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Nano Biomedicine and Engineering 2019, 11(2): 157-169 https://doi.org/10.5101/nbe.v11i2.p157-169
Research Article | Open Access | Issue | Published: 10 May 2019

Investigation of Anti-MRSA and Anticancer Activity of Eco-Friendly Synthesized Silver Nanoparticles from Palm Dates Extract

Show Author's Information Mohammed Jabber Al-Awady1( ),  Asim Alaa Balakit2,  Sharafaldin Al-Musawi1,  Mohammed Jassim Alsultani3,  Ahmed Kamil2,  Muntadher Alabbasi2
Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq
Department of Phrmaceutical Chemistry, College of Pharmacy, Babylon University, Babylon, Iraq
Department of Physics, College of Science, Babylon University, Babylon, Iraq
Keywords:
Silver nanoparticles, Green synthesis, Antimicrobial activity, Anticancer activity, Palm dates extract
Cite this article:
Al-Awady MJ, Balakit AA, Al-Musawi S, et al. Investigation of Anti-MRSA and Anticancer Activity of Eco-Friendly Synthesized Silver Nanoparticles from Palm Dates Extract. Nano Biomedicine and Engineering, 2019, 11(2): 157-169. https://doi.org/10.5101/nbe.v11i2.p157-169
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Abstract Full text About this article

In the present work, we investigated novel, green, and facile approach for the synthesis of stable silver nanoparticles by use of inexpensive and available Iraqi Zahdi palm dates extract as reducing and stabilizing agents. The formation and color change of dark brown suspension of silver nanoparticles were monitored and examined by ultraviolet-visible spectrophotometric analysis at maximum wavelength of 401 nm. The produced nanoparticles were characterized by dynamic light scattering (DLS), Fourier transform-infrared spectrometry (FTIR), atomic force microscope (AFM), X-ray diffraction (XRD) and scanning electron microscopy (SEM)-energy dispersive spectroscopy (EDS). The results demonstrated that particle size distribution was around 90 ± 40 nm which was in line with grain size of the nanoparticles measured by AFM, while the crystallite size was about 20 nm according to the results observed from XRD technique. Additionally, the zeta potential of synthesized silver nanoparticles was found to be nearly about -25 mV, whereby the suspension showed high stability upon storing the sample in a cool place for a long period of time. The synthesized silver nanoparticles exhibited efficient antibacterial activity against pathogenic methicillin-resistant Staphylococcus aureus (MRSA) bacteria. They also showed enhanced anticancer activity against breast cancer cells (MCF7) with high apoptotic effect as compared with normal MCF10A cells, which confirmed the biocompatible nature of green synthesized silver nanoparticles. It is considered that nanosilver particles synthesized with palm dates extract may basically establish a significant step to synthesize such nanoparticles for topical applications as well as an alternative anticancer drug of existing chemotherapeutics of cancer..


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About this article

Investigation of Anti-MRSA and Anticancer Activity of Eco-Friendly Synthesized Silver Nanoparticles from Palm Dates Extract

Show Author's information Mohammed Jabber Al-Awady1( ), Asim Alaa Balakit2, Sharafaldin Al-Musawi1, Mohammed Jassim Alsultani3, Ahmed Kamil2, Muntadher Alabbasi2
Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq
Department of Phrmaceutical Chemistry, College of Pharmacy, Babylon University, Babylon, Iraq
Department of Physics, College of Science, Babylon University, Babylon, Iraq

Abstract

In the present work, we investigated novel, green, and facile approach for the synthesis of stable silver nanoparticles by use of inexpensive and available Iraqi Zahdi palm dates extract as reducing and stabilizing agents. The formation and color change of dark brown suspension of silver nanoparticles were monitored and examined by ultraviolet-visible spectrophotometric analysis at maximum wavelength of 401 nm. The produced nanoparticles were characterized by dynamic light scattering (DLS), Fourier transform-infrared spectrometry (FTIR), atomic force microscope (AFM), X-ray diffraction (XRD) and scanning electron microscopy (SEM)-energy dispersive spectroscopy (EDS). The results demonstrated that particle size distribution was around 90 ± 40 nm which was in line with grain size of the nanoparticles measured by AFM, while the crystallite size was about 20 nm according to the results observed from XRD technique. Additionally, the zeta potential of synthesized silver nanoparticles was found to be nearly about -25 mV, whereby the suspension showed high stability upon storing the sample in a cool place for a long period of time. The synthesized silver nanoparticles exhibited efficient antibacterial activity against pathogenic methicillin-resistant Staphylococcus aureus (MRSA) bacteria. They also showed enhanced anticancer activity against breast cancer cells (MCF7) with high apoptotic effect as compared with normal MCF10A cells, which confirmed the biocompatible nature of green synthesized silver nanoparticles. It is considered that nanosilver particles synthesized with palm dates extract may basically establish a significant step to synthesize such nanoparticles for topical applications as well as an alternative anticancer drug of existing chemotherapeutics of cancer..

Keywords: Silver nanoparticles, Green synthesis, Antimicrobial activity, Anticancer activity, Palm dates extract

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Publication history
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Publication history

Received: 11 December 2018
Accepted: 30 April 2019
Published: 10 May 2019
Issue date: June 2019

Copyright

© Mohammed Jabber Al-Awady, Asim Alaa Balakit, Sharafaldin Al-Musawi, Mohammed Jassim Alsultani, Ahmed Kamil, and Muntadher Alabbasi.

Acknowledgements

Acknowledgements

Authors would like to thank SEM Section Team at the University of Babylon, Babylon, Iraq and Dr. Mohammed Sabbar, College of Pharmacy, Basrah University, Basrah, Iraq for their generous contributions in the conduction of this article.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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