Journal Home > Volume 10 , Issue 1
Nano Biomedicine and Engineering 2018, 10(1): 25-33 https://doi.org/10.5101/nbe.v10i1.p25-33
Research Article | Open Access | Issue | Published: 28 February 2018

Effect of Incubation Time, CuSO4 and Glucose Concentrations on Biosynthesis of Copper Oxide (CuO) Nanoparticles with Rectangular Shape and Antibacterial Activity: Taguchi Method Approach

Show Author's Information Maryam Rad1,  Mojtaba Taran1,  Mehran Alavi2( )
Department of Biology, Faculty of Science, Razi University P.O. Box, 6714967346, Kermanshah, Iran
Department of Nanobiotechnology, Faculty of Science, Razi University P.O. Box, 6714967346, Kermanshah, Iran
Keywords:
Biosynthesis, CuO nanoparticles, Halomonas elongata IBRC-M 10214, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 43300
Cite this article:
Rad M, Taran M, Alavi M. Effect of Incubation Time, CuSO4 and Glucose Concentrations on Biosynthesis of Copper Oxide (CuO) Nanoparticles with Rectangular Shape and Antibacterial Activity: Taguchi Method Approach. Nano Biomedicine and Engineering, 2018, 10(1): 25-33. https://doi.org/10.5101/nbe.v10i1.p25-33
Download citation
EndNote(RIS)
BibTeX

681

Views

37

Downloads

Citations

36

Crossref

N/A

WoS

46

Scopus

N/A

CSCD

Abstract Full text About this article

In nanotechnology, using of metallic nanoparticles (MNPs) has obtained major attention for over a century because of their unique properties in nano scale. In this paper, the effect of three factors (incubation time, concentrations of copper sulfate and glucose) on the biosynthesis of copper (Ⅱ) oxide nanoparticles (CuO NPs) by Halomonas elongata IBRC-M 10214 and the antibacterial activity of nanoparticles were evaluated. Experiment design and analysis of Taguchi method were carried out by Qualitek-4 software. Effect of CuSO4 concentration (1.4, 2.8 and 5.6 mM), incubation time (48, 72 and 96 h) as three different levels was measured as three major factors in the biosynthesis of metallic nanoparticles. Synthesized CuO nanoparticles were characterized by utilizing ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infra-red (FT-IR) spectroscopy and field emission scanning electron microscope (FESEM) techniques. Among the three factors, results illustrated that considerable effect was related to CuSO4 concentration. These analyses demonstrated that the average CuO nanoparticles crystalline size was 57-79 nm with rectangular shape. Also, for evaluating of antibacterial effects, maximum zone of inhibition, two important multidrug resistant pathogenesis bacteria, Escherichia coli ATCC 25922, and Staphylococcus aureus ATCC 43300 were used. Antibacterial assay of CuO nanoparticles showed antibacterial activity toward the pathogenic bacterial strains of E. coli by 5 mm and S. aureus by 5.5 mm for maximum zone of inhibition. In conclusion, this study presented simple, low expensive, eco-friendly and high productivity in the fabrication of CuO nanoparticles. In addition, these metallic nanoparticles had antibacterial effect that could be usable in medicinal aspect for fighting against prominent pathogen bacteria such as E. coli ATCC 25922 and S. aureus ATCC 43300.


menu
Abstract
Full text
Outline
About this article

Effect of Incubation Time, CuSO4 and Glucose Concentrations on Biosynthesis of Copper Oxide (CuO) Nanoparticles with Rectangular Shape and Antibacterial Activity: Taguchi Method Approach

Show Author's information Maryam Rad1, Mojtaba Taran1, Mehran Alavi2( )
Department of Biology, Faculty of Science, Razi University P.O. Box, 6714967346, Kermanshah, Iran
Department of Nanobiotechnology, Faculty of Science, Razi University P.O. Box, 6714967346, Kermanshah, Iran

