Biosynthesis of CuO Nanoparticles Using Plant Extract as a Precursor: Characterization, Antibacterial, and Antioxidant Activity

Pawar Abhimanyu; Mungole Arvind; Naktode Kishor

doi:10.26599/NBE.2023.9290027

Nano Biomedicine and Engineering 2023, 15(4): 369-377 https://doi.org/10.26599/NBE.2023.9290027

Research Article |

Open Access | Issue | Published: 21 August 2023

Biosynthesis of CuO Nanoparticles Using Plant Extract as a Precursor: Characterization, Antibacterial, and Antioxidant Activity

Show Author's Information Hide Author's Information Pawar Abhimanyu^¹(

), Mungole Arvind^², Naktode Kishor^¹

1Department of Chemistry, NevjabaiHitkarini College, Bramhapuri, Maharashtra 441 206, India

2Department of Botany, NevjabaiHitkarini College, Bramhapuri, Maharashtra 441 206, India

Keywords:

X-ray diffraction (XRD), transmission electron microscopy (TEM), antioxidant activity, antimicrobial activity, CuO nanoparticles (NPs)

Cite this article:

Abhimanyu P, Arvind M, Kishor N. Biosynthesis of CuO Nanoparticles Using Plant Extract as a Precursor: Characterization, Antibacterial, and Antioxidant Activity. Nano Biomedicine and Engineering, 2023, 15(4): 369-377. https://doi.org/10.26599/NBE.2023.9290027

Download citation

EndNote(RIS)

BibTeX

1088

Views

246

Downloads

Citations

Crossref

N/A

WoS

Scopus

N/A

CSCD

Abstract Full text About this article

Abstract

Biosynthesis of metal nanoparticles presents a promising approach for their efficient and environmentally friendly production. In this study, CuO nanoparticles were successfully synthesized by using Rumex nepalensis Spreng. as a bio-reducing agent. The spectroscopic analysis confirmed the crystalline monoclinic structure of the synthesized CuO NPs, with particle sizes ranging from 21 to 97 nm. These biosynthesized CuO NPs exhibited notable antimicrobial activity against diverse microorganisms, suggesting their potential for antimicrobial applications. Moreover, the CuO NPs displayed significant antioxidant activity, demonstrated by their effective scavenging of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals. This study highlights the straightforward, cost-effective, non-toxic, and robust nature of CuO NPs synthesis using Rumex nepalensis Spreng., offering insights into their potential applications in antimicrobial and antioxidant fields.

Full text

Abstract

Full text

Outline

About this article

Biosynthesis of CuO Nanoparticles Using Plant Extract as a Precursor: Characterization, Antibacterial, and Antioxidant Activity

Show Author's information Hide Author's Information Pawar Abhimanyu^¹(

), Mungole Arvind^², Naktode Kishor^¹

1Department of Chemistry, NevjabaiHitkarini College, Bramhapuri, Maharashtra 441 206, India

2Department of Botany, NevjabaiHitkarini College, Bramhapuri, Maharashtra 441 206, India

Abstract

Keywords: X-ray diffraction (XRD), transmission electron microscopy (TEM), antioxidant activity, antimicrobial activity, CuO nanoparticles (NPs)

References(52)

[1]

I.M. Chung, I. Park, K. Seung-Hyun, et al. Plant-mediated synthesis of silver nanoparticles: Their characteristic properties and therapeutic applications. Nanoscale Research Letters, 2016, 11: 40. https://doi.org/10.1186/s11671-016-1257-4

DOI Google Scholar

[2]

Payal, P. Pandey. A review of recent developments and patents. Recent Patents on Nanotechnology, 2022, 16(1): 45–66.

