Characterization of polysaccharides isolated from Dendrobium loddigesii Rolfe and study of its hepatoprotective activity in vivo

The polysaccharides extracted from Dendrobium loddigesii (DLPs) possess significant nutritional and functional properties that position them as promising candidates for the development of functional foods. The rising incidence of liver diseases, particularly those induced by drugs and environmental toxins, underscores the urgent need for safer, more effective therapeutic interventions. Liver injury, characterized by its complex pathophysiology, can lead to severe outcomes, including acute liver failure, highlighting the limitations of traditional therapies, which often entail adverse effects. This study explores the potential of DLPs as hepatoprotective agents against carbon tetrachloride (CCL₄)-induced liver injury in murine models, with a focus on their health-promoting effects. DLPs were extracted and structurally characterized, followed by in vivo assessments of their efficacy in mitigating liver damage. Results demonstrated that DLPs reduced serum markers of liver injury (ALT, AST, AKP) and alleviated hepatocyte necrosis and inflammation. Furthermore, metabolomic analysis revealed that DLPs modulate lipid metabolism and the gut-liver axis, suggesting their role in reducing oxidative stress and inflammation. Notably, DLPs exhibited prebiotic-like effects by enhancing gut microbiota diversity, further supporting their potential as functional food ingredients. These findings provide a strong basis for the nutritional and health benefits of DLPs, highlighting their application in promoting liver health and overall wellness within the context of functional foods.