Evaluation of the Antibacterial Impact of the Conjugated Gentamycin-biosynthesized Silver Nanoparticles on Resistant <i>Pseudomonas aeruginosa</i> Isolates <i>In Vitro</i>

Dalal Mohammed Ridha; Hawraa Mohammed AL-Rafyai; Mustafa Abed AL-Jabber Mohammed Saleh

doi:10.26599/NBE.2024.9290067

Nano Biomedicine and Engineering https://doi.org/10.26599/NBE.2024.9290067

Research Article |

Open Access | Online first | Published: 22 March 2024

Evaluation of the Antibacterial Impact of the Conjugated Gentamycin-biosynthesized Silver Nanoparticles on Resistant Pseudomonas aeruginosa Isolates In Vitro

Show Author's Information Hide Author's Information Dalal Mohammed Ridha^¹(

), Hawraa Mohammed AL-Rafyai^¹, Mustafa Abed AL-Jabber Mohammed Saleh^²

1Biology Department, Science faculty, Babylon University, Iraq

2Medical Laboratory Techniques Department, Hilla University College, Babylon, Iraq

Keywords:

antibacterial activity, Pseudomonas aeruginosa, gentamycin (GEN), multidrug-resistant strains, biosynthesized silver nanoparticles (Bio-AgNPs)_.

Cite this article:

Ridha DM, AL-Rafyai HM, Saleh MAA-JM. Evaluation of the Antibacterial Impact of the Conjugated Gentamycin-biosynthesized Silver Nanoparticles on Resistant Pseudomonas aeruginosa Isolates In Vitro. Nano Biomedicine and Engineering, 2024, https://doi.org/10.26599/NBE.2024.9290067

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Abstract

The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health. Thus, a new strategy is required to resolve this problem. This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles (Bio-AgNPs), gentamycin (GEN), and the conjugation of biosynthesized silver nanoparticles and gentamycin (GEN:Bio-AgNPs) on multidrug-resistant Pseudomonas aeruginosa isolates. The characteristic properties of Bio-AgNPs were detected by the following analyses: ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Depending on micro-dilution assay, the minimum inhibitory concentration (MIC) of the tested subjects (Bio-AgNPs, GEN, GEN:Bio-AgNPs) are calculated to be 112 ± 400, 1 536 ± 525, and 49 ± 18.8 µg/mL, respectively. The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone, showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P. aeruginosa.

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Evaluation of the Antibacterial Impact of the Conjugated Gentamycin-biosynthesized Silver Nanoparticles on Resistant Pseudomonas aeruginosa Isolates In Vitro

Show Author's information Hide Author's Information Dalal Mohammed Ridha^¹(

), Hawraa Mohammed AL-Rafyai^¹, Mustafa Abed AL-Jabber Mohammed Saleh^²

1Biology Department, Science faculty, Babylon University, Iraq

2Medical Laboratory Techniques Department, Hilla University College, Babylon, Iraq

Abstract

Keywords: antibacterial activity, Pseudomonas aeruginosa, gentamycin (GEN), multidrug-resistant strains, biosynthesized silver nanoparticles (Bio-AgNPs)_.

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Received: 06 October 2023

Revised: 24 November 2023

Accepted: 15 December 2023

Published: 22 March 2024

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This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY) (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.