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Research Article | Open Access

Silver Nanoparticles Synthesized by Cold Plasma as an Antibiofilm Agent against Staphylococcus epidermidis Isolated from Acne

Heba Khaleel Tawfeeq1Nisreen Kh. Abdalameer2( )Rafal H. Jassim2Maryam M. Shehab1
Department of Biology/ College of Science/ University of Baghdad, Baghdad, Iraq
Department of Physics/ College of Science for Women/ University of Baghdad/ Baghdad, Iraq
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Graphical Abstract

Abstract

This study aimed to investigate the efficacy of silver nanoparticles (AgNPs) synthesized by cold plasma in inhibiting biofilm formed by Staphylococcus epidermidis both phenotypically and genotypically. The resultant AgNPs were characterized by using different parameters, color change, X-ray diffraction (XRD), zeta potential, feld emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM) methods. Moreover, their efficacy in inhibiting S. epidermidis biofilm was studied by the microtiter plate method. The results revealed that cold plasma succeeded in the synthesis of AgNPs with a size range of 10–20 nm. Markedly, the present findings indicated a significant decrease (P < 005) in the intensity of all tested biofilm alongside the down-regulation of accumulation-associated protein (aap) and AtlE in the presence of AgNPs as compared with non-treated cells. In conclusion, AgNPs synthesized by cold plasma acted as antibiofilm agents and could be used for pharmaceutical applications.

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Nano Biomedicine and Engineering
Pages 101-109
Cite this article:
Tawfeeq HK, Abdalameer NK, Jassim RH, et al. Silver Nanoparticles Synthesized by Cold Plasma as an Antibiofilm Agent against Staphylococcus epidermidis Isolated from Acne. Nano Biomedicine and Engineering, 2024, 16(1): 101-109. https://doi.org/10.26599/NBE.2023.9290042

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Received: 11 August 2023
Revised: 08 September 2023
Accepted: 26 September 2023
Published: 17 November 2023
© The Author(s) 2024.

This is an open-access article distributed under  the  terms  of  the  Creative  Commons  Attribution  4.0 International  License (CC BY) (http://creativecommons.org/licenses/by/4.0/), which  permits  unrestricted  use,  distribution,  and reproduction in any medium, provided the original author and source are credited.

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