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Open Access

Application of solid-phase extraction and gas chromatography-mass spectrometry (SPE-GC-MS) in resolution of metabolism pattern of higher alcohols in rat plasma

Yufei Liua,b, Xiaonian Caoa,b,c, Zhilei Zhoua,d, Qingxi Rena,d, Zhongwei Jia,d, Min Gonge, Yuezheng Xue, Weibiao Zhoud,f, Shuguang Cheng( ), Jian Maoa,b,d,e( )
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Wuxi 214122, China
Luzhou Laojiao Co., Ltd., Luzhou 646000, China
Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health, Jiangnan University (Shaoxing) Industrial Technology Research Institute, Shaoxing 312000, China
National Engineering Research Center for Huangjiu, Zhejiang Guyuelongshan Shaoxing Wine Co., Ltd., Zhejiang Shaoxing Huangjiu Industry Innovation Service Complex, Shaoxing 312000, China
Department of Food Science and Technology, National University of Singapore, Singapore 117542, Singapore
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC), Beijing 100730, China

Peer review under responsibility of Tsinghua University Press.

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Highlights

• An effective SPE-GC/MS strategy to quantify plasma higher alcohols.

• Blood tracking of eight higher alcohols after feeding huangjiu, wine, baijiu and brandy.

• The fastest metabolism of plasma total higher alcohols was observed in huangjiu group.

• Metabolism of plasma higher alcohols may differ at the same gavage dosage.

Graphical Abstract

Abstract

Higher alcohols are key factors affecting sensory quality and post-drinking comfort of alcoholic beverages. A strategy combining solid-phase extraction and gas chromatography-mass spectrometry (SPE-GC-MS) was established to analyze the metabolism pattern of higher alcohols in rat plasma after gavage of 4 common alcoholic beverages including huangjiu, baijiu, wine and brandy.7 mL of dichloromethane was determined as the optimal extraction condition, and 8 higher alcohols were precisely quantified with detection limits of 1.82−11.65 μg/L, recoveries of 89.07%−110.89% and fine repeatability. The fastest absorption and elimination rates of plasma total higher alcohols were observed in baijiu and huangjiu group, respectively, and the highest peak concentration was found in brandy group. Additionally, the metabolic rate of plasma isoamyl alcohol in huangjiu group was faster than that in wine group at the same intragastric administration dosage. This study may provide potential insight for evaluation of alcoholic beverage quality.

Keywords

Higher alcoholsAlcoholic beveragesSPE-GC-MSPharmacokinetic parameters

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Food Science and Human Wellness
Volume 13 Issue 6,
November 2024
Pages 3501-3510
DOI: 10.26599/FSHW.2023.9250033
Cite this article:
Liu Y, Cao X, Zhou Z, et al. Application of solid-phase extraction and gas chromatography-mass spectrometry (SPE-GC-MS) in resolution of metabolism pattern of higher alcohols in rat plasma. Food Science and Human Wellness, 2024, 13(6): 3501-3510. https://doi.org/10.26599/FSHW.2023.9250033

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Received: 25 January 2023
Revised: 06 March 2023
Accepted: 27 March 2023
Published: 18 December 2024
© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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