Effects of modified atmosphere with nitrogen on physiological and biochemical responses of Tribolium castaneum

In order to determine the effects of different nitrogen volume fractions on physiological and biochemical responses of Tribolium castaneum, the changes of total superoxide dismutase(SOD)activity, peroxidase (POD) activity, catalase (CAT) activity, total antioxidant capacity (T-AOC) and lactic acid (LD) content in larvae and adults of T. castaneum were investigated at 28℃±1℃, RH 75%±5%, nitrogen volume fractions of (78% (atmospheric pressure as the control), 90%, 95%, and 98%), different treatment time (2 d, 4 d, 6 d, and 8 d). The results showed that with the increase of nitrogen volume fraction, SOD, POD and CAT activities in the larvae and adults of T. castaneum were the fastest when the treatment time was 8 days, and the activities of SOD, POD and CAT in the larvae and adults decreased to 0 when the nitrogen volume fraction was 98% for 8 d, and all the larvae and adults died. The T-AOC value in the larvae increased with increasing nitrogen volume fraction, and the response rate was the fastest at 6 d and 8 d, while the T-AOC value decreased to 0 when the nitrogen volume fraction was 98% at 6 d and 8 d. The response rate of T-AOC in adults was the highest when the treatment time was 8 d, and the T-AOC of the larvae decreased to 0 when the nitrogen volume fraction was 98% for 8 d. The LD content in the larvae increased with increasing nitrogen volume fraction, and the response rate was the fastest at 6 d and 8 d, while the LD content in the larvae decreased to 0 at 6 d and 8 d when the nitrogen volume fraction was 98%. The response rate of LD content of the adults was the fastest when the treatment time was 8 d, and the LD content decreased to 0 when the nitrogen volume fraction was 98% for 8 d.