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Nano Biomedicine and Engineering 2020, 12(3): 272-280 https://doi.org/10.5101/nbe.v12i3.p272-280
Research Article | Open Access | Issue | Published: 23 September 2020

Biosynthesis of Silver Nanoparticles from Streptomyces Spp., Characterization and Evaluating of Its Efficacy against Phomopsis theae and Poria hypolateria in Tea Plants (Camellia sinensis)

Show Author's Information Karthik Natesan1,  Ponnusamy Ponmurugan1( ),  Balasubramanian Mythili Gnanamangai2,  Mani Suganya2,  Shivasji Kavitha2
Department of Botany, Bharathiar University, Coimbatore - 641 046, Tamil Nadu, India
Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode - 637 215, Tamil Nadu, India
Keywords:
Silver nanoparticles, Streptomyces, Biosynthesis of nanoparticles, Phomopsis, Poria
Cite this article:
Natesan K, Ponmurugan P, Gnanamangai BM, et al. Biosynthesis of Silver Nanoparticles from Streptomyces Spp., Characterization and Evaluating of Its Efficacy against Phomopsis theae and Poria hypolateria in Tea Plants (Camellia sinensis). Nano Biomedicine and Engineering, 2020, 12(3): 272-280. https://doi.org/10.5101/nbe.v12i3.p272-280
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Abstract Full text About this article

The biosynthesis of metal nanoparticles is an emerging area of advanced research in plant protection. In this study, an eco-friendly and rapid protocol was developed where silver nanoparticles were biosynthesized by using Streptomyces spp. The biocontrol agent was isolated from different tea soils and the efficient strain (VASC201) was identified by dual culture and antibiosis. Nanoparticles were successfully synthesized from this efficient strain. The silver nanoparticles exhibited their resonance peak at 448 nm under ultraviolet-visible spectroscopy (UV-Vis). The structural analysis of the silver nanoparticles synthesized by the Streptomyces spp. exhibited a strong crystalline structure. The morphology and chemical composition of the silver nanoparticle were examined by high-resolution transmission electron microscopy (HR-TEM) equipped with energy-dispersive X-ray spectrometer (EDX) and were predominantly spherical in shape and uniformly distributed without significant agglomeration. The average crystalline size of the prepared silver nanoparticle was found to be 51.2 nm. The stretching vibrational peak at 3420, 2920 and 2350 cm-1 in Fourier-transform infrared spectroscopy (FTIR) were attributed to various functional group of metal nanoparticle synthesis. We assessed the effectiveness of silver nanoparticles against Poria hypolateritia and Phomopsis theae. The results showed that the nanoparticles showed very good inhibitory effect of about 69.90% inhibition of Poria at 5 ppm concentration on the 15th day. Phomopsis was inhibited to 67.47% at 5 ppm of silver nanoparticles at the same time interval.


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Biosynthesis of Silver Nanoparticles from Streptomyces Spp., Characterization and Evaluating of Its Efficacy against Phomopsis theae and Poria hypolateria in Tea Plants (Camellia sinensis)

Show Author's information Karthik Natesan1, Ponnusamy Ponmurugan1( ), Balasubramanian Mythili Gnanamangai2, Mani Suganya2, Shivasji Kavitha2
Department of Botany, Bharathiar University, Coimbatore - 641 046, Tamil Nadu, India
Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode - 637 215, Tamil Nadu, India

Abstract

The biosynthesis of metal nanoparticles is an emerging area of advanced research in plant protection. In this study, an eco-friendly and rapid protocol was developed where silver nanoparticles were biosynthesized by using Streptomyces spp. The biocontrol agent was isolated from different tea soils and the efficient strain (VASC201) was identified by dual culture and antibiosis. Nanoparticles were successfully synthesized from this efficient strain. The silver nanoparticles exhibited their resonance peak at 448 nm under ultraviolet-visible spectroscopy (UV-Vis). The structural analysis of the silver nanoparticles synthesized by the Streptomyces spp. exhibited a strong crystalline structure. The morphology and chemical composition of the silver nanoparticle were examined by high-resolution transmission electron microscopy (HR-TEM) equipped with energy-dispersive X-ray spectrometer (EDX) and were predominantly spherical in shape and uniformly distributed without significant agglomeration. The average crystalline size of the prepared silver nanoparticle was found to be 51.2 nm. The stretching vibrational peak at 3420, 2920 and 2350 cm-1 in Fourier-transform infrared spectroscopy (FTIR) were attributed to various functional group of metal nanoparticle synthesis. We assessed the effectiveness of silver nanoparticles against Poria hypolateritia and Phomopsis theae. The results showed that the nanoparticles showed very good inhibitory effect of about 69.90% inhibition of Poria at 5 ppm concentration on the 15th day. Phomopsis was inhibited to 67.47% at 5 ppm of silver nanoparticles at the same time interval.

Keywords: Silver nanoparticles, Streptomyces, Biosynthesis of nanoparticles, Phomopsis, Poria

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Publication history

Received: 17 February 2020
Accepted: 14 July 2020
Published: 23 September 2020
Issue date: September 2020

Copyright

© Karthik Natesan, Ponnusamy Ponmurugan, Balasubramanian Mythili Gnanamangai, Mani Suganya, and Shivasji Kavitha.

Acknowledgements

Acknowledgements

The authors are thankful to the National Tea Research Foundation (NTRF), Kolkata, for financial support.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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