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Research Article | Open Access

Development of SARS-CoV-2 Isothermal Amplification Detection Kits

Xueling Li1, Jing Tian1, Yan Xu1, Xiaomin Chen1,2, Zexi Liu1, Hui Liang1, Caihong Jin1, Daxiang Cui1,2( )
National Engineering Research Center for Nanotechnology, Shanghai, China
Department of Instrument Science and Engineering, Shanghai Jiao Tong University, Shanghai, China
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Abstract

The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents. After the isothermal amplification reactions were completed, the reaction results were judged by using the chromogenic agents to determine whether SARS-CoV-2 exists in the samples to be tested. The detection kits have the advantages of convenient operation, fast detection speed and high sensitivity up to 1 copy of virus particles per reaction, which can speed up the detection speed of suspected cases, and avoid the missing detection problems caused by the low detection sensitivity.

Keywords

COVID-19SARS-CoV-2Detection kitsIsothermal amplificationLoop-mediated isothermal amplification

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Nano Biomedicine and Engineering
Volume 12 Issue 4,
December 2020
Pages 316-320
DOI: 10.5101/nbe.v12i4.p316-320

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Cite this article:
Li X, Tian J, Xu Y, et al. Development of SARS-CoV-2 Isothermal Amplification Detection Kits. Nano Biomedicine and Engineering, 2020, 12(4): 316-320. https://doi.org/10.5101/nbe.v12i4.p316-320

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Received: 24 August 2020
Accepted: 28 October 2020
Published: 28 October 2020
© Xueling Li, Jing Tian, Yan Xu, Xiaomin Chen, Zexi Liu, Hui Liang, Caihong Jin, and Daxiang Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.