Abstract

In nanotechnology, using of metallic nanoparticles (MNPs) has obtained major attention for over a century because of their unique properties in nano scale. In this paper, the effect of three factors (incubation time, concentrations of copper sulfate and glucose) on the biosynthesis of copper (Ⅱ) oxide nanoparticles (CuO NPs) by Halomonas elongata IBRC-M 10214 and the antibacterial activity of nanoparticles were evaluated. Experiment design and analysis of Taguchi method were carried out by Qualitek-4 software. Effect of CuSO4 concentration (1.4, 2.8 and 5.6 mM), incubation time (48, 72 and 96 h) as three different levels was measured as three major factors in the biosynthesis of metallic nanoparticles. Synthesized CuO nanoparticles were characterized by utilizing ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infra-red (FT-IR) spectroscopy and field emission scanning electron microscope (FESEM) techniques. Among the three factors, results illustrated that considerable effect was related to CuSO4 concentration. These analyses demonstrated that the average CuO nanoparticles crystalline size was 57-79 nm with rectangular shape. Also, for evaluating of antibacterial effects, maximum zone of inhibition, two important multidrug resistant pathogenesis bacteria, Escherichia coli ATCC 25922, and Staphylococcus aureus ATCC 43300 were used. Antibacterial assay of CuO nanoparticles showed antibacterial activity toward the pathogenic bacterial strains of E. coli by 5 mm and S. aureus by 5.5 mm for maximum zone of inhibition. In conclusion, this study presented simple, low expensive, eco-friendly and high productivity in the fabrication of CuO nanoparticles. In addition, these metallic nanoparticles had antibacterial effect that could be usable in medicinal aspect for fighting against prominent pathogen bacteria such as E. coli ATCC 25922 and S. aureus ATCC 43300.

Keywords: Biosynthesis, CuO nanoparticles, Halomonas elongata IBRC-M 10214, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 43300

References(64)

[1]

E. Blanco, H. Shen, and M. Ferrari, Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat Biotech, 2015, 33(9): 941-951.
DOI Google Scholar
[2]

M. Alavi, N. Karimi, and M. Safaei, Application of various types of liposomes in drug delivery systems. Advanced pharmaceutical bulletin, 2017, 7(1): 3-9.
DOI Google Scholar
[3]

B. Issa, I.M. Obaidat, B.A. Albiss, et al., Magnetic nanoparticles: surface effects and properties related to biomedicine applications. International Journal of Molecular Sciences, 2013, 14(11): 21266-21305.
DOI Google Scholar
[4]

M. Alavi, N. Karimi, Important factors in drug release rate of microspheres. Biological, Environmental and Agricultural Sciences, 2017, 1: 31-33.
Google Scholar
[5]

O.V. Salata, Applications of nanoparticles in biology and medicine. Journal of nanobiotechnology, 2004, 2: 3-3.
DOI Google Scholar
[6]

X.F. Zhang, Z.G. Liu, W. Shen, et al., Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. International Journal of Molecular Sciences, 2016, 17(9): 1534.
DOI Google Scholar
[7]

S.M. Ng, M. Koneswaran, and R. Narayanaswamy, A review on fluorescent inorganic nanoparticles for optical sensing applications. RSC Advances, 2016, 6: 21624-21661.
DOI Google Scholar
[8]

X. Wu, R.H. Guy, Applications of nanoparticles in topical drug delivery and in cosmetics. Journal of Drug Delivery Science and Technology, 2009, 19(6): 371-384.
DOI Google Scholar
[9]

A. Martirosyan, Y.J. Schneider, Engineered nanomaterials in food: Implications for food safety and consumer health. International Journal of Environmental Research and Public Health, 2014, 11(6): 5720-5750.
DOI Google Scholar
[10]

B.S. Sekhon, Food nanotechnology-an overview. Nanotechnology, Science and Applications, 2010, 3: 1-15.
DOI Google Scholar
[11]

A. Ali, H. Zafar, M. Zia, et al., Synthesis, characterization, applications, and challenges of iron oxide nanoparticles. Nanotechnology, Science and Applications, 2016, 19(9) 49-67.
DOI Google Scholar
[12]

V.V. Mody, R. Siwale, A. Singh, et al., Introduction to metallic nanoparticles. Journal of Pharmacy and Bioallied Sciences, 2010, 2(4): 282-289.
DOI Google Scholar
[13]

A.M. Eremenko, I.S. Petrik, N.P. Smirnova, et al., Antibacterial and antimycotic activity of cotton fabrics, impregnated with silver and binary silver/copper nanoparticles. Nanoscale Research Letters, 2016, 11: 28.
DOI Google Scholar
[14]

A. Yabuki, N. Arriffin, Electrical conductivity of copper nanoparticle thin films annealed at low temperature. Thin Solid Films, 2010, 518(23): 7033-7037.
DOI Google Scholar
[15]

