Journal Home > Volume 13 , Issue 2
Food Science and Human Wellness 2024, 13(2): 1029-1039 https://doi.org/10.26599/FSHW.2022.9250089
Open Access | Issue | Published: 25 September 2023

A core-satellite self-assembled SERS aptasensor containing a “biological-silent region” Raman tag for the accurate and ultrasensitive detection of histamine

Show Author's Information Chen Chena,1,  Yingfang Zhanga,1,  Ximo Wanga,,  Xuguang Qiaoa,  Geoffrey I.N. Waterhouseb( ),  Zhixiang Xua( )
Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
School of Chemical Sciences, The University of Auckland, Auckland 1142, New Zealand

1 Yingfang Zhang and Chen Chen contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

Keywords:
Aptamer, Surface-enhanced Raman spectroscopy, Raman biological-silent region, Histamine detection, Universal SERS-tag
Cite this article:
Chen C, Zhang Y, Wang X, et al. A core-satellite self-assembled SERS aptasensor containing a “biological-silent region” Raman tag for the accurate and ultrasensitive detection of histamine. Food Science and Human Wellness, 2024, 13(2): 1029-1039. https://doi.org/10.26599/FSHW.2022.9250089
Download citation
EndNote(RIS)
BibTeX

1378

Views

263

Downloads

Citations

7

Crossref

2

WoS

6

Scopus

0

CSCD

Abstract Full text Electronic supplementary material About this article

Herein, a novel interference-free surface-enhanced Raman spectroscopy (SERS) strategy based on magnetic nanoparticles (MNPs) and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine. A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe3O4@Au MNPs (as the recognize probe for histamine) and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile (4-MBN) (Ag@4-MBN@Ag-c-DNA) as the SERS signal probe for the indirect detection of histamine. Under an applied magnetic field in the absence of histamine, the assembly gave an intense Raman signal at “Raman biological-silent” region due to 4-MBN. In the presence of histamine, the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe3O4@Au MNPs, thus decreasing the SERS signal. Under optimal conditions, an ultra-low limit of detection of 0.65 × 10-3 ng/mL and a linear range 10-2–105 ng/mL on the SERS aptasensor were obtained. The histamine content in four food samples were analyzed using the SERS aptasensor, with the results consistent with those determined by high performance liquid chromatography. The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.


menu
Abstract
Full text
Outline
Electronic supplementary material
About this article

A core-satellite self-assembled SERS aptasensor containing a “biological-silent region” Raman tag for the accurate and ultrasensitive detection of histamine

Show Author's information Chen Chena,1, Yingfang Zhanga,1, Ximo Wanga,, Xuguang Qiaoa, Geoffrey I.N. Waterhouseb( ), Zhixiang Xua( )
Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
School of Chemical Sciences, The University of Auckland, Auckland 1142, New Zealand

1 Yingfang Zhang and Chen Chen contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

Research Highlights

● A SERS aptasensor operating in “biological-silent region” was developed for histamine detection.

● This SERS strategy was based on magnetic-induced and aptamer-driven assemblies.

● Ag@4-MBN@Ag NPs were used to significantly amplify 4-mercaptobenzonitrile SERS signal.

● The linear range of aptasensor was 10-2–105 ng/mL, with a LOD of 0.65 × 10-3 ng/mL.

● The developed aptasensor allowed histamine quantification in a variety of foods.

Abstract

Herein, a novel interference-free surface-enhanced Raman spectroscopy (SERS) strategy based on magnetic nanoparticles (MNPs) and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine. A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe3O4@Au MNPs (as the recognize probe for histamine) and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile (4-MBN) (Ag@4-MBN@Ag-c-DNA) as the SERS signal probe for the indirect detection of histamine. Under an applied magnetic field in the absence of histamine, the assembly gave an intense Raman signal at “Raman biological-silent” region due to 4-MBN. In the presence of histamine, the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe3O4@Au MNPs, thus decreasing the SERS signal. Under optimal conditions, an ultra-low limit of detection of 0.65 × 10-3 ng/mL and a linear range 10-2–105 ng/mL on the SERS aptasensor were obtained. The histamine content in four food samples were analyzed using the SERS aptasensor, with the results consistent with those determined by high performance liquid chromatography. The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.

