Polyphenol-rich extract from Sanghuangporous vaninii against acetaminophen-induced liver injury by blocking ferroptosis via NRF2/GPX4 pathway

Acetaminophen (APAP) used as an antipyretic and analgesic agent can cause acute liver injury (AILI) under overdose. Sanghuangporous vaninii is an edible fungus with abundant metabolites exhibits excellent hepatoprotective activities, but the effect for AILI is not yet fully understood. In this study, the polyphenol-rich extract from S. vaninii (PSV) was prepared, with a total phenolic content of 75.72% and 34 compounds. The data of hepatoprotection indicated that PSV obviously alleviated the hepatocellular injury induced by APAP in vivo and in vitro. The protective mechanism of PSV against APAP-induced AILI might be attributed to the activating NRF2/GPX4 pathway. Based on network pharmacology analysis, the active components of PSV such as caffeic acid, osmundacetone and hispolone played a key role in hepatoprotection of PSV. Consequently, this study highlights the protection of PSV on AILI, which provides new insight into bioactivities of S. vaninii.