DOI

[3]

S. Kargozar, M. Mozafari. Nanotechnology and nanomedicine: Start small, think big. Materials Today:Proceedings, 2018, 5(7): 15492−15500. https://doi.org/10.1016/j.matpr.2018.04.155

DOI Google Scholar

[4]

V.K. Yemmireddy, Y.C. Hung. Using photocatalyst metal oxides as antimicrobial surface coatings to ensure food safety—opportunities and challenges. Comprehensive Reviews in Food Science and Food Safety, 2017, 16(4): 617−631. https://doi.org/10.1111/1541-4337.12267

DOI Google Scholar

[5]

M.E. Grigore, E.R. Biscu, A.M. Holban, et al. Methods of synthesis, properties and biomedical applications of CuO nanoparticles. Pharmaceuticals, 2016, 9(4): 75. https://doi.org/10.3390/ph9040075

DOI Google Scholar

[6]

N. Verma, N. Kumar. Synthesis and biomedical applications of copper oxide nanoparticles: An expanding horizon. ACS Biomaterials Science &Engineering, 2019, 5(3): 1170−1188. https://doi.org/10.1021/acsbiomaterials.8b01092

DOI Google Scholar

[7]

N. Silva, S. Ramírez, I. Díaz, et al. Easy, quick, and reproducible sonochemical synthesis of CuO nanoparticles. Materials, 2019, 12(5): 804. https://doi.org/10.3390/ma12050804

DOI Google Scholar

[8]

Y.K. Abdel-Monem, S.M. Emam, H.M.Y. Okda. Solid state thermal decomposition synthesis of CuO nanoparticles from coordinated pyrazolopyridine as novel precursors. Journal of Materials Science:Materials in Electronics, 2017, 28(3): 2923−2934. https://doi.org/10.1007/s10854-016-5877-3

DOI Google Scholar

[9]

M. Chauhan, B. Sharma, R. Kumar, et al. Green synthesis of CuO nanomaterials and their proficient use for organic waste removal and antimicrobial application. Environmental Research, 2019, 168: 85−95. https://doi.org/10.1016/j.envres.2018.09.024

DOI Google Scholar

[10]

K.N. Thakkar, S.S. Mhatre, R.Y. Parikh. Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology. Biology and Medicine, 2010, 6(2): 257−262. https://doi.org/10.1016/j.nano.2009.07.002

DOI Google Scholar

[11]

F.C. Lee, Y.F. Lu, F.C. Chou, et al. Mechanistic study of gas-phase controlled synthesis of copper oxide-based hybrid nanoparticle for CO oxidation. The Journal of Physical Chemistry C, 2016, 120(25): 13638−13648. https://doi.org/10.1021/acs.jpcc.6b05200

DOI Google Scholar

[12]

V.U. Siddiqui, A. Ansari, R. Chauhan, et al. Green synthesis of copper oxide (CuO) nanoparticles by Punica granatum peel extract. Materials Today:Proceedings, 2021, 36: 751−755. https://doi.org/10.1016/j.matpr.2020.05.504

DOI Google Scholar

[13]

A. Azam, A.S. Ahmed, M. Oves, et al. Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and-negative bacterial strains. International Journal of Nanomedicine, 2012, 2012(7): 3527−3535. https://doi.org/10.2147/IJN.S29020

DOI Google Scholar

[14]

A. A. Annu, S. Ahmed. Green Synthesis of Metal, Metal Oxide Nanoparticles, and Their Various Applications. In: Handbook of Ecomaterials. Cham: Springer, 2018: 1–45.

DOI

[15]

S. Ramya, G. Viruthagiri, R. Gobi, et al. Synthesis and characterization of Ni²⁺ ions incorporated CuO nanoparticles and its application in antibacterial activity. Journal of Materials Science:Materials in Electronics, 2016, 27(3): 2701−2711. https://doi.org/10.1007/s10854-015-4080-2

DOI Google Scholar

[16]

Y.Y. Lv, J. Liu, Z.Y. Zhang, et al. Green synthesis of CuO nanoparticles-loaded ZnO nanowires arrays with enhanced photocatalytic activity. Materials Chemistry and Physics, 2021, 267: 124703. https://doi.org/10.1016/j.matchemphys.2021.124703

DOI Google Scholar

[17]

A. Benhammada, D. Trache, S. Chelouche, et al. Catalytic effect of green CuO nanoparticles on the thermal decomposition kinetics of ammonium perchlorate. Zeitschrift Für Anorganische Und Allgemeine Chemie, 2021, 647(4): 312−325. https://doi.org/10.1002/zaac.202000295

