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Nano Biomedicine and Engineering 2018, 10(2): 141-148 https://doi.org/10.5101/nbe.v10i2.p141-148
Research Article | Open Access | Issue | Published: 17 May 2018

Green Synthesis of Piperine/Triton X-100/Silver Nanoconjugates: Antimicrobial Activity and Cytotoxicity

Show Author's Information Muthusamy Prabakaran1,  Vellaikannu Kalaiarasi2,  Periakaruppan Nithya1,  Mani Gajendiran3( ),  Susaimanickam Arul Antony1( )
PG and Research Department of Chemistry, Presidency College, Chennai-600005, India
Department of Chemistry, Karpaga Vinayaga College of Engineering and Technology, Palayanoor, Maduranthakam, Chennai- 603308, India
Department of Chemistry, Vels University (VISTAS), Pallavaram, Chennai-600117, India
Keywords:
Antibacterial activity, Silver nanoparticles, Green synthesis, Piperine, Triton X-100
Cite this article:
Prabakaran M, Kalaiarasi V, Nithya P, et al. Green Synthesis of Piperine/Triton X-100/Silver Nanoconjugates: Antimicrobial Activity and Cytotoxicity. Nano Biomedicine and Engineering, 2018, 10(2): 141-148. https://doi.org/10.5101/nbe.v10i2.p141-148
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Abstract Full text About this article

Silver nanoparticles (AgNPs) were synthesized through a green chemical approach using the piperine isolated from black pepper. The physicochemical properties of Triton X-100 coated silver nanoparticles (Triton X-100/Ag NPs) were well characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and energy dispersive X-ray spectroscopy (EDS). The TEM images confirmed the spherical shape of silver nanoparticles. The powder X-ray diffraction analysis revealed the silver nanoparticles exhibiting face-centered cubic (fcc) crystal structure with an average crystallite size of 15 nm. The cytotoxicity effects on HepG2 cells were also evaluated with a series of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. The effective toxic concentration of Triton X-100/piperine with silver nanoparticles was too low to damage HepG2 cells. The antibacterial activity results showed that the Triton X-100/piperine/AgNPs efficiently inhibited the two bacteria namely S. aureus and E. coli. The Triton X-100/piperine/AgNPs nanoconjugates were effective in inhibiting the growth of both gram-positive and gram-negative bacteria S. aureus and E. coli.


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About this article

Green Synthesis of Piperine/Triton X-100/Silver Nanoconjugates: Antimicrobial Activity and Cytotoxicity

Show Author's information Muthusamy Prabakaran1, Vellaikannu Kalaiarasi2, Periakaruppan Nithya1, Mani Gajendiran3( ), Susaimanickam Arul Antony1( )
PG and Research Department of Chemistry, Presidency College, Chennai-600005, India
Department of Chemistry, Karpaga Vinayaga College of Engineering and Technology, Palayanoor, Maduranthakam, Chennai- 603308, India
Department of Chemistry, Vels University (VISTAS), Pallavaram, Chennai-600117, India

Abstract

Silver nanoparticles (AgNPs) were synthesized through a green chemical approach using the piperine isolated from black pepper. The physicochemical properties of Triton X-100 coated silver nanoparticles (Triton X-100/Ag NPs) were well characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and energy dispersive X-ray spectroscopy (EDS). The TEM images confirmed the spherical shape of silver nanoparticles. The powder X-ray diffraction analysis revealed the silver nanoparticles exhibiting face-centered cubic (fcc) crystal structure with an average crystallite size of 15 nm. The cytotoxicity effects on HepG2 cells were also evaluated with a series of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. The effective toxic concentration of Triton X-100/piperine with silver nanoparticles was too low to damage HepG2 cells. The antibacterial activity results showed that the Triton X-100/piperine/AgNPs efficiently inhibited the two bacteria namely S. aureus and E. coli. The Triton X-100/piperine/AgNPs nanoconjugates were effective in inhibiting the growth of both gram-positive and gram-negative bacteria S. aureus and E. coli.

Keywords: Antibacterial activity, Silver nanoparticles, Green synthesis, Piperine, Triton X-100

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Publication history

Received: 30 March 2017
Accepted: 10 May 2018
Published: 17 May 2018
Issue date: June 2018

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© Muthusamy Prabakaran, Vellaikannu Kalaiarasi, Periakaruppan Nithya, Mani Gajendiran, and Susaimanickam Arul Antony.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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