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Alcohol has many effects on brain function and human behavior, inducing anxiety and sedation, motor incoordination, vomiting, hypnosis, and ultimately unconsciousness. Motor function, in particular, is affected by acute and chronic exposure to alcohol, as several studies have shown. However, the role of the primary motor cortex (M1), a major component of sensorimotor integration, in alcohol-induced motor injury is still unclear. For mouse model of acute alcohol exposure, we intraperitoneally injected alcohol in a single high dose (3 g/kg) and observed the effects of alcohol exposure on neuronal c-Fos expression in mouse brains and behavioral changes in mice. We found significantly decreased locomotion coupled with significantly increased neuronal activation selectively in M1 24 h after injection of alcohol, but anxiety level was not affected. Our findings contribute to the understanding of the association between M1 function and alcohol and open a possible path for early intervention in the treatment of alcohol use symptoms.


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Acute alcohol exposure suppressed locomotor activity in mice

Show Author's information Ke Zhang1,§Ruo-Fan Li1,§Han Li1,§He Lin2Zhe-Ming Sun2Shu-Lu Zhan1( )
Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
The Third Research Institute of Ministry of Public Security, Shanghai 200031, China

§ Ke Zhang, Ruo-Fan Li, and Han Li contributed equally to this work.

Abstract

Alcohol has many effects on brain function and human behavior, inducing anxiety and sedation, motor incoordination, vomiting, hypnosis, and ultimately unconsciousness. Motor function, in particular, is affected by acute and chronic exposure to alcohol, as several studies have shown. However, the role of the primary motor cortex (M1), a major component of sensorimotor integration, in alcohol-induced motor injury is still unclear. For mouse model of acute alcohol exposure, we intraperitoneally injected alcohol in a single high dose (3 g/kg) and observed the effects of alcohol exposure on neuronal c-Fos expression in mouse brains and behavioral changes in mice. We found significantly decreased locomotion coupled with significantly increased neuronal activation selectively in M1 24 h after injection of alcohol, but anxiety level was not affected. Our findings contribute to the understanding of the association between M1 function and alcohol and open a possible path for early intervention in the treatment of alcohol use symptoms.

Keywords:

alcohol exposure, locomotion, primary motor cortex, anxiety
Received: 02 May 2022 Revised: 04 July 2022 Accepted: 12 July 2022 Published: 28 July 2022 Issue date: June 2022
References(16)
[1]
Carvalho, A. F., Heilig, M., Perez, A., Probst, C., Rehm, J. Alcohol use disorders. Lancet, 2019, 394(10200): 781–792.
[2]
Global status report on alcohol and health 2018. World Health Organization, 2018.
[3]
Harrison, N. L., Skelly, M. J., Grosserode, E. K., Lowes, D. C., Zeric, T., Phister, S., Salling, M. C. Effects of acute alcohol on excitability in the CNS. Neuropharmacology, 2017, 122: 36–45.
[4]
Smoothy, R., Berry, M. S. Alcohol increases both locomotion and immobility in mice: An ethological analysis of spontaneous motor activity. Psychopharmacology, 1984, 83(3): 272–276.
[5]
Philibin, S. D., Cameron, A. J., Schlumbohm, J. P., Metten, P., Crabbe, J. C. Ethanol withdrawal-induced motor impairment in mice. Psychopharmacology, 2012, 220(2): 367–378.
[6]
Karadayian, A. G., Cutrera, R. A. Alcohol hangover: Type and time-extension of motor function impairments. Behavioural Brain Research, 2013, 247: 165–173.
[7]
Kril, J. J., Halliday, G. M., Svoboda, M. D., Cartwright, H. The cerebral cortex is damaged in chronic alcoholics. Neuroscience, 1997, 79(4): 983–998.
[8]
Abernathy, K., Chandler, L. J., Woodward, J. J. Alcohol and the prefrontal cortex. International Review of Neurobiology, 2010, 91: 289–320.
[9]
Kähkönen, S., Kesäniemi, M., Nikouline, V. V., Karhu, J., Ollikainen, M., Holi, M., Ilmoniemi, R. J. Ethanol modulates cortical activity: Direct evidence with combined TMS and EEG. NeuroImage, 2001, 14(2): 322–328.
[10]
Kähkönen, S., Wilenius, J., Nikulin, V. V., Ollikainen, M., Ilmoniemi, R. J. Alcohol reduces prefrontal cortical excitability in humans: a combined TMS and EEG study. Neuropsychopharmacology, 2003, 28(4): 747–754.
[11]
Campbell, A. E., Sumner, P., Singh, K. D., Muthukumaraswamy, S. D. Acute effects of alcohol on stimulus-induced gamma oscillations in human primary visual and motor cortices. Neuropsychopharmacology, 2014, 39(9): 2104–2113.
[12]
Premoli, I., Castellanos, N., Rivolta, D., Belardinelli, P., Bajo, R., Zipser, C., Espenhahn, S., Heidegger, T., Müller-Dahlhaus, F., Ziemann, U. TMS-EEG signatures of GABAergic neurotransmission in the human cortex. Journal of Neuroscience, 2014, 34(16): 5603–5612.
[13]
Amodeo, L. R., Jennings, S. D., Mulholland, P. J., Ehlers, C. L. Chronic intermittent ethanol during adolescence and adulthood alters dendritic spines in the primary motor and visual cortex in rats. Alcohol, 2021, 97: 67–74.
[14]
Peng, S.-Y., Shi, Z., Zhou, D.-S., Wang, X.-Y., Li, X.-X., Liu, X.-L., Wang, W.-D., Lin, G.-N., Pan, B.-X., Voon, V., Grace, A. A., Heilig, M., Wong, M.-L., Yuan, T.-F. Reduced motor cortex GABA(B)R function following chronic alcohol exposure. Molecular Psychiatry, 2021, 26(2): 383–395.
[15]
Vogt, B. L., Richie, J. P. Glutathione depletion and recovery after acute ethanol administration in the aging mouse. Biochemical Pharmacology, 2007, 73(10): 1613–1621.
[16]
Costardi, J. V. V., Nampo, R. A. T., Silva, G. L., Ribeiro, M. A. F., Stella, H. J., Stella, M. B., Malheiros, S. V. P. A review on alcohol: From the central action mechanism to chemical dependency. Revista Da Associacao Medica Brasileira (1992), 2015, 61(4): 381–387.
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Publication history

Received: 02 May 2022
Revised: 04 July 2022
Accepted: 12 July 2022
Published: 28 July 2022
Issue date: June 2022

Copyright

© The Author(s) 2022

Acknowledgements

We thank all the members of the lab for their help. K.Z. performed the experiment. R.F.L. and L.H. analyzed the data. All of the authors drafted the manuscript together.

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Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attributtion-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission.

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