Toxic-oriented Biomimetic Nanoplatform for Screening Food Sample Unknown Risks

Screening of unknown risks is a challenge for food regulation. However, there are still some limitations in screening unknown risk compounds with traditional methods. In this study, a toxic-oriented biomimetic platform, high-expression epidermal growth factor receptor cell membranes coated magnetic nanoparticles (EGFR/MCMNs), was innovatively applied to screen potential risk substances from food samples. EGFR/MCMNs were constructed by covalently immobilizing cell membranes on the magnetic nanoparticles (MNPs). The resultant EGFR/MCMNs exhibited satisfactory bioactivity, enough magnetic properties and excellent adsorption capacity, enabling accurate targeting of the possible substances. Ultimately, two potential risk compounds, BPA and BPAF, were successfully captured from the canned food of long-term placement. Their preliminary toxicology was further validated that the screened compounds could activate EGFR downstream signaling pathways and lead to cellular oxidative damage. This proposed method highlights the potential of toxic-oriented platforms for screening risk substances from complicated food matrices.