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Nano Biomedicine and Engineering 2021, 13(1): 20-26 https://doi.org/10.5101/nbe.v13i1.p20-26
Research Article | Open Access | Issue | Published: 21 November 2020

Histological, Biochemical and DNA Changes in the Liver of Male Albino Rats Treated with Silver Nanoparticles

Show Author's Information Abed Hassan Baraaj( )
Department of Biology, Collage of Sciences-University of Baghdad, Baghdad, Iraq
Keywords:
Silver, Nanoparticles, DNA, Enzymes, Liver, Histopathological, Comet assay
Cite this article:
Baraaj AH. Histological, Biochemical and DNA Changes in the Liver of Male Albino Rats Treated with Silver Nanoparticles. Nano Biomedicine and Engineering, 2021, 13(1): 20-26. https://doi.org/10.5101/nbe.v13i1.p20-26
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Abstract Full text About this article

Sliver nanoparticles (Ag NPs) are produced industrially and commercially available in recent time. Silver and its nanoparticles are widely applied to consuming medical purposes. Silver nanoparticles are able to resemble cellular components and typical proteins, which helps the nanoparticles to cross natural barriers in human body. For this reason, the current study was designed to investigate the damage caused by silver nanoparticles, especially in liver which is one of the most targeted organs of nanoparticles as a result of their accumulation and damages caused in various aspects such as tissue, functional ability and DNA of liver cells. 30 albino male rats were used for this study and were divided into 3 main groups. The first group represented control and a normal saline solution was given, while the second group was given silver nanoparticles at a concentration of 2 mg/kg of body weight, and the third group was given silver nanoparticles at a concentration of 3 mg/kg of body weight. Each concentration of Ag NPs was dissolved in 3 mL of the normal saline and given directly to the experimental animal's stomach by feeding tube with diameter of 0.6 cm and every day for 10 weeks. The results in the current study showed histopathological change in liver tissue such as large granulomatous lesion with an increase in macrophage numbers, as also shown by necrotic hepatocytes with dilation of sinusoids. The deformation of architecture in the parenchyma appeared in both concentrations. The biochemical parameters investigation in the liver clarified increased level of enzymes accordance with doses of Ag NPs. The damage was identified in DNA of hepatocytes by comet assay and the degree of damage was evident through increasing the numbers of tailed nuclei and in strand breaks of DNA. The reactive oxygen species (ROS) generation had a key role in the events of structural and functional disorders in the liver of experimental animals.


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About this article

Histological, Biochemical and DNA Changes in the Liver of Male Albino Rats Treated with Silver Nanoparticles

Show Author's information Abed Hassan Baraaj( )
Department of Biology, Collage of Sciences-University of Baghdad, Baghdad, Iraq

Abstract

Sliver nanoparticles (Ag NPs) are produced industrially and commercially available in recent time. Silver and its nanoparticles are widely applied to consuming medical purposes. Silver nanoparticles are able to resemble cellular components and typical proteins, which helps the nanoparticles to cross natural barriers in human body. For this reason, the current study was designed to investigate the damage caused by silver nanoparticles, especially in liver which is one of the most targeted organs of nanoparticles as a result of their accumulation and damages caused in various aspects such as tissue, functional ability and DNA of liver cells. 30 albino male rats were used for this study and were divided into 3 main groups. The first group represented control and a normal saline solution was given, while the second group was given silver nanoparticles at a concentration of 2 mg/kg of body weight, and the third group was given silver nanoparticles at a concentration of 3 mg/kg of body weight. Each concentration of Ag NPs was dissolved in 3 mL of the normal saline and given directly to the experimental animal's stomach by feeding tube with diameter of 0.6 cm and every day for 10 weeks. The results in the current study showed histopathological change in liver tissue such as large granulomatous lesion with an increase in macrophage numbers, as also shown by necrotic hepatocytes with dilation of sinusoids. The deformation of architecture in the parenchyma appeared in both concentrations. The biochemical parameters investigation in the liver clarified increased level of enzymes accordance with doses of Ag NPs. The damage was identified in DNA of hepatocytes by comet assay and the degree of damage was evident through increasing the numbers of tailed nuclei and in strand breaks of DNA. The reactive oxygen species (ROS) generation had a key role in the events of structural and functional disorders in the liver of experimental animals.

