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Nano Biomedicine and Engineering 2018, 10(4): 392-405 https://doi.org/10.5101/nbe.v10i4.p392-405
Research Article | Open Access | Issue | Published: 21 November 2018

Effect of the Concentration of NaBH4 and N2H4 as Reductant Agent on the Synthesis of Copper Oxide Nanoparticles and its Potential Antimicrobial Applications

Show Author's Information Maribel Guzman1( ),  Mariella Arcos2,  Jean Dille3,  Stéphane Godet3,  Céline Rousse4
Engineering Department, Pontifical Catholic University of Peru, Av. Universitaria 1801, Lima-32, Peru
Sciences Department, Pontifical Catholic University of Peru, Av. Universitaria 1801, Lima-32, Peru
4MAT, Université Libre de Bruxelles, CP 194/03, 50 Avenue Roosevelt, B-1050 Brussels, Belgium
LISM, EA 4695, UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, BP 1039, 51687 Reims cedex 2, France
Keywords:
Antibacterial activity, Nanotechnology, Chemical reduction, Copper oxide nanoparticles, Staphylococcus aureus
Cite this article:
Guzman M, Arcos M, Dille J, et al. Effect of the Concentration of NaBH4 and N2H4 as Reductant Agent on the Synthesis of Copper Oxide Nanoparticles and its Potential Antimicrobial Applications. Nano Biomedicine and Engineering, 2018, 10(4): 392-405. https://doi.org/10.5101/nbe.v10i4.p392-405
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Abstract Full text About this article

The antibacterial activity of various nanoparticles is gaining increasing interest due to its potential medical applications. In this work, we presented the synthesis of copper oxide nanoparticles prepared by chemical reduction from aqueous solutions of copper sulfate (CuSO4) with sodium borohydride (NaBH4) and hydrazine hydrate (N2H4) as reductant and polyvinylpyrrolidone (PVP) as stabilizer. The X-ray diffraction spectra showed the formation of tenorite (CuO) and cuprite (Cu2O) nanoparticles when different ratios of CuSO4/NaBH4 and CuSO4/N2H4 were used. Photographs obtained by transmission electron microscopy (TEM) showed agglomerates of grains with a narrow size distribution (from 20 to 70 nm), whereas the radii of the individual particles were between 2 and 20 nm. Smaller nanoparticles and narrower particle size distributions were obtained when NaBH4 was used. The results of antibacterial activity using the Kirby-Bauer method showed that nanoparticles obtained with NaBH4 presented a reasonable bactericidal activity. Pseudomonas aureginosa and Staphylococcus aureus were more susceptible to the particle size than Escherichia coli. In addition, with small amounts of Cu2O in samples of CuO nanoparticles, the antibacterial susceptibility against Pseudomonas aureginosa was improved. Finally, nanoparticles of CuO incorporated into cotton by applying ultrasound waves remained impregnated after five washes.


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About this article

Effect of the Concentration of NaBH4 and N2H4 as Reductant Agent on the Synthesis of Copper Oxide Nanoparticles and its Potential Antimicrobial Applications

Show Author's information Maribel Guzman1( ), Mariella Arcos2, Jean Dille3, Stéphane Godet3, Céline Rousse4
Engineering Department, Pontifical Catholic University of Peru, Av. Universitaria 1801, Lima-32, Peru
Sciences Department, Pontifical Catholic University of Peru, Av. Universitaria 1801, Lima-32, Peru
4MAT, Université Libre de Bruxelles, CP 194/03, 50 Avenue Roosevelt, B-1050 Brussels, Belgium
LISM, EA 4695, UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, BP 1039, 51687 Reims cedex 2, France

Abstract

The antibacterial activity of various nanoparticles is gaining increasing interest due to its potential medical applications. In this work, we presented the synthesis of copper oxide nanoparticles prepared by chemical reduction from aqueous solutions of copper sulfate (CuSO4) with sodium borohydride (NaBH4) and hydrazine hydrate (N2H4) as reductant and polyvinylpyrrolidone (PVP) as stabilizer. The X-ray diffraction spectra showed the formation of tenorite (CuO) and cuprite (Cu2O) nanoparticles when different ratios of CuSO4/NaBH4 and CuSO4/N2H4 were used. Photographs obtained by transmission electron microscopy (TEM) showed agglomerates of grains with a narrow size distribution (from 20 to 70 nm), whereas the radii of the individual particles were between 2 and 20 nm. Smaller nanoparticles and narrower particle size distributions were obtained when NaBH4 was used. The results of antibacterial activity using the Kirby-Bauer method showed that nanoparticles obtained with NaBH4 presented a reasonable bactericidal activity. Pseudomonas aureginosa and Staphylococcus aureus were more susceptible to the particle size than Escherichia coli. In addition, with small amounts of Cu2O in samples of CuO nanoparticles, the antibacterial susceptibility against Pseudomonas aureginosa was improved. Finally, nanoparticles of CuO incorporated into cotton by applying ultrasound waves remained impregnated after five washes.

Keywords: Antibacterial activity, Nanotechnology, Chemical reduction, Copper oxide nanoparticles, Staphylococcus aureus

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Publication history

Received: 01 April 2018
Accepted: 03 September 2018
Published: 21 November 2018
Issue date: December 2018

Copyright

© Maribel Guzman, Mariella Arcos, Jean Dille, Stéphane Godet, and Céline Rousse.

Acknowledgements

Miss Maribel Guzman wants to thank the Laboratory of Biology of the University Cayetano Heredia from Perú, for the performed tests of antibacterial activities. This work was supported by the Pontifical Catholic University of Peru (20120098).

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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