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Food Science and Human Wellness 2024, 13(3): 1494-1502 https://doi.org/10.26599/FSHW.2022.9250126
Research Article | Open Access | Issue | Published: 08 February 2024

Lactose-free milk powder can effectively relieve diarrhea symptoms in weaning SD rats and children

Show Author's Information Manman Liua,  Shiwen Hana,  Boya Lia,  Cheng Chena,  Lu Yaoa,  Jung-il Kwonb,  Juan Jinb,  Huilian Chea,( )
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Maeil Innovation Center, Maeil Dairies Co., Ltd., Seoul 03142, South Korea

Peer review under responsibility of Tsinghua University Press.

Keywords:
Gut microbiota, Intestinal barrier, Diarrhea, Lactose-free, Milk powder
Cite this article:
Liu M, Han S, Li B, et al. Lactose-free milk powder can effectively relieve diarrhea symptoms in weaning SD rats and children. Food Science and Human Wellness, 2024, 13(3): 1494-1502. https://doi.org/10.26599/FSHW.2022.9250126
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Abstract Full text About this article

Diarrhea has become the leading cause of illness and death among infants and young children in developing countries. Clinically, patients with diarrhea showed damaged intestinal epithelial villi, usually accompanied by lactase def iciency. In this study, we evaluated the therapeutic effects of lactose-free milk powder on rats and children with diarrhea. Antibiotic-associated diarrhea (AAD) model was established by gavage with antibiotic mixture in SD rats, followed by administration of milk powder containing lactose or not. The results showed that lactose-free milk powder ameliorated AAD-related diarrhea symptoms, and accelerated the recovery from diarrhea. And 16S sequencing results indicated lactose-free milk powder contributed to increase the α- and β-diversity of intestinal f lora, and restore the intestinal microbiota disorder. In conclusion, our data demonstrate that lactose-free milk powder could alleviate diarrhea by restoring gut microbiota and intestinal barrier function.


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About this article

Lactose-free milk powder can effectively relieve diarrhea symptoms in weaning SD rats and children

Show Author's information Manman Liua, Shiwen Hana, Boya Lia, Cheng Chena, Lu Yaoa, Jung-il Kwonb, Juan Jinb, Huilian Chea,( )
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Maeil Innovation Center, Maeil Dairies Co., Ltd., Seoul 03142, South Korea

Peer review under responsibility of Tsinghua University Press.

Highlights

• Lactose-free milk powder could alleviate diarrhea symptoms but not affect normal growth in both antibiotic-associated diarrhea rats and children.

• Lactose-free milk powder promoted the recovery of gut barrier integrity.

• Lactose-free milk powder played a vital role in regulating gut microbial structure and diversity.

• Lactose-free milk powder enhanced the production of short chain fatty acids.

Abstract

Diarrhea has become the leading cause of illness and death among infants and young children in developing countries. Clinically, patients with diarrhea showed damaged intestinal epithelial villi, usually accompanied by lactase def iciency. In this study, we evaluated the therapeutic effects of lactose-free milk powder on rats and children with diarrhea. Antibiotic-associated diarrhea (AAD) model was established by gavage with antibiotic mixture in SD rats, followed by administration of milk powder containing lactose or not. The results showed that lactose-free milk powder ameliorated AAD-related diarrhea symptoms, and accelerated the recovery from diarrhea. And 16S sequencing results indicated lactose-free milk powder contributed to increase the α- and β-diversity of intestinal f lora, and restore the intestinal microbiota disorder. In conclusion, our data demonstrate that lactose-free milk powder could alleviate diarrhea by restoring gut microbiota and intestinal barrier function.

Keywords: Gut microbiota, Intestinal barrier, Diarrhea, Lactose-free, Milk powder

References(39)

[1]

L. Liu, H.L. Johnson, S. Cousens, Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000, Lancet 380 (2012) 1308-1308. https://doi.org/10.1016/S0140-6736(12)60560-1.
DOI Google Scholar
[2]

R. Black, O. Fontaine, L. Lamberti, et al., Drivers of the reduction in childhood diarrhea mortality 1980–2015 and interventions to eliminate preventable diarrhea deaths by 2030, J. Glob. Health 9 (2019) 020801. https://doi.org/10.7189/jogh.09.020801.
DOI Google Scholar
[3]

O. Gomez-Escudero, J.M. Remes-Troche, Approach to the adult patient with chronic diarrhea: a literature review. Rev. Gastroenterol Mex. (Engl Ed) 86 (2021) 387-402. https://doi.org/10.1016/j.rgmxen.2021.08.007.
DOI Google Scholar
[4]

B.E. Lacy, F. Mearin, L. Chang, et al., Bowel disorders, Gastroenterology 150 (2016) 1393-1407. https://doi.org/10.1053/j.gastro.2016.02.031.
DOI Google Scholar
[5]

M. Pimentel, Evidence-based management of irritable bowel syndrome with diarrhea, Am. J. Manag. Care 24 (2018) S35-S46.
Google Scholar
[6]

