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Food Science and Human Wellness 2024, 13(3): 1201-1211 https://doi.org/10.26599/FSHW.2022.9250100
Research Article | Open Access | Issue | Published: 08 February 2024

Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation

Show Author's Information Dingwu Qua,b,,  Zhennan Gua,b,  Saisai Fenga,b,  Leilei Yua,b,  Fengwei Tiana,b,  Hao Zhanga,b,c,d,  Wei Chena,b,c,  Qixiao Zhaia,b( )
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China

Peer review under responsibility of Tsinghua University Press.

Keywords:
Intestinal barrier, Gut commensals, Immunoregulation, Dextran sulfate sodium (DSS) colitis
Cite this article:
Qu D, Gu Z, Feng S, et al. Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation. Food Science and Human Wellness, 2024, 13(3): 1201-1211. https://doi.org/10.26599/FSHW.2022.9250100
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Abstract Full text Electronic supplementary material About this article

Inflammatory bowel disease (IBD) is a complex relapsing inflammatory disease in the gut and is driven by complicated host-gut microbiome interactions. Gut commensals have shown different functions in IBD prevention and treatment. To gain a mechanistic understanding of how different commensals affect intestinal inflammation, we compared the protective effects of 6 probiotics (belonging to the genera Akkermansia, Bifidobacterium, Clostridium, and Enterococcus) on dextran sulfate sodium (DSS)-induced colitis in mice with or without gut microbiota. Anti-inflammatory properties (ratio of interleukin (IL)-10 and IL-12) of these strains were also evaluated in an in vitro mesenteric lymph nodes (MLN) co-culture system. Results showed that 4 probiotics (belonging to the species Bifidobacterium breve, Bifidobacterium bifidum, and Enterococcus faecalis) can alleviate colitis in normal mice. The probiotic strains differed in regulating the intestinal microbiota, cytokines (IL-10, IL-1β and interferon (IFN)-γ), and tight junction function (Zonulin-1 and Occludin). By constrast, Akkermansia muciniphila AH39 and Clostridium butyricum FHuNHHMY49T1 were not protective. Interestingly, B. breve JSNJJNM2 with high anti-inflammatory potential in the MLN model could relieve colitis symptoms in antibiotic cocktail (Abx)-treated mice. Meanwhile, E. faecalis FJSWX25M1 induced low levels of cytokines in vitro and showed no beneficial effects. Therefore, we provided insight into the clinical application of probiotics in IBD treatment.


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Electronic supplementary material
About this article

Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation

Show Author's information Dingwu Qua,b,, Zhennan Gua,b, Saisai Fenga,b, Leilei Yua,b, Fengwei Tiana,b, Hao Zhanga,b,c,d, Wei Chena,b,c, Qixiao Zhaia,b( )
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi 214122, China

Peer review under responsibility of Tsinghua University Press.

Abstract

Inflammatory bowel disease (IBD) is a complex relapsing inflammatory disease in the gut and is driven by complicated host-gut microbiome interactions. Gut commensals have shown different functions in IBD prevention and treatment. To gain a mechanistic understanding of how different commensals affect intestinal inflammation, we compared the protective effects of 6 probiotics (belonging to the genera Akkermansia, Bifidobacterium, Clostridium, and Enterococcus) on dextran sulfate sodium (DSS)-induced colitis in mice with or without gut microbiota. Anti-inflammatory properties (ratio of interleukin (IL)-10 and IL-12) of these strains were also evaluated in an in vitro mesenteric lymph nodes (MLN) co-culture system. Results showed that 4 probiotics (belonging to the species Bifidobacterium breve, Bifidobacterium bifidum, and Enterococcus faecalis) can alleviate colitis in normal mice. The probiotic strains differed in regulating the intestinal microbiota, cytokines (IL-10, IL-1β and interferon (IFN)-γ), and tight junction function (Zonulin-1 and Occludin). By constrast, Akkermansia muciniphila AH39 and Clostridium butyricum FHuNHHMY49T1 were not protective. Interestingly, B. breve JSNJJNM2 with high anti-inflammatory potential in the MLN model could relieve colitis symptoms in antibiotic cocktail (Abx)-treated mice. Meanwhile, E. faecalis FJSWX25M1 induced low levels of cytokines in vitro and showed no beneficial effects. Therefore, we provided insight into the clinical application of probiotics in IBD treatment.

Keywords: Intestinal barrier, Gut commensals, Immunoregulation, Dextran sulfate sodium (DSS) colitis

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Received: 09 July 2022
Revised: 17 October 2022
Accepted: 27 October 2022
Published: 08 February 2024
Issue date: May 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

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This work was supported by the Natural Science Foundation of Jiangsu Province (BK20200084); The National Natural Science Foundation of China (U1903205 and 31972971); and Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.

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