Effects of Glucose, Metformin, and Protein on Formation of Flower-like Nanocomposites of Struvite in Infected Artificial Urine Medium by Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA): New Report

Nadia Asadi; Mojtaba Taran; Maryam Rad; Mehran Alavi

doi:10.5101/nbe.v11i1.p91-97

Nano Biomedicine and Engineering 2019, 11(1): 91-97 https://doi.org/10.5101/nbe.v11i1.p91-97

Research Article |

Open Access | Issue | Published: 28 March 2019

Effects of Glucose, Metformin, and Protein on Formation of Flower-like Nanocomposites of Struvite in Infected Artificial Urine Medium by Methicillin-resistant Staphylococcus aureus (MRSA): New Report

Show Author's Information Hide Author's Information Nadia Asadi^¹, Mojtaba Taran^{²^,³}, Maryam Rad^², Mehran Alavi^{²^,³}(

)

Nephrology Department, Kermanshah University of Medicinal Sciences, Kermanshah, Iran

Microbiology Department, Razi University, Kermanshah, Iran

Nanobiotechnology Department, Razi University, Kermanshah, Iran

Keywords:

Staphylococcus aureus, Metformin, Urinary stone disease, Struvite nanocomposites, Diabetic patients, Artificial urine medium

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Asadi N, Taran M, Rad M, et al. Effects of Glucose, Metformin, and Protein on Formation of Flower-like Nanocomposites of Struvite in Infected Artificial Urine Medium by Methicillin-resistant Staphylococcus aureus (MRSA): New Report. Nano Biomedicine and Engineering, 2019, 11(1): 91-97. https://doi.org/10.5101/nbe.v11i1.p91-97

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Abstract

Urinary tract infections (UTIs) are the reason for 15-70% of urinary stone disease (USD). Many diabetic patients suffer from USD resulting from bacterial infections by multi drug resistant (MDR) bacteria such as Staphylococcus aureus ATCC 43300. These bacteria can enhance struvite stones' formation in diabetic patients with UTIs. In this regard, hyperglycemia, proteinuria, and using of metformin drug may be important factors. Three parameters including glucose, metformin, and protein with their three levels of concentrations were determined by experimental design of Taguchi method to obtain optimized formation of struvite stones. Artificial urine medium was utilized for simulation of natural human urine. Scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDAX) techniques were applied for evaluation of morphology and elemental compositions of formed calcium phosphate. This study showed highest concentration of struvite stones (0.512 g/L) under interaction conditions of 3×2×2 levels respectively for glucose, metformin, and protein. Results of SEM and EDAX analyses demonstrated clumped crystallite property and flower-like nanocomposites (NCs) of struvite stones with contribution of calcium (160.8) and phosphate (131.4) elements. It may be concluded from this investigation that therapy of MDR bacteria, hyperglycemia, and proteinuria can decrease urinary stone formation in diabetic patients by having UTIs.

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Effects of Glucose, Metformin, and Protein on Formation of Flower-like Nanocomposites of Struvite in Infected Artificial Urine Medium by Methicillin-resistant Staphylococcus aureus (MRSA): New Report

Show Author's information Hide Author's Information Nadia Asadi^¹, Mojtaba Taran^{²^,³}, Maryam Rad^², Mehran Alavi^{²^,³}(

)

Nephrology Department, Kermanshah University of Medicinal Sciences, Kermanshah, Iran

Microbiology Department, Razi University, Kermanshah, Iran

Nanobiotechnology Department, Razi University, Kermanshah, Iran

Abstract

Keywords: Staphylococcus aureus, Metformin, Urinary stone disease, Struvite nanocomposites, Diabetic patients, Artificial urine medium

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Publication history

Received: 16 October 2018

Accepted: 12 March 2019

Published: 28 March 2019

Issue date: March 2019

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Acknowledgements

We would like to appreciate all colleagues of microbiology lab of Razi University as well as Imam Reza hospital of Kermanshah for supporting of this study.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.