C. Akoua-Koffi, V. Akran, I. Peenze, et al., Epidemiological and virological aspects rotavirus diarrhoea in Abidjan, Cote d’Ivoire (1997–2000), Bull. Soc. Pathol. Exot. 100 (2007) 246-249.
Google Scholar
[7]

B. Clark, M. McKendrick, A review of viral gastroenteritis, Curr. Opin. Infect. Dis. 17 (2004) 461-469. https://doi.org/10.1097/00001432-200410000-00011.
DOI Google Scholar
[8]

H. Wang, C.A. Liddell, M.M. Coates, et al., Global, regional, and national levels of neonatal, infant, and under-5 mortality during 1990–2013: a systematic analysis for the Global Burden of Disease Study 2013, Lancet 384 (2014) 957-979. https://doi.org/10.1016/S0140-6736(14)60497-9.
DOI Google Scholar
[9]

A. Clark, R. Black, J. Tate, et al., Estimating global, regional and national rotavirus deaths in children aged＜5 years: current approaches, new analyses and proposed improvements, PLoS ONE 12 (2017) e0183392. https://doi.org/10.1371/journal.pone.0183392.
DOI Google Scholar
[10]

J.E. Bines, N.T. Liem, F.A. Justice, et al., Risk factors for intussusception in infants in Vietnam and Australia: adenovirus implicated, but not rotavirus, J. Pediatr. 149 (2006) 452-460. https://doi.org/10.1016/j.jpeds.2006.04.010.
DOI Google Scholar
[11]

Y. Hoshino, A.Z. Kapikian, Rotavirus serotypes: classification and importance in epidemiology, immunity, and vaccine development, J. Health Popul. Nutr. 18 (2000) 5-14.
Google Scholar
[12]

T. Niewold, M.M. Hulst, H.H.D. Kerstens, et al., The in vivo early transcriptional intestinal response to rotavirus infection in Germ-free piglets, Vet. Immunol. Immunop. 128 (2009) 262-263. https://doi.org/10.1016/j.vetimm.2008.10.113.
DOI Google Scholar
[13]

C.A. Omatola, A.O. Olaniran, Rotaviruses: from pathogenesis to disease control-a critical review, Viruses 14 (2022) 875. https://doi.org/10.3390/v14050875.
DOI Google Scholar
[14]

R. Bishop, G.P. Davidson, I.H. Holmes, et al., Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis, Lancet 302 (1973) 1281-1283. https://doi.org/10.1016/s0140-6736(73)92867-5.
DOI Google Scholar
[15]

J. Gomez-Rial, S. Sanchez-Batan, I. Rivero-Calle, et al., Rotavirus infection beyond the gut, Infect. Drug Resist. 12 (2019) 55-64. https://doi.org/10.2147/IDR.S186404.
DOI Google Scholar
[16]

V. Gonzalez-Galan, A. Sanchez-Fauqier, I. Obando, et al., High prevalence of community-acquired norovirus gastroenteritis among hospitalized children: a prospective study, Clin. Microbiol. Infec. 17 (2011) 1895-1899. https://doi.org/10.1111/j.1469-0691.2011.03506.x.
DOI Google Scholar
[17]

M. Pimentel, H.C. Lin, P. Enayati, et al., Methane, a gas produced by enteric bacteria, slows intestinal transit and augments small intestinal contractile activity, Am. J. Physiol-Gastr. L. 290 (2006) G1089-G1095. https://doi.org/10.1152/ajpgi.00574.2004.
DOI Google Scholar
[18]

S.B. Matthews, J.P. Waud, A.G. Roberts, et al., Systemic lactose intolerance: a new perspective on an old problem, Postgrad. Med. J. 81 (2005) 167-173. https://doi.org/10.1136/pgmj.2004.025551.
DOI Google Scholar
[19]

S.C. Arya, Rotaviral infection and intestinal lactase level, J. Infect. Dis. 150 (1984) 791-791. https://doi.org/10.1093/infdis/150.5.791.
DOI Google Scholar
[20]

Q.W. Meng, S.S. Sun, Z. Luo, et al., Maternal dietary resveratrol alleviates weaning-associated diarrhea and intestinal inflammation in pig offspring by changing intestinal gene expression and microbiota, Food Funct. 10 (2019) 5626-5643. https://doi.org/10.1039/c9fo00637k.
DOI Google Scholar
[21]

B.L. Zhou, Y.T. Yuan, S.S. Zhang, et al., Intestinal flora and disease mutually shape the regional immune system in the intestinal tract, Front. Immunol. 11 (2020) 575. https://doi.org/10.3389/fimmu.2020.00575
DOI Google Scholar
[22]

R.V. Luthold, G.R. Fernandes, A.C. Franco de Moraes, et al., Gut microbiota interactions with the immunomodulatory role of vitamin D in normal individuals, Metabolism 69 (2017) 76-86. https://doi.org/10.1016/j.metabol.2017.01.007.
DOI Google Scholar
[23]

W. Zhang, B. Zhu, J. Xu, et al., Bacteroides fragilis protects against antibiotic-associated diarrhea in rats by modulating intestinal defenses, Front. Immunol. 9 (2018) 1040. https://doi.org/10.3389/fimmu.2018.01040.
DOI Google Scholar
[24]

