A Continuous Flow Non-Thermal Antibacterial Platform for Foodborne Pathogens Based on Halbach Ring Mediated Magnetic Net

In food processing, avoiding contamination of pathogens while not affecting the nutritional composition of foods is crucial. Herein, a Halbach ring mediated continuous flow non-thermal antibacterial (HCFNA) platform for the eradication of pathogenic bacteria is developed by employing magnetic nanoparticle@Ag Prussian blue analogues (MNP@AgPBAs). MNP@AgPBAs with excellent non-thermal antibacterial property and magnetic responsive performance are synthesized through in-situ growth method and ion exchange process. Particularly, the Halbach ring can enrich and align magnetic materials to form a magnetic net, enhancing the contact between materials and bacteria. Moreover, the Halbach ring can be integrated with a microinjection pump to achieve continuous flow antibacterial operation. The safety of MNP@AgPBAs is confirmed by in vitro cytotoxicity tests, indicating the admirable biocompatibility of MNP@AgPBAs and their promising application in the food industry. The HCFNA platform acquires sterilization efficiencies of 99.91% and 98.19% against Gram-negative bacteria （Escherichia coli, E. coli) and Gram-positive bacteria （Staphylococcus aureus, S. aureus), respectively. Besides, the HCFNA platform exhibits robust antibacterial activity in real food samples such as milk, tea, and drinking water, showcasing well-adapted properties to diverse liquid food matrices. Compared to heat treatment, the HCFNA platform exerts a lesser impact on actual samples, which have been proven by nutrient quality analysis. Hence, the HCFNA platform possesses the capability to realize continuous flow antibacterial operation and reduce nutrient loss in food production and processing, making it a promising candidate for antibacterial platforms in the food industry.