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A novel headspace gas chromatography-mass spectrometry (HS-GC/MS) method for identifying and quantifying 3,3-dimethyl-1-butanol (DMB), a bioactive compound, in extra virgin olive oil was developed. In this study, solvents with similar properties to DΜB but potentially different molecular masses, namely isopropanol, n-butanol, n-pentanol, and 2-pentanol, were tested and 2-pentanol was selected as the internal standard due to both chromatographic separation and distinctive mass spectrum pattern. The method for DMB determination and quantification was validated according to Eurochem CITAC Guide. Sensitivity, specificity, linearity, accuracy, and precision parameters were evaluated. The validation process included an assessment of the method’s robustness and repeatability, ensuring that it produces reliable results for future analyses of olive oil. A linear regression equation was developed for DMB concentrations ranging from 100 to 5,000 µg/L, expressed as y = x.11.462.10−6, where y represents the peak area ratio and x is the DMB concentration in µg/L. This equation has a high correlation coefficient of 0.9989 (R2), and the uncertainty budget was estimated to be 8.22% at a confidence level of k = 2. In addition, the lower concentration range of 0 to 100 µg/L was characterized by the equation y = x.17.860.10−6 which has a correlation coefficient of 0.9983 (R2). The uncertainty of DMB measurements in extra virgin olive oil was estimated to be 7.0% at a confidence level of k = 2. This new method was applied to determine the presence of DMB in various olive oil samples from Greece and Türkiye. The DMB values for olive oil samples were: N1(Plus health blue multi varietal) 9.7 μg/L, Ν2 (Plus health DMB multi varietal) 11.4 μg/L, N3 (Fyllikon first harvest organic) 8.3 μg/L, N4 (Plus health green multi varietal) 7.8 μg/L, N5 (Agourelaio early harvest organic) 6.8 μg/L, N6 (unknown) 4.8 μg/L, N7 (Armonia monovarietal organic) 4.8 μg/L and N8 (Edremit type olive oil from Kırkağaç, Manisa) 8.8 μg/L and N9 (Market product in Türkiye) 1.5 μg/L.
Casso, A.G., VanDongen, N.S., Gioscia-Ryan, R.A., Clayton, Z.S., Greenberg, N.T., Ziemba, B.P., Hutton, D.A., Neilson, A.P., Davy, K.P., Seals, D.R., and Brunt, V.E. (2022). Initiation of 3,3-dimethyl-1-butanol at midlife prevents endothelial dysfunction and attenuates in vivo aortic stiffening with ageing in mice. J. Physiol 600(21): 4633–4651.
García-Alegría, A.M., Anduro-Corona, I., Pérez-Martínez, C.J., Quizán-Plata, T., Villegas, L.A., Rascón-Durán, M.L., and Astiazaran-Garcia, H. (2023). DNA quantification via nanodrop spectrophotometer: estimation of method uncertainty using DNA from standard reference materials, sprague dawley rats, and humans. J. Chem. Metrol. 17(1): 25–41.
Hsu, C.N., Hou, C.Y., Lee, C.T., Chang-Chien, G.P., Lin, S., and Tain, Y.L. (2021). Maternal 3,3-Dimethyl-1-Butanol Therapy Protects Adult Male Rat Offspring against Hypertension Programmed by Perinatal TCDD Exposure. Nutrients 13(9): 3041.
Huang, Y., Lin, F., Tang, R., Bao, C., Zhou, Q., Ye, K., Shen, Y., Liu, C., Hong, C., Yang, K., Tang, H., Wang, J., Lu, W., and Wang, T. (2022). Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary Hypertension. Am. J. Respir. Cell Mol. Biol. 66(4): 452–460.
Kiritsakis, A.K., and Karamesinis-Kiritsakis, E. (2017). The miracle of olives and olive oil. CCity Publish Co, Thessaloniki Greece.
Kiritsakis, A., and Keçeli Mutlu, T. (2023). Extra Virgin Olive Oil Composition and its Bioactive Phenolic Compounds. Novel Tech. Nutr. Food Sci. 7(1): 654–657.
Kiritsakis, A.K., Kiritsakis, K.A., and Tsitsipas, C.K. (2020b). A review of the evolution in the research of antioxidants in olives and olive oil during the last four decades. J. Food Bioact. 11: 31–56.
Magnusson, B., and Omemark, U., editors. (2014). Eurachem guide: the fitness for purpose of analytical methods: a laboratory guide to method validation and related topics. 2nd ed. Eurachem/CITAC, Teddington, UK.
Manach, C., Scalbert, A., Morand, C., Rémésy, C., and Jiménez, L. (2004). Polyphenols: food sources and bioavailability. Am. J. Clin. Nutr. 79: 727–747.
Mao, J., Zhao, P., Wang, Q., Chen, A., Li, X., Li, X., Liu, T., Tao, Z., Wang, X., Du, Y., Gong, M., Song, L., Gao, Y., and Shi, H. (2021). Repeated 3,3-Dimethyl-1-butanol exposure alters social dominance in adult mice. Neurosci. Lett. 758: 136006.
Micheli, L., Bertini, L., Bonato, A., Villanova, N., Caruso, C., Caruso, M., Bernini, R., and Tirone, F. (2023). Role of Hydroxytyrosol and Oleuropein in the Prevention of Aging and Related Disorders: Focus on Neurodegeneration, Skeletal Muscle Dysfunction and Gut Microbiota. Nutrients 15(7): 1767.
Ozbay, T., Gurbetoglu, G.P., Sarracoglu, N., Aybey-Doganay, A., and Pinarbasli, O. (2023). A validated RP-HPLC assay method for Tofacitinib in pharmaceutical drug products. J. Chem. Metrol. 17: 139–147.
Ozer, Z., Carikci, S., Kilic, T., Selvi, S., and Goren, A.C. (2024). Determination of the effect of different drying methods on secondary metabolites of Lavandula pedunculata (Mill.) Cav. subsp. cariensis (Boiss.) Upson & S. Andrews by LC-HRMS. J. Chem. Metrol. 18(2): 124–133.
Shah, K., Chokshi, A., and Vyas, N. (2024). Development of RP-HPLC-DAD method for quantitative analysis of quercetin and piperine in botanical extracts. J. Chem. Metrol. 18(2): 114–123.
Wang, G., Kong, B., Shuai, W., Fu, H., Jiang, X., and Huang, H. (2020). 3,3-Dimethyl-1-butanol attenuates cardiac remodeling in pressure-overload-induced heart failure mice. J. Nutr. Biochem. 78: 108341.
Wang, Z., Roberts, A.B., Buffa, J.A., Didonato, J.A., Lusis, A.J., and Hazen, S.L. (2015). Non-lethal inhibition of Gut Microbial Trimethylamine Production for the Treatment of Atherosclerosis. Cell 163(7): 1585–1895.
Yang, Y., Zeng, Q., Gao, J., Yang, B., Zhou, J., Li, K., Li, L., Wang, A., Li, X., Liu, Z., Luo, Q., Zhao, Z., Liu, B., Xue, J., Jiang, X., Konerman, M.C., Zheng, L., and Xiong, C. (2022). High-circulating gut microbiota-dependent metabolite trimethylamine N-oxide is associated with poor prognosis in pulmonary arterial hypertension. Eur. Heart J. Open 2(5): oeac021.
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