M. Saterlie, H. Sahin, B. Kavlicoglu, et al., Particle size effects in the thermal conductivity enhancement of copper-based nanofluids. Nanoscale Research Letters, 2011, 6(1): 217-217.
DOI Google Scholar
[16]

J. Ramyadevi, K. Jeyasubramanian, A. Marikani, et al., Synthesis and antimicrobial activity of copper nanoparticles. Materials Letters, 2012, 71: 114-116.
DOI Google Scholar
[17]

J. P. Ruparelia, A.K. Chatterjee, S.P. Duttagupta, et al., Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomaterialia, 2008, 4(3): 707-716.
DOI Google Scholar
[18]

O.V. Zakharova, A.Y. Godymchuk, A.A. Gusev, et al., Considerable variation of antibacterial activity of Cu nanoparticles suspensions depending on the storage time, dispersive medium, and particle sizes. BioMed Research International, 2015, 2015: 412530.
DOI Google Scholar
[19]

M.S. Usman, M.E.E. Zowalaty, K. Shameli, et al., Synthesis, characterization, and antimicrobial properties of copper nanoparticles. International Journal of Nanomedicine, 2013, 8: 4467-4479.
DOI Google Scholar
[20]

R. Betancourt-Galindo, P.Y. Reyes-Rodriguez, B.A. Puente-Urbina, et al., Synthesis of copper nanoparticles by thermal decomposition and their antimicrobial properties. Journal of Nanomaterials, 2014, 2014: 1-5.
DOI Google Scholar
[21]

B.L. Cushing, V.L. Kolesnichenko, and C.J. O'Connor, Recent advances in the liquid-phase syntheses of inorganic nanoparticles. Chemical Reviews, 2004, 104: 3893-3946.
DOI Google Scholar
[22]

E. Ayoman, G. Hossini, and N. Haghighi, synthesis of CuO nanoparticles and study on their catalytic properties. International Journal of Nanoscience and Nanotechnology, 2015, 11: 63-70.
Google Scholar
[23]

S. Stankic, S. Suman, F. Haque, et al., Pure and multi metal oxide nanoparticles: synthesis, antibacterial and cytotoxic properties. Journal of Nanobiotechnology, 2016, 14: 73.
DOI Google Scholar
[24]

N. Kulkarni, U. Muddapur, Biosynthesis of metal nanoparticles: A review. Journal of Nanotechnology, 2014, 2014: 1-8.
DOI Google Scholar
[25]

M. Taran, M. Rad, and M. Alavi, Characterization of Ag nanoparticles biosynthesized by Bacillus sp. HAI4 in different conditions and their antibacterial effects. Journal of Applied Pharmaceutical Science, 2016, 6: 094-099.
DOI Google Scholar
[26]

S. Khaghani, D. Ghanbari, and S. Khaghani, Green synthesis of iron oxide-palladium nanocomposites by pepper extract and its application in removing of colored pollutants from water. Journal of Nanostructures, 2017, 7: 175-182.
DOI Google Scholar
[27]

M. Alavi, N. Karimi, Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract. Artificial Cells, Nanomedicine, and Biotechnology, 2017, 12: 1-16.
DOI Google Scholar
[28]

R. Varshney, S. Bhadauria, and M.S. Gaur, A review: Biological synthesis of silver and copper nanoparticles. Nano Biomedicine and Engineering, 2012, 4(2): 99-106.
DOI Google Scholar
[29]

S. Ahmed, M. Ahmad, B.L. Swami, et al., A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. Journal of Advanced Research, 2016, 7: 17-28.
DOI Google Scholar
[30]

M. Balamurugan, S. Kaushik, and S. Saravanan, Green synthesis of gold nanoparticles by using Peltophorum pterocarpum flower extracts. Nano Biomedicine and Engineering, 2016, 8: 213-218.
DOI Google Scholar
[31]

S.A. Aromal, D. Philip, Green synthesis of gold nanoparticles using Trigonella foenum-graecum and its size-dependent catalytic activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2012, 97: 1-5.
DOI Google Scholar
[32]

N. Saifuddin, C.W. Wong, and A.A. Yasumira, Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation. Journal of Chemistry, 2009, 6: 61-70.
DOI Google Scholar
[33]