Keywords: Silver, Nanoparticles, DNA, Enzymes, Liver, Histopathological, Comet assay

References(33)

[1]

J.F. Hillyer, R.M. Albrecht, Gastrointestinal per sorption and tissue distribution of differently sized colloidal gold nanoparticles. J. Pharm. Sci., 2001, 90: 1927-1936.
DOI Google Scholar
[2]

K. Dziendzi kowska, J. Gromadzka-Ostowska, A. Lankoff, et al., Time dependent biodistribution and excretion of silver nanoparticles in male wester rats. J. Appl. Toxicol., 2010, 32: 920-928.
DOI Google Scholar
[3]

M. Walker, D. Parsons, The biological fate of silver ions following the use of silver-containing wound care products - a review. Int. Wound J., 2012.
DOI Google Scholar
[4]

Z. Wang, N. Li, J. Zhao, et al., CuO nanoparticle interaction with human epithelial cells: cellular uptake location, Export and genotoxicity. Chem. Res. Toxicol., 2012, 25: 1512-1521.
DOI Google Scholar
[5]

R. De Lima, A.B. Eabra, and N. Duran, Silver nanoparticles: brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles. J. Appl. Toxicol., 2012, 32: 867-869.
DOI Google Scholar
[6]

V. Rabolli, L.C. Thomassen, F. Uwambayinema, et al., The cytotoxic activity of amorphous silica nanoparticles is mainly influenced by surface area and not by aggregation. Toxicol. Lett., 2011, 206: 197-203.
DOI Google Scholar
[7]

S. Fraga, H. Faria, M.E. Soares, et al., Influence of the surface coating on the cytotoxicity, genotoxicity and uptake of gold nanoparticles in human HepG2 cells. J. Appl. Toxical., 2013, 33: 1111-1119.
DOI Google Scholar
[8]

V. Edwards-Jones, The benefits of silver in hygiene, personal care and health care. Lett. Appl. Microbiol., 2009, 49(2): 147-152.
DOI Google Scholar
[9]

S.W. Hoven, W.J. Peijnenburg, C.A. Herberts, et al., Nano-silver a review of available data and know Ledge gaps in human and Environmental risk assessment. Nanotoxicology, 2009, 3(2): 109-138.
DOI Google Scholar
[10]

C. Brandenberger, C. Muhlfeld, Z. Ali, et al., Quantitative evaluation of cellular up-take and trafficking of plain and polyethylene glycol-coated gold nanoparticles. Small, 2010, 6: 1669-1678.
DOI Google Scholar
[11]

J. Skalska, L. Struzynska, Z.D. Makarewie, et al., Toxic effects of silver nanoparticles in mammals dose a risk of neurotoxicity exist. Folianeuropathol., 2015, 53(4): 281-300.
DOI Google Scholar
[12]

A.H. Baraaj, Histological and physiological effect of Chitocal on liver, some parameters of blood and body weight in male Albino Rats. Magazine of Al-Kufa University for Biology, 2014, 6(1): 1-8.
Google Scholar
[13]
S.K. Suvarna, C. Layton. and J.D. Bancr, Histological preperationsBancroft theory and practice of histological techniques, 7th edition. Elsevier, 2013: 233-250.
[14]

M. De Boeck, N. Touil, G. De Visscher, et al., Validation and Implementation of an internal standard in comet assay. Mutat. Res., 2000, 469: 181-197.
DOI Google Scholar
[15]
B. Rosner, Fundamentals of biostatistics. Thomson Brooks Cole, Cengage Learning, 2010: 150-151.
[16]

H.H. Gustafson, D. Holt-Casper, D.W. Grainger, et al., Nanoparticles uptake: The phagocyte problem. Nano. Today, 2015, 10(4): 487-510.
DOI Google Scholar
[17]

