodara, India

Keywords:

Characterization, Green synthesis, Ag NPs, DNA binding, Genotoxicity, Chromosomal aberrations

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Mecwan M, Das M, Thakore S, et al. In-vitro Study on Genotoxicity of Green Synthesized Silver Nanoparticles. Nano Biomedicine and Engineering, 2021, 13(1): 72-81. https://doi.org/10.5101/nbe.v13i1.p72-81

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Abstract

The wide use of metal nanoparticles has raised the concern of their release in the environment and their biological safety. The use of silver nanoparticles (Ag NPs) as effective antimicrobial agent is also questioned due to the resistance developed by pathogens and the biosafety for the host. The genotoxicity caused by such particles need to be assessed to ensure that they are safe to use. In this study, green synthesized Ag NPs were used and the biosafety in terms of genotoxicity was evaluated by in vitro Chromosome aberration (CA) assay using short-term cultures of human peripheral blood. The physicochemical parameters of these Ag NPs were studied for stability in nano form. The transmission electron microscopy (TEM) and energy-dispersive spectroscopy (EDS) were carried out. The dynamic light scattering and zeta potential measurements revealed stable nano particles. The UV visible & fluorescence spectroscopy for binding affinity of Ag NPs did not suggest significant DNA interaction. The exposure of whole blood cultures to Ag NPs for 24 h showed dose dependent increase in frequency of CA per cell at 350, 650 and 1000 μL (P = NS, P < 0.05 and P < 0.001 respectively). Our study demonstrates that genotoxicity of Ag NPs can be reduced by the use of green synthesized Ag NPs at low dose exposure, which will guide selecting right concentrations of NP for further in vivo studies, and future applications.

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In-vitro Study on Genotoxicity of Green Synthesized Silver Nanoparticles

Show Author's information Hide Author's Information Marcellin Mecwan^¹, Manita Das^², Sonal Thakore^², Sonal Rajiv Bakshi^¹(

)

Institute of Science, Nirma University, Ahmedabad, Gujarat 382481, India

Department of Chemistry, The Maharaja Sayajirao University of Baroda, Vadodara, India

Abstract

Keywords: Characterization, Green synthesis, Ag NPs, DNA binding, Genotoxicity, Chromosomal aberrations

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Acknowledgements

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Publication history

Received: 13 August 2020

Accepted: 18 January 2021

Published: 19 February 2021

Issue date: March 2021

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Acknowledgements

Authors are grateful to Nirma University for the financial support.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.