Keywords: Aptamer, Surface-enhanced Raman spectroscopy, Raman biological-silent region, Histamine detection, Universal SERS-tag

References(34)

[1]

N. Notipichet, S. Khumngern, J. Choosang, et al., An enzymatic histamine biosensor based on a screen-printed carbon electrode modified with a chitosan-gold nanoparticles composite cryogel on Prussian blue-coated multi-walled carbon nanotubes, Food Chem. 364 (2021) 130396. https://doi.org/10.1016/j.foodchem.2021.130396.
DOI Google Scholar
[2]

S. Xu, F. Wu, F. Mu, et al., The preparation of Fe-based peroxidase mimetic nanozymes and for the electrochemical detection of histamine, J. Electroanal. Chem. 908 (2022) 116088. https://doi.org/10.1016/j.jelechem.2022.116088.
DOI Google Scholar
[3]

A. Parvenn, A. K. Kaushik, J. Prakash, A novel biosensing of histamine based on liquid crystal through dielectric and electro-optical approaches, Mater. Lett. 309 (2022) 131323. https://doi.org/10.1016/j.matlet.2021.131323.
DOI Google Scholar
[4]

V. Simat, P. Dalgaard, Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood, LWT-Food Sci. Technol. 44 (2011) 399-406. https://doi.org/10.1016/j.lwt.2010.08.011.
DOI Google Scholar
[5]

H. Harmoko, R.E. Kartasasmita, H. Munawar, et al., Determination of histamine in different compositions of commercially canned fish in Indonesia by modified QuEChERS and LC-MS/MS, J. Food Compos. Anal. 105 (2022) 104256. https://doi.org/10.1016/j.jfca.2021.104256.
DOI Google Scholar
[6]

L. Vitali, A.C. Valese, M.S. Azevedo, et al., Development of a fast and selective separation method to determine histamine in tuna fish samples using capillary zone electrophoresis, Talanta 106 (2013) 181-185. https://doi.org/10.1016/j.talanta.2012.12.020.
DOI Google Scholar
[7]

B. Zhang, W. Sheng, Y. Liu, et al., Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of tyramine and histamine in food samples, Anal. Chim. Acta. 1130 (2020) 117-125. https://doi.org/10.1016/j.aca.2020.07.043.
DOI Google Scholar
[8]

Y.F. Li, Z.Z. Lin, C.Y. Hong, et al., Histamine detection in fish samples based on indirect competitive ELISA method using iron-cobalt co-doped carbon dots labeled histamine antibody, Food Chem. 345 (2021) 128812. https://doi.org/10.1016/j.foodchem.2020.128812.
DOI Google Scholar
[9]

L. Zhang, J. Sun, Y. Fang, et al., Tribo-electrophoresis preconcentration enhanced ultra-sensitive SERS detection, Nano Energy 98 (2022) 107239. https://doi.org/10.1016/j.nanoen.2022.107239.
DOI Google Scholar
[10]

L. Zhang, J. Sun, Z. Li, et al., Coherent enhancement of dual-path-excited remote SERS, ACS Appl. Mater. Inter. 12 (2020) 32746-32751. https://doi.org/10.1021/acsami.0c07939.
DOI Google Scholar
[11]

L. Song, J. Li, H. Li, et al., Highly sensitive SERS detection for aflatoxin B1 and ochratoxin A based on aptamer-functionalized photonic crystal microsphere array, Sensor. Actuat. B-Chem. 364 (2022) 131778. https://doi.org/10.1016/j.snb.2022.131778.
DOI Google Scholar
[12]

Y. Yang, A.O`Riordan, P. Lovera, Highly sensitive pesticide detection using electrochemically prepared silver-gum Arabic nanocluster SERS substrates, Sensor. Actuat. B-Chem. 364 (2022) 131851. https://doi.org/10.1016/j.snb.2022.131851.
DOI Google Scholar
[13]