DOI Google Scholar

[18]

R.V. Poonguzhali, E.R. Kumar, N. Arunadevi, et al. Natural citric acid assisted synthesis of CuO nanoparticles: Evaluation of structural, optical, morphological properties and colloidal stability for gas sensor applications. Ceramics International, 2022, 48(18): 26287−26293. https://doi.org/10.1016/j.ceramint.2022.05.311

DOI Google Scholar

[19]

K. Dulta, G.K. Ağçeli, P. Chauhan, et al. Multifunctional CuO nanoparticles with enhanced photocatalytic dye degradation and antibacterial activity. Sustainable Environment Research, 2022, 32(1): 2. https://doi.org/10.1186/s42834-021-00111-w

DOI Google Scholar

[20]

M. El Batouti, H.A. Fetouh. A facile new modified method for the preparation of a new cerium-doped lanthanium cuperate perovskite energy storage system using nanotechnology. New Journal of Chemistry, 2021, 45(19): 8506−8515. https://doi.org/10.1039/D1NJ00455G

DOI Google Scholar

[21]

K. Pagar, S.K. Ghotekar, T. Pagar, et al. Antifungal activity of biosynthesized CuO nanoparticles using leaves extract of Moringa oleifera and their structural characterizations. 2020, 3(1): 15–23.

[22]

T. Velusamy, A. Liguori, M. Macias-Montero, et al. Ultra-small CuO nanoparticles with tailored energy-band diagram synthesized by a hybrid plasma-liquid process. Plasma Processes and Polymers, 2017, 14(7): 1600224. https://doi.org/10.1002/ppap.201600224

DOI Google Scholar

[23]

A.A. Mohamed, M. Abu-Elghait, N.E. Ahmed, et al. Eco-friendly mycogenic synthesis of ZnO and CuO nanoparticles for in vitro antibacterial, antibiofilm, and antifungal applications. Biological Trace Element Research, 2021, 199(7): 2788−2799. https://doi.org/10.1007/s12011-020-02369-4

DOI Google Scholar

[24]

V. Balakrishnan, K. Thangaraj, M. Palani, et al. Green synthesis of copper oxide nanoparticles using Euphorbia hirta leaves extract and its biological applications. Inorganic and Nano-Metal Chemistry, 2022, 52(6): 809−818. https://doi.org/10.1080/24701556.2021.1952260

DOI Google Scholar

[25]

A. Waris, M. Din, A. Ali, et al. A comprehensive review of green synthesis of copper oxide nanoparticles and their diverse biomedical applications. Inorganic Chemistry Communications, 2021, 123: 108369. https://doi.org/10.1016/j.inoche.2020.108369

DOI Google Scholar

[26]

H. Al-Jawhari, H. Bin-Thiyab, N. Elbialy, et al. In vitro antioxidant and anticancer activities of cupric oxide nanoparticles synthesized using spinach leaves extract. Nano-Structures & Nano-Objects, 2022, 29: 100815.

DOI

[27]

P. Singh, K.R. Singh, J. Singh, et al. Tunable electrochemistry and efficient antibacterial activity of plant-mediated copper oxide nanoparticles synthesized by Annona squamosa seed extract for agricultural utility. RSC Advances, 2021, 11(29): 18050−18060. https://doi.org/10.1039/D1RA02382A

DOI Google Scholar

[28]

S. Sathiyavimal, S. Vasantharaj, V. Veeramani, et al. Green chemistry route of biosynthesized copper oxide nanoparticles using Psidium guajava leaf extract and their antibacterial activity and effective removal of industrial dyes. Journal of Environmental Chemical Engineering, 2021, 9(2): 105033. https://doi.org/10.1016/j.jece.2021.105033

DOI Google Scholar

[29]

M.W. Shammout, A. Awwad. A novel route for the synthesis of copper oxide nanoparticles using Bougainvillea plant flowers extract and antifungal activity evaluation. Chemistry International, 2021, 7(1): 71−78. https://doi.org/10.5281/zenodo.4042902

