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Nano Biomedicine and Engineering 2019, 11(4): 381-390 https://doi.org/10.5101/nbe.v11i4.p381-390
Research Article | Open Access | Issue | Published: 13 December 2019

Application of Silver Nanoparticles for the Spectrophotometric Determination of Cefdinir and Cefepime HCl in Pharmaceutical Preparations and Human Urine Samples

Show Author's Information Walaa El-Alfy( ),  Omnia Ahmed Ismaiel,  Magda Youssef El-Mammli,  Abdalla Shalaby
Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
Keywords:
Silver nanoparticles, Cefdinir, Cefepime HCl, Human urine samples
Cite this article:
El-Alfy W, Ismaiel OA, El-Mammli MY, et al. Application of Silver Nanoparticles for the Spectrophotometric Determination of Cefdinir and Cefepime HCl in Pharmaceutical Preparations and Human Urine Samples. Nano Biomedicine and Engineering, 2019, 11(4): 381-390. https://doi.org/10.5101/nbe.v11i4.p381-390
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Abstract Full text About this article

In this work, the application of silver nanoparticles (Ag NPs) for spectrophotometric determination of cefdinir (CFD) and cefepime HCl (CFP) was developed. The method was based on the ability of the alkaline degradation products of the cited drugs to reduce Ag+ ions to silver nanoparticles (Ag-NPs) in the presence of gelatin as a capping agent. The linear concentration ranges were 5.0-40 and 10-100 μg/mL for cefdinir and cefepime HCl, respectively. The lower detection limits were found to be 0.39 and 1.06 μg/mL for CFD and CFP, respectively, while the quantitation limits were found to be 1.18 and 3.21 μg/mL for CFD and CFP, respectively. The formed nanoparticles were characterized by ultraviolet-visible spectroscopy and transmission electron microscopy. The proposed method was applied successfully to determination of CFD and CFP in pure drugs, pharmaceutical preparations and human urine samples.


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About this article

Application of Silver Nanoparticles for the Spectrophotometric Determination of Cefdinir and Cefepime HCl in Pharmaceutical Preparations and Human Urine Samples

Show Author's information Walaa El-Alfy( ), Omnia Ahmed Ismaiel, Magda Youssef El-Mammli, Abdalla Shalaby
Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt

Abstract

In this work, the application of silver nanoparticles (Ag NPs) for spectrophotometric determination of cefdinir (CFD) and cefepime HCl (CFP) was developed. The method was based on the ability of the alkaline degradation products of the cited drugs to reduce Ag+ ions to silver nanoparticles (Ag-NPs) in the presence of gelatin as a capping agent. The linear concentration ranges were 5.0-40 and 10-100 μg/mL for cefdinir and cefepime HCl, respectively. The lower detection limits were found to be 0.39 and 1.06 μg/mL for CFD and CFP, respectively, while the quantitation limits were found to be 1.18 and 3.21 μg/mL for CFD and CFP, respectively. The formed nanoparticles were characterized by ultraviolet-visible spectroscopy and transmission electron microscopy. The proposed method was applied successfully to determination of CFD and CFP in pure drugs, pharmaceutical preparations and human urine samples.

Keywords: Silver nanoparticles, Cefdinir, Cefepime HCl, Human urine samples

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Publication history
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Publication history

Received: 19 July 2019
Accepted: 27 November 2019
Published: 13 December 2019
Issue date: December 2019

Copyright

© GhWalaa El-Alfy, Omnia Ahmed Ismaiel, Magda Youssef El-Mammli, and Abdalla Shalaby.

Acknowledgements

This work was supported by Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Egypt. The authors wish to express their gratitude for the support of this work.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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