H.I. Herd, K.T. Bartlett, and J.A. Gustafson, Macrophage silica nanoparticle response is phenotypically dependent. Biomaterials, 2015, 53: 574-582.
DOI Google Scholar
[18]
D.A. Kuhn, D. Vanhecke, B. Michen, et al., Different endocytic uptake mechanisms for nanoparticles in epithelial cells and macrophages. Beilstein J. Nanotechnol, 2014, 5: 1625-1636. S. Shrivastava, T. Bera, G. Singh, et al., Characterization of antiplatelet properties of silver nanoparticles. ACS Nano, 2009, 3(6): 1357-1364.
DOI
[19]

J.T. Kwon, A.H. Minai-Tehrani, S.K. Wang, et al., Acute pulmonary toxicity and body distribution of inhaled metallic silver nanoparticles. Toxicological Research, 2012, 28(1): 25-31.
DOI Google Scholar
[20]

A.L. Monfared, S. Soltani, Effect of silver nanoparticles administration on the liver of rainbow trout (Oncorhynchus mykiss): histological and biochemical studies. European Journal of Experimental Biology, 2013, 3(2): 285-289.
Google Scholar
[21]

N. Elyamany, H.A. Kholoussi, D.F. Raouf, et al., Nigella sativa and Aloe vera protection against doxorubicin in hepatotoxicity. J. Exp. Appl. Anim. Sci. , 2016, 2(1): 10-22.
DOI Google Scholar
[22]

A. Singh, T.K. Bhat, and O.P. Sharma, Clinical Biochemistry of Hepatotoxicity. J. Clin. Toxicol. , 2011, 4: 1-19.
Google Scholar
[23]

E.J. Park, E. Bae, J. Yi, et al., Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environmental Toxicology and Pharmacology, 2010, 30(2): 162-168.
DOI Google Scholar
[24]

J.H. Lee, Y.S. Kim, K.S. Song, et al., Bio persistence of silver nanoparticles in tissues from Sprague-Dawley rats. Particle and Fiber Toxicology, 2013, 10(1): 1.
DOI Google Scholar
[25]

P.V. Asharani, M.P. Hande, and L.S. Valiyaveetti, Anti-proliferative activity of silver nanoparticles. BMC Cell Biology, 2009, 10(1): 1.
DOI Google Scholar
[26]

S. Hackenberg, A. Scherzed, M. Kessler, et al., Silver nanoparticles evaluation of DNA damage, toxicity and functional impairment in human mesenchymal stem cells. Toxicology Letters, 2011, 201(1): 27-33.
DOI Google Scholar
[27]

R. Foldbjerg, P. Olesen, M. Hougaard, et al., PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, Apoptosis and necrosis in THP-1 monocytes. Toxicology Letters, 2009, 192(2): 156-162.
DOI Google Scholar
[28]

R.P. Singh, P. Ramarao, Cellular uptake, intracellular trafficking and cytotoxicity of silver nanoparticles. Toxicology Letters, 2012, 213(2): 249-259.
DOI Google Scholar
[29]

Y. Xue, S. Zhang, Y. Huang, et al., Acute toxic effects and gender-related biokinetics of silver nanoparticles following an intravenous injection in mice. Journal of Applied Toxicology, 2012, 32(11): 890-899.
DOI Google Scholar
[30]

R. Hess, L. Jones, and J.J. Schlager, Unique cellular Interaction of silver nanoparticles: size-dependent generation of reaction oxygen species. J. Phys. Chem. B, 2008, 112: 13608-13619.
DOI Google Scholar
[31]

Y.H. Hsin, C.F. Chen, S. Huang, et al., The apoptotic effect of nano silver is mediated by a ROS-and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T4 Cells. Toxicology Letters, 2008, 179(3): 130-139.
DOI Google Scholar
[32]

W. Liu, Y. Wu, C. Wang, et al., Impact of silver nanoparticles on human cells: effect of particle size. Nanotoxicology B, 2010, 4(3): 319-330.
DOI Google Scholar
[33]

S.H. Kim, J.W. Ko, S.K. Koh, et al., Silver nanoparticles induce apoptotic cell death in cultured cerebral cortical neurons. Molecular & cellular Toxicology, 2014, 10(2): 173-179.
DOI Google Scholar
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Publication history

Received: 19 May 2020
Accepted: 28 August 2020
Published: 21 November 2020
Issue date: March 2021

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© Abed Hassan. Baraaj.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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