M. Taran, M. Rad, and M. Alavi, Biological synthesis of copper nanoparticles by using Halomonas elongata IBRC-M 10214. Industria Textila, 2016, 67: 351.
Google Scholar
[34]

V.G. Debabov, T.A. Voeikova, A.S. Shebanova, et al., Bacterial synthesis of silver sulfide nanoparticles. Nanotechnologies in Russia, 2013, 8: 269-276.
DOI Google Scholar
[35]

M. Oves, M.S. Khan, A. Zaidi, et al., Antibacterial and cytotoxic efficacy of extracellular silver nanoparticles biofabricated from chromium reducing novel OS4 strain of Stenotrophomonas maltophilia. PloS One, 2013, 8: e59140.
DOI Google Scholar
[36]

F.M. Morsy, N.A. Nafady, M.H. Abd-Alla, et al., Green synthesis of silver nanoparticles by water soluble fraction of the extracellular polysaccharides/matrix of the cyanobacterium Nostoc commune and its application as a potent fungal surface sterilizing agent of seed crops. Universal Journal of Microbiology Research, 2014, 2: 36-43.
DOI Google Scholar
[37]

R. Singh, U.U. Shedbalkar, S.A. Wadhwani, et al., Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Applied Microbiology and Biotechn ology, 2015, 99: 4579-4593.
DOI Google Scholar
[38]

M. Taran, E. Azizi, S. Taran, et al., Archaeal poly (3-hydroxybutyrate) polymer production from glycerol: Optimization by Taguchi methodology. Journal of Polymers and the Environment, 2011, 19: 750-754.
DOI Google Scholar
[39]

M. Taran, M. Rad, and M. Alavi, Biosynthesis of TiO2 and ZnO nanoparticles by Halomonas elongata IBRC-M 10214 in different conditions of medium. Bioimpacts, 2018, 8: 65-74.
DOI Google Scholar
[40]

M. Taran, M. Sharifi, and S. Bagheri, Utilization of textile wastewater as carbon source by newly isolated Haloarcula sp. IRU1: optimization of conditions by Taguchi methodology. Clean Technologies and Environmental Policy, 2011, 13: 535-538.
DOI Google Scholar
[41]

M. Taran, Utilization of petrochemical wastewater for the production of poly(3-hydroxybutyrate) by Haloarcula sp. IRU1. Journal of Hazardous Materials, 2011, 188, 26-28.
DOI Google Scholar
[42]

M. Taran, M. Safaei, Optimal conditions for producing bactericidal sodium hyaluronate-TiO2 bionanocomposite and its characterization. International Journal of Biological Macromolecules, 2017, 104: 449-456.
DOI Google Scholar
[43]

M. Rodríguez-Sáiz, C. Sánchez-Porro, J.L. De La Fuente, et al., Engineering the halophilic bacterium Halomonas elongata to produce β-carotene. Applied Microbiology and Biotechnology, 2007, 77: 637-643.
DOI Google Scholar
[44]

D. Canovas, C. Vargas, L.N. Csonka, et al., Osmoprotectants in Halomonas elongata : high-affinity betaine transport system and choline-betaine pathway. Journal of Bacteriology, 1996, 178: 7221-7226.
DOI Google Scholar
[45]

S.S. Hasan, S. Singh, R.Y. Parikh, et al., Bacterial synthesis of copper/copper oxide nanoparticles. Journal of Nanoscience and Nanotechnology, 2008, 8: 3191-3196.
DOI Google Scholar
[46]

R. Chauhan, J. Abraham, In vitro antimicrobial potential of the lichen Parmotrema sp. extracts against various pathogens. Iranian Journal of Basic Medical Sciences, 2013, 16: 882-885.
Google Scholar
[47]

F. Lotfipour, H. Nazemiyeh, F. Fathi-Azad, et al., Evaluation of Antibacterial Activities of Some Medicinal Plants from North-West Iran. Iranian Journal of Basic Medical Sciences, 2008, 11: 80-85.
Google Scholar
[48]

L. Yadav, R.M. Tripathi, R. Prasad, et al., Antibacterial activity of Cu nanoparticles against E. coli, Staphylococcus aureus and Pseudomonas aeruginosa. Nano Biomedicine and Engineering, 2017, 9: 9-14.
DOI Google Scholar
[49]