T. Xie, Z. Cao, Y. Li, et al., Highly sensitive SERS substrates with multi-hot spots for on-site detection of pesticide residues, Food Chem. 381 (2022) 132208. https://doi.org/10.1016/j.foodchem.2022.132208.
DOI Google Scholar
[14]

L.F. Tadesse, C.S. Ho, D.H. Chen, et al., Plasmonic and electrostatic interactions enable uniformly enhanced liquid bacterial surface-enhanced Raman scattering (SERS), Nano Lett. 20 (2020) 7655-7661. https://doi.org/10.1021/acs.nanolett.0c03189.
DOI Google Scholar
[15]

C. Zhang, S. Chen, Z. Jiang, et al., Highly sensitive and reproducible SERS substrates based on ordered micropyramid array and silver nanoparticles, ACS Appl. Mater. Inter. 13 (2021) 29222-29229. https://doi.org/10.1021/acsami.1c08712.
DOI Google Scholar
[16]

D. Zhang, H. Pu, L. Huang, et al., Advances in flexible surface-enhanced Raman scattering (SERS) substrates for nondestructive food detection: fundamentals and recent applications, Trends Food Sci. Tech. 109 (2021) 690-701. https://doi.org/10.1016/j.tifs.2021.01.058.
DOI Google Scholar
[17]

X.X. Han, L. Chen, W. Ji, et al., Label-free indirect immunoassay using an avidin-induced surface-enhanced Raman scattering substrate, Small 7 (2011) 316-320. https://doi.org/10.1002/smll.201001936.
DOI Google Scholar
[18]

M. Avella-Oliver, R. Puchades, S. Wachsmann-Hogiu, et al., Label-free SERS analysis of proteins and exosomes with large-scale substrates from recordable compact disks, Sensor. Actuat. B-Chem. 252 (2017) 657-662. https://doi.org/10.1016/j.snb.2017.06.058.
DOI Google Scholar
[19]

Z. Zhou, R. Xiao, S. Cheng, et al., A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognition, Anal. Chim. Acta. 1160 (2021) 338421. https://doi.org/10.1016/j.aca.2021.338421.
DOI Google Scholar
[20]

X.B. Huang, S.H. Wu, H.C. Hu, et al., Aunanostar@4-MBA@Au core-shell nanostructure coupled with exonuclease Ⅲ-assisted cycling amplification for ultrasensitive SERS detection of ochratoxin A, ACS Sensors 5 (2020) 2636-2643. https://doi.org/10.1021/acssensors.0c01162.
DOI Google Scholar
[21]

L. Huang, D.W. Sun, Z.Wu, et al., Reproducible, shelf-stable, and bioaffinity SERS nanotags inspired by multivariate polyphenolic chemistry for bacterial identification, Anal. Chim. Acta. 1167 (2021) 338570. https://doi.org/10.1016/j.aca.2021.338570.
DOI Google Scholar
[22]

X.R. Bai, L.H. Wang, J.Q. Ren, et al., Accurate clinical diagnosis of liver cancer based on simultaneous detection of ternary specific antigens by magnetic induced mixing SERS emissions, Anal. Chem. 91 (2019) 2955-2963. https://doi.org/10.1021/acs.analchem.8b05153.
DOI Google Scholar
[23]

S. Ma, Q. Li, Y. Yin, et al., Interference-free surface-enhanced Raman scattering tags for single-cell molecular imaging with a high signal-to background ratio, Small 13 (2017) 1603340. https://doi.org/10.1002/smll.201603340.
DOI Google Scholar
[24]

L. Xing, Y. Xiahou, P. Zhang, et al., Size control synthesis of monodisperse, quasi-spherical silver nanoparticles to realize surface-enhanced Raman scattering uniformity and reproducibility, ACS Appl. Mater. Inter. 11 (2019) 17637-17646. https://doi.org/10.1021/acsami.9b02052.
DOI Google Scholar
[25]