DOI Google Scholar

[30]

A. Verma, N. Bharadvaja. Plant-mediated synthesis and characterization of silver and copper oxide nanoparticles: Antibacterial and heavy metal removal activity. Journal of Cluster Science, 2022, 33(4): 1697−1712. https://doi.org/10.1007/s10876-021-02091-8

DOI Google Scholar

[31]

H.M. Chen, X.J. Feng, L. Gao, et al. Inhibiting the PI3K/AKT/mTOR signalling pathway with copper oxide nanoparticles from Houttuynia cordata plant: Attenuating the proliferation of cervical cancer cells. Artificial Cells,Nanomedicine,and Biotechnology, 2021, 49(1): 240−249. https://doi.org/10.1080/21691401.2021.1890101

DOI Google Scholar

[32]

S. Vasantharaj, P. Shivakumar, S. Sathiyavimal, et al. Antibacterial activity and photocatalytic dye degradation of copper oxide nanoparticles (CuONPs) using Justicia gendarussa. Applied Nanoscience, 2023, 13(3): 2295–2302.

DOI

[33]

F. Amin, B. Khattak, A. Alotaibi, et al. Green synthesis of copper oxide nanoparticles using aerva javanica leaf extract and their characterization and investigation of in vitro antimicrobial potential and cytotoxic activities. Evidence-Based Complementary and Alternative Medicine, 2021, 2021: 5589703. https://doi.org/10.1155/2021/5589703

DOI Google Scholar

[34]

M.A. Thakar, S.S. Jha, K. Phasinam, et al. X ray diffraction (XRD) analysis and evaluation of antioxidant activity of copper oxide nanoparticles synthesized from leaf extract of Cissus vitiginea. Materials Today: Proceedings, 2022, 51: 319–324.

DOI

[35]

K. Barman, P. Dutta, D. Chowdhury, et al. Green biosynthesis of copper oxide nanoparticles using waste Colocasia esculenta leaves extract and their application as recyclable catalyst towards the synthesis of 1, 2, 3-triazoles. BioNanoScience, 2021, 11(1): 189−199. https://doi.org/10.1007/s12668-021-00826-5

DOI Google Scholar

[36]

K.S. Shiny, S. Nair, N. Mamatha, et al. Decay resistance of wood treated with copper oxide nanoparticles synthesised using leaf extracts of Lantana camara L. and Nerium oleander L. Wood Material Science &Engineering, 2022, 17(6): 727−733. https://doi.org/10.1080/17480272.2021.1934728

DOI Google Scholar

[37]

J.K. Sharma, P. Srivastava, G. Singh, et al. Catalytic thermal decomposition of ammonium perchlorate and combustion of composite solid propellants over green synthesized CuO nanoparticles. Thermochimica Acta, 2015, 614: 110−115. https://doi.org/10.1016/j.tca.2015.06.023

DOI Google Scholar

[38]

S.A. Akintelu, A.S. Folorunso, F.A. Folorunso, et al. Green synthesis of copper oxide nanoparticles for biomedical application and environmental remediation. Heliyon, 2020, 6(7): e04508. https://doi.org/10.1016/j.heliyon.2020.e04508

DOI Google Scholar

[39]

C. Lamberti, G. Agostini. Characterization of Semiconductor Heterostructures and Nanostructures, 2nd Ed. Elsevier Science: Amsterdam, 2013.

[40]

P. Ananthi, S.M.J. Kala. Plant extract mediated synthesis and characterization of copper nanoparticles and their pharmacological activities. International Journal Innovative Research in Science,Engineering and Technology, 2017, 6(7): 13455−13465.

Google Scholar

[41]

J.A. Wisam, A.J. Haneen. A new paradigm shift to prepare copper nanoparticlesusing biological synthesis and evaluation of antimicrobial activity. Plant Archives, 2018, 18(2): 2020−2024.