D.R. Baer, M.H. Engelhard, G.E. Johnson, et al., Surface characterization of nanomaterials and nanoparticles: Important needs and challenging opportunities. Journal of Vacuum Science & Technology. A, Vacuum, Surfaces, and Films: An Official Journal of the American Vacuum Society, 2013, 31: 050820.
DOI Google Scholar
[50]

S. Shende, A.P. Ingle, A. Gade, et al., Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity. World Journal of Microbiology & Biotechnology, 2015, 31: 865-873.
DOI Google Scholar
[51]

W.H. Yang, Y.S. Tarng, Design optimization of cutting parameters for turning operations based on the Taguchi method. Journal of Materials Processing Technology, 1998, 84: 122-129.
DOI Google Scholar
[52]

S. Tang, H.Y.J. Tan, S.M. Sapuan, et al., The use of Taguchi method in the design of plastic injection mould for reducing warpage. Journal of Materials Processing Technology, 2007, 182: 418-426.
DOI Google Scholar
[53]

M. Taran, A. Monazah, and M. Alavi, Using petrochemical wastewater for synthesis of cruxrhodopsin as an energy capturing nanoparticle by Haloarcula sp. IRU1. Progress in Biological Sciences, 2017, 6: 151-157.
Google Scholar
[54]

D. Dorjnamjin, M. Ariunaa, Y.K. Shim, Synthesis of Silver nanoparticles using hydroxyl functionalized ionic liquids and their antimicrobial activity. International Journal of Molecular Sciences, 2008, 9: 807-820.
DOI Google Scholar
[55]

C.G. Kumar, S.K. Mamidyala, Extracellular synthesis of silver nanoparticles using culture supernatant of Pseudomonas aeruginosa. Colloids and Surfaces. B, Biointerfaces, 2011, 84: 462-466.
DOI Google Scholar
[56]

P.K. Khanna, P. More, J. Jawalkar, et al., Synthesis of hydrophilic copper nanoparticles: effect of reaction temperature. Journal of Nanoparticle Research, 2009, 11: 793-799.
DOI Google Scholar
[57]

Y.H. Kim, D.K. Lee, H.G. Cha, et al., Preparation and characterization of the antibacterial Cu nanoparticle formed on the surface of SiO2 nanoparticles. The Journal of Physical Chemistry. B, 2006, 110: 24923-24928.
DOI Google Scholar
[58]

A. Berendjchi, R. Khajavi, M.E. Yazdanshenas, Fabrication of superhydrophobic and antibacterial surface on cotton fabric by doped silica- based sols with nanoparticles of copper. Nanoscale Research Letters, 2011, 6: 594.
DOI Google Scholar
[59]

G. Ren, D. Hu, E.W.C. Cheng, et al., Characterisation of copper oxide nanoparticles for antimicrobial applications. International Journal of Antimicrobial Agents, 2009, 33: 587-590.
DOI Google Scholar
[60]

T. Kruk, K. Szczepanowicz, J. Stefańska, et al., Synthesis and antimicrobial activity of monodisperse copper nanoparticles. Colloids and Surfaces B: Biointerfaces, 2015, 128, 17-22.
DOI Google Scholar
[61]

W. Shao, X. Liu, H. Min, et al., Preparation, characterization, and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite. ACS Applied Materials & Interfaces, 2015, 7: 6966-6973.
DOI Google Scholar
[62]

J. Chen, H. Peng, X. Wang, et al., Graphene oxide exhibits broad-spectrum antimicrobial activity against bacterial phytopathogens and fungal conidia by intertwining and membrane perturbation. Nanoscale, 2014, 6: 1879-1889.
DOI Google Scholar
[63]

I. Sondi, B. Salopek-Sondi, Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science, 2004, 275: 177-182.
DOI Google Scholar
[64]

A.M. El Badawy, R.G. Silva, B. Morris, et al., Surface charge-dependent toxicity of silver nanoparticles. Environmental Science & Technology, 2010, 45: 283-287.
DOI Google Scholar
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 08 December 2017
Accepted: 26 February 2018
Published: 28 February 2018
Issue date: March 2018

Copyright

© Maryam Rad, Mojtaba Taran, and Mehran Alavi.

Acknowledgements

Acknowledgements

The authors wish to appreciate Razi University for providing necessary facilities to carry out this work.

Rights and permissions

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Return