M.N. Hung, Z. Xia, N.T. Hu, et al., Molecular and biochemical analysis of two β-galactosidases from Bifidobacterium infantis HL96, Appl. Environ. Microbiol. 67 (2001) 4256-4263. https://doi.org/10.1128/AEM.67.9.4256-4263.2001.
DOI Google Scholar
[25]

I. Darah, W.Y. Tong, S. Nor-Afifah, et al., Antimicrobial effects of Caulerpa sertularioides extract on foodborne diarrhea-caused bacteria, Eur. Rev. Med. Pharmacol. Sci. 18 (2014) 171-178.
Google Scholar
[26]

C. Chen, Q. Yin, H. Wu, et al., Different effects of premature infant formula and breast milk on intestinal microecological development in premature infants, Front. Microbiol. 10 (2019) 3020. https://doi.org/10.3389/fmicb.2019.03020.
DOI Google Scholar
[27]
WHO Division of Diarrhoeal and Acute Respiratory Disease Control. Technical Advisory Group, Meeting (1995: Geneva, Switzerland) & World Health Organization. Division of Diarrhoeal and Acute Respiratory Disease Control. (1995). Division of Diarrhoeal and Acute Respiratory Disease Control : report of the meeting of the Technical Advisory Group, Geneva, 3–7 April 1995. https://apps.who.int/iris/handle/10665/58325.
[28]

C. Li, G. Pi, F. Li, The role of intestinal flora in the regulation of bone homeostasis, Front. Cell Infect. Microbiol. 11 (2021) 579323. https://doi.org/10.3389/fcimb.2021.579323.
DOI Google Scholar
[29]

G. Zhu, F. Ma, G. Wang, et al., Bifidobacteria attenuate the development of metabolic disorders, with inter- and intra-species differences, Food Funct. 9 (2018) 3509-3522. https://doi.org/10.1039/c8fo00100f.
DOI Google Scholar
[30]

B. Alemayehu, B.T. Ayele, C. Valsangiacomo, et al., Spatiotemporal and hotspot detection of U5-children diarrhea in resource-limited areas of Ethiopia, Sci. Rep. 10 (2020) 10997. https://doi.org/10.1038/s41598-020-67623-0.
DOI Google Scholar
[31]

D. Huang, Y. Chen, H. Chen, et al., Supplementation of Bacillus sp. DU-106 alleviates antibiotic-associated diarrhea in association with the regulation of intestinal microbiota in mice, Probiotics Antimicro. 14 (2022) 372-383. https://doi.org/10.1007/s12602-022-09906-8.
DOI Google Scholar
[32]

J.H. Xu, Y. Huang, Efficiency of lactose-free formula feeding as an adjunctive therapy in infants with acute diarrhea, Chinese Journal of Contemporary Pediatrics 11 (2009) 532-536.
Google Scholar
[33]

N. Simakachorn, Y. Tongpenyai, O. Tongtan, et al., Randomized, double-blind clinical trial of a lactose-free and a lactose-containing formula in dietary management of acute childhood diarrhea, J. Med. Assoc. Thai. 87 (2004) 641-649.
DOI Google Scholar
[34]

M.J. Nasiri, M. Goudarzi, B. Hajikhani, et al., Clostridioides (Clostridium)difficile infection in hospitalized patients with antibiotic-associated diarrhea:a systematic review and meta-analysis, Anaerobe 50 (2018) 32-37. https://doi.org/10.1016/j.anaerobe.2018.01.011.
DOI Google Scholar
[35]

B.P. Willing, S.L. Russell, B.B. Finlay, Shifting the balance: antibiotic effects on host-microbiota mutualism, Nat. Rev. Microbiol. 9 (2011) 233-243. https://doi.org/10.1038/nrmicro2536.
DOI Google Scholar
[36]

B. Yang, Z. Huang, Z.J. He, et al., Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli, Food Funct. 12 (2021) 7271-7282. https://doi.org/10.1039/d1fo00504a.
DOI Google Scholar
[37]

S.C. Kyriakis, V.K. Tsiloyiannis, J. Vlemmas, et al. The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets, Res. Vet. Sci. 67 (1999) 223-228. https://doi.org/10.1053/rvsc.1999.0308.
DOI Google Scholar
[38]

J. Cao, Z.M. Yu, W.Y. Liu, et al., Probiotic characteristics of Bacillus coagulans and associated implications for human health and diseases, J. Funct. Foods 64 (2020). https://doi.org/10.1016/j.jff.2019.103643.
DOI Google Scholar
[39]

A. Koh, F. de Vadder, P. Kovatcheva-Datchary, et al., From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites, Cell 165 (2016) 1332-1345. DOI:10.1016/j.cell.2016.05.041.
DOI Google Scholar
Publication history
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Publication history

Received: 10 October 2022
Revised: 28 October 2022
Accepted: 02 December 2022
Published: 08 February 2024
Issue date: May 2024

Copyright

© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

This work was supported by the project of two children’s dairy products (201704810610483).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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