K. Wang, D.W. Sun, H. Pu, et al,, A rapid dual-channel readout approach for sensing carbendazim with 4-aminobenzenethiol-functionalized core-shell Au@Ag nanoparticles, The Analyst 145 (2020) 1801-1809. https://doi.org/10.1039/C9AN02185J.
DOI Google Scholar
[26]

Q. Chen, M. Yang, X. Yang, et al., A large Raman scattering cross-section molecular embedded SERS aptasensor for ultrasensitive Aflatoxin B1 detection using CS-Fe3O4 for signal enrichment, Spectrochim. Acta A 189 (2018) 147-153. https://doi.org/10.1016/j.saa.2017.08.029.
DOI Google Scholar
[27]

J. Wu, X. Zhou, P. Li, et al., Ultrasensitive and simultaneous SERS detection of multiplex microRNA using fractal gold nanotags for early diagnosis and prognosis of hepatocellular carcinoma, Anal. Chem. 93 (2021) 8799-8809. https://doi.org/10.1021/acs.analchem.1c00478.
DOI Google Scholar
[28]

N. Duan, C. Li, X. Zhu, et al., Simultaneous coupled with separate SELEX for heterocyclic biogenic amine-specific aptamers screening and their application in establishment of an effective aptasensor, Sensor. Actuat. B-Chem. 352 (2022) 130985. https://doi.org/10.1016/j.snb.2021.130985.
DOI Google Scholar
[29]

S. Cheng, Z. Tu, S. Zheng, et al., An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags, Anal. Chim. Acta 1187 (2021) 339155. https://doi.org/10.1016/j.aca.2021.339155.
DOI Google Scholar
[30]

Y. Pang, N. Wan, L. Shi, et al., Dual-recognition surface-enhanced Raman scattering (SERS) biosensor for pathogenic bacteria detection by using vancomycin-SERS tags and aptamer-Fe3O4@Au, Anal. Chim. Acta 1077 (2019) 288-296. https://doi.org/10.1016/j.aca.2019.05.059.
DOI Google Scholar
[31]

W. Wu, Z. Jing, X. Yu, et al., Recent advances in screening aquatic products for Vibrio spp., TrAC-Trend. Anal. Chem. 111 (2019) 239-251. https://doi.org/10.1016/j.trac.2018.11.043.
DOI Google Scholar
[32]

S. Xuan, F. Wang, Y. X. J. Wang, et al., Facile synthesis of size-controllable monodispersed ferrite nanospheres, J. Mater. Chem. 20 (2010) 5086-5094. https://doi.org/10.1039/C0JM00159G.
DOI Google Scholar
[33]

X. Liu, X. Yang, K. Li, et al., Fe3O4@Au SERS tags-based lateral flow assay for simultaneous detection of serum amyloid A and C-reactive protein in unprocessed blood sample, Sensor. Actuat. B-Chem. 320 (2020) 128350. https://doi.org/10.1016/j.snb.2020.128350.
DOI Google Scholar
[34]

C. Wang, J. Wang, M. Li, et al., A rapid SERS method for label-free bacteria detection using polyethylenimine-modified Au-coated magnetic microspheres and Au@Ag nanoparticles, Analyst. 141 (2016) 6226-6238. https://doi.org/10.1039/c6an01105e.
DOI Google Scholar
File
fshw-13-2-1029_ESM.docx (2.9 MB)
Publication history
Copyright
Acknowledgements
Rights and permissions

Publication history

Received: 29 August 2022
Revised: 08 October 2022
Accepted: 08 November 2022
Published: 25 September 2023
Issue date: March 2024

Copyright

© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

Acknowledgement

This work was financially supported by the National Natural Science Foundation of China (31972149). GINW acknowledges funding support from the MacDiarmid Institute for Advanced Materials and Nanotechnology and the Dodd-Walls Centre for Photonic and Quantum Technologies.

Rights and permissions

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Return