Effects of Opuntia ficus-indica fruit juice-fermented by Lactiplantibacillus plantarum HNU082 on kidney injury, gut microbiota and metabolites in hyperuricemia mice based on multi-omics techniques

Hyperuricemia (HUA), as a disease of purine metabolic disorder, seriously affects human health, mainly manifested in kidney injury, gut microbiota dysbiosis, and metabolic disorders. In this study, C57BL/6J mice were used to establish a HUA model by a combination of potassium oxonate and adenine to investigate the effect of fermented Opuntia ficus-indica juice-based Lactiplantibacillus plantarum HNU082 (OFIBP) on HUA. The results showed that OFIBP alleviated kidney inflammation and gut damage in HUA mice and regulate uric acid (UA) homeostasis in HUA mice through modulating UA production and excretion, which was evidenced by enhancing the expression of UA excretory protein and gene and decreasing the activity of key enzymes of UA synthesis. In addition, OFIBP supplementation restored HUA-induced gut microbiota disturbance, as well as increased metabolites of short-chain fatty acids (SCFAs) levels. Importantly, OFIBP supplementation modulated intestinal metabolic dysbiosis and mediated amino acid metabolism to ameliorate HUA in mice. Therefore, probiotic fermented juice could play a positive role in improving HUA as a novel and efficient functional food.