Google Scholar

[42]

P. Kuppusamy, S. Ilavenil, S. Srigopalram, et al. Treating of palm oil mill effluent using Commelina nudiflora mediated copper nanoparticles as a novel bio-control agent. Journal of Cleaner Production, 2016, 141: 1023−1029. https://doi.org/10.1016/j.jclepro.2016.09.176

DOI Google Scholar

[43]

M. Maruthupandy, Y. Zuo, J.S. Chen, et al. Synthesis of metal oxide nanoparticles (CuO and ZnO NPs) via biological template and their optical sensor applications. Applied Surface Science, 2017, 397: 167−174. https://doi.org/10.1016/j.apsusc.2016.11.118

DOI Google Scholar

[44]

S.M.H. Akhter, F. Mohammad, S. Ahmad. Terminalia belerica mediated green synthesis of nanoparticles of copper, iron and zinc metal oxides as the alternate antibacterial agents against some common pathogens. BioNanoScience, 2019, 9(2): 365−372. https://doi.org/10.1007/s12668-019-0601-4

DOI Google Scholar

[45]

C. Perez, M. Pauli, P. Bazeuque. An antibiotic assay by agar well diffusion method. Acta Biologiae et Medicinae Experimentalis, 1990, 15: 113−115.

Google Scholar

[46]

M.I. Ahmed, M.S. Hasan, S.J. Uddin, et al. Antinociceptive and antioxidant activities of the ethanolic extract of Excoecaria indica. Dhaka University Journal of Pharmaceutical Sciences, 2007, 6(1): 51−53. https://doi.org/10.3329/dujps.v6i1.344

DOI Google Scholar

[47]

M. Ahamed, H.A. Alhadlaq, M.A.M. Khan, et al. Synthesis, characterization, and antimicrobial activity of copper oxide nanoparticles. Journal of Nanomaterials, 2014, 2014: 637858−434. https://doi.org/10.1155/2014/637858

DOI Google Scholar

[48]

T.B. Vidovix, H.B. Quesada, E.F.D. Januário, et al. Green synthesis of copper oxide nanoparticles using Punica granatum leaf extract applied to the removal of methylene blue. Materials Letters, 2019, 257: 126685. https://doi.org/10.1016/j.matlet.2019.126685

DOI Google Scholar

[49]

O. Antonoglou, K. Lafazanis, S. Mourdikoudis, et al. Biological relevance of CuFeO₂ nanoparticles: Antibacterial and anti-inflammatory activity, genotoxicity, DNA and protein interactions. Materials Science and Engineering:C, 2019, 99: 264−274. https://doi.org/10.1016/j.msec.2019.01.112

DOI Google Scholar

[50]

M. Parthibavarman, V. Sharmila, P. Sathishkumar, et al. A rapid one-pot synthesis of CuO rice-like nanostructure and its structural, optical and electrochemical performance. Journal of Electronic Materials, 2018, 47(9): 5443−5451. https://doi.org/10.1007/s11664-018-6435-y

DOI Google Scholar

[51]

G.T. Anand, S.J. Sundaram, K. Kanimozhi, et al. Microwave assisted green synthesis of CuO nanoparticles for environmental applications. Materials Today:Proceedings, 2021, 36: 427−434. https://doi.org/10.1016/j.matpr.2020.04.881

DOI Google Scholar

[52]

A.K. Mittal, Y. Chisti, U.C. Banerjee. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances, 2013, 31(2): 346−356. https://doi.org/10.1016/j.biotechadv.2013.01.003

DOI Google Scholar

About this article

Publication history

Acknowledgements

Rights and permissions

Publication history

Received: 29 December 2022

Revised: 20 May 2023

Accepted: 20 June 2023

Published: 21 August 2023

Issue date: December 2023

Copyright

Acknowledgements

Acknowledgment

We would like to express our sincere gratitude to the Principal of NevjabaiHitkarini College, Bramhapuri, Maharashtra (India), for their unwavering support, encouragement, and provision of facilities to carry out this research. Additionally, we extend our thanks to Sophisticated Test and Instrumentation Centre (STIC) at Cochin University of Science and Technology, Cochin, Kerala, India, for their assistance in characterizing the samples.

Rights and permissions

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY) (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.