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Nano Biomedicine and Engineering 2019, 11(2): 200-214 https://doi.org/10.5101/nbe.v11i2.p200-214
Research Article | Open Access | Issue | Published: 20 June 2019

Boosting Antimicrobial Activity of Imipenem in Combination with Silver Nanoparticles towards S. fonticola and Pantoea sp.

Show Author's Information Shaimaa Obaid Hasson1( ),  Mohammed Jabber Al-Awady2,  Adnan Hamad Al-Hamadani3,  Ibtisam Habeeb Al-Azawi3,  Alaa Irhayyim Ali3
Department of Microbiology, College of Veterinary, Al-Qasim Green University, Iraq
Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq
Department of Medical Microbiology, College of Medicine, Al-Qadisiyah University, Iraq
Keywords:
Silver nanoparticles, Biofilm, Serratia fonticola, Pantoea sp., Enterobacter cloacae, Imipenem
Cite this article:
Hasson SO, Al-Awady MJ, Al-Hamadani AH, et al. Boosting Antimicrobial Activity of Imipenem in Combination with Silver Nanoparticles towards S. fonticola and Pantoea sp.. Nano Biomedicine and Engineering, 2019, 11(2): 200-214. https://doi.org/10.5101/nbe.v11i2.p200-214
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Abstract Full text Electronic supplementary material About this article

Silver nanoparticles have been considered as powerful antimicrobial agents recently, especially with the increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens. The aim of this study was to synthesize silver nanoparticles by biological and chemical methods and combination with imipenem to eradicate biofilm-forming bacteria at phenotypic and genotypic levels. The biosynthesis of silver nanoparticles was done by using Enterobacter cloacae (cell-free suspension) while chemosynthesis was conducted using sodium borohydride. Biological and chemical silver nanoparticles were characterized by ultraviolet-visible spectrophotometry which showed absorbance peak at 400 and 390nm respectively. Fourier transformer infrared analysis revealed that carboxylic and polyphenolic groups were coated on surface of both silver nanoparticles. Scanning electron microscope and size analyser showed that the sizes of biologically and chemically silver nanoparticles were 63 nm and 25 nm, respectively. In addition, it showed the formation of cubical nanoparticles. The antimicrobial effect of synthesized silver nanoparticles were evaluated by agar well diffusion and macrodilution method to determine minimum inhibitory concentration value. The results showed that biological silver nanoparticles were more effective on biofilm forming bacteria (Serratia fonticola and Pantoea sp.) than chemical synthesized ones. In addition, the combination effect between silver nanoparticles and imipenem displayed synergistic effect. Gene expression of biofilm encoding genes (smaI and esaL) were evaluated by real- time quantitative polymerase chain reaction (RT- qPCR) before and after treatment with silver nanoparticles in both types and imipenem and in combination between them. The results revealed that biological silver nanoparticles alone or in combination with antibiotics were more effective on biofilm gene expression by down regulation than other treatments.


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About this article

Boosting Antimicrobial Activity of Imipenem in Combination with Silver Nanoparticles towards S. fonticola and Pantoea sp.

Show Author's information Shaimaa Obaid Hasson1( ), Mohammed Jabber Al-Awady2, Adnan Hamad Al-Hamadani3, Ibtisam Habeeb Al-Azawi3, Alaa Irhayyim Ali3
Department of Microbiology, College of Veterinary, Al-Qasim Green University, Iraq
Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq
Department of Medical Microbiology, College of Medicine, Al-Qadisiyah University, Iraq

Abstract

Silver nanoparticles have been considered as powerful antimicrobial agents recently, especially with the increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens. The aim of this study was to synthesize silver nanoparticles by biological and chemical methods and combination with imipenem to eradicate biofilm-forming bacteria at phenotypic and genotypic levels. The biosynthesis of silver nanoparticles was done by using Enterobacter cloacae (cell-free suspension) while chemosynthesis was conducted using sodium borohydride. Biological and chemical silver nanoparticles were characterized by ultraviolet-visible spectrophotometry which showed absorbance peak at 400 and 390nm respectively. Fourier transformer infrared analysis revealed that carboxylic and polyphenolic groups were coated on surface of both silver nanoparticles. Scanning electron microscope and size analyser showed that the sizes of biologically and chemically silver nanoparticles were 63 nm and 25 nm, respectively. In addition, it showed the formation of cubical nanoparticles. The antimicrobial effect of synthesized silver nanoparticles were evaluated by agar well diffusion and macrodilution method to determine minimum inhibitory concentration value. The results showed that biological silver nanoparticles were more effective on biofilm forming bacteria (Serratia fonticola and Pantoea sp.) than chemical synthesized ones. In addition, the combination effect between silver nanoparticles and imipenem displayed synergistic effect. Gene expression of biofilm encoding genes (smaI and esaL) were evaluated by real- time quantitative polymerase chain reaction (RT- qPCR) before and after treatment with silver nanoparticles in both types and imipenem and in combination between them. The results revealed that biological silver nanoparticles alone or in combination with antibiotics were more effective on biofilm gene expression by down regulation than other treatments.

Keywords: Silver nanoparticles, Biofilm, Serratia fonticola, Pantoea sp., Enterobacter cloacae, Imipenem

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Received: 28 March 2019
Accepted: 11 June 2019
Published: 20 June 2019
Issue date: June 2019

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© Shaimaa Obaid Hasson, Mohammed Jabber Al-Awady, Adnan Hamad Al-Hamadani, Ibtisam Habeeb Al-Azawi, and Alaa Irhayyim Ali.

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