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Food Science and Human Wellness 2024, 13(3): 1628-1635 https://doi.org/10.26599/FSHW.2022.9250138
Research Article | Open Access | Issue | Published: 08 February 2024

Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells

Show Author's Information Zhuqing Daia,1,,  Meimei Niea,1,  Ye Chena,  Jiangfeng Songa,  Yayuan Xua,  Zhongyuan Zhanga,b( ),  Guodong Zhangc,  Shumo Yand,  Xing Zhangc,  Dajing Lia( )
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
Jiangsu Aland Nutrition Co., Ltd., Taizhou 214500, China
Aland Nutrition Taizhou Co., Ltd., Taizhou 225300, China

1 These authors contributed equally to this study.

Peer review under responsibility of Tsinghua University Press.

Keywords:
Mechanism, Antioxidant, Lutein, Stevioside, Human retinal pigment epithelial cell
Cite this article:
Dai Z, Nie M, Chen Y, et al. Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells. Food Science and Human Wellness, 2024, 13(3): 1628-1635. https://doi.org/10.26599/FSHW.2022.9250138
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Abstract Full text About this article

In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT) and (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.


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About this article

Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells

Show Author's information Zhuqing Daia,1,, Meimei Niea,1, Ye Chena, Jiangfeng Songa, Yayuan Xua, Zhongyuan Zhanga,b( ), Guodong Zhangc, Shumo Yand, Xing Zhangc, Dajing Lia( )
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
Jiangsu Aland Nutrition Co., Ltd., Taizhou 214500, China
Aland Nutrition Taizhou Co., Ltd., Taizhou 225300, China

1 These authors contributed equally to this study.

Peer review under responsibility of Tsinghua University Press.

Abstract

In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT) and (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.

Keywords: Mechanism, Antioxidant, Lutein, Stevioside, Human retinal pigment epithelial cell

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Received: 16 November 2022
Revised: 07 December 2022
Accepted: 19 December 2022
Published: 08 February 2024
Issue date: May 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

Acknowledgement

The present work received research funding from the National Natural Science Foundation of China (31801541), the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province (CX(22)3065), Major Scientific and Technological Achievements Transformation Project of Taizhou (SCG 202105), and the Taizhou Science and Technology Support Plan (TN202106).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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