Agro-Industrial Waste-Mediated Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity

The development of immaculate etiquette for the green and rapid synthesis of Ag NPs with a natural reducing agent is the spearhead of the expanding field of nanotechnology. Different scientific fraternity with novel natural reducing agents has been contributing numerous strategies daily. Though there is a submerging of many natural reducing agents, still there are plenty of natural precursors remained to be explored. In this research, we fruitfully attempted the synthesis of silver nanoparticles using agro-food industrial waste Tamarindus indica shell-husk extract (TSE) as a natural reducing agent. The prepared silver nanoparticles and their stability in different pH were investigated using ultraviolet-visible spectroscopic analysis. Morphological characters were examined using scanning electron microscope (SEM) and transmission electron microscopy (TEM) analysis. The structural and elemental compositions were depicted by Fourier-transform infrared spectroscopy (FTIR) and energy-dispersive X-ray (EDX) analysis, respectively. Moreover, we emphasized on the molecular mechanism involving in the TSE mediated synthesis of Ag NPs. The inherent antimicrobial activity was investigated using agar plate method against both gram-positive and gram-negative species with gentamycin